Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 127-137 |
| Number of pages | 11 |
| Journal | FEMS Microbiology Letters |
| Volume | 310 |
| Issue number | 2 |
| Early online date | 9 Jul 2010 |
| DOIs | |
| Publication status | Published - Sep 2010 |
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Keywords
- amino acid sequence
- animals
- cell line
- fish diseases
- gene expression profiling
- gene expression regulation
- host-parasite interactions
- infection
- molecular sequence data
- Oncorhynchus mykiss
- protein transport
- protozoan proteins
- saprolegnia
Cite this
The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells. / van West, Pieter; de Bruijn, Irene; Minor, Kirsty L; Phillips, Andrew J; Robertson, Emma J; Wawra, Stephan; Bain, Judith; Anderson, Victoria L; Secombes, Chris J.
In: FEMS Microbiology Letters, Vol. 310, No. 2, 09.2010, p. 127-137.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells
AU - van West, Pieter
AU - de Bruijn, Irene
AU - Minor, Kirsty L
AU - Phillips, Andrew J
AU - Robertson, Emma J
AU - Wawra, Stephan
AU - Bain, Judith
AU - Anderson, Victoria L
AU - Secombes, Chris J
PY - 2010/9
Y1 - 2010/9
N2 - The fish pathogenic oomycete Saprolegnia parasitica causes the disease Saprolegniosis in salmonids and other freshwater fish, resulting in considerable economic losses in aquaculture. Very little is known about the molecular and cellular mechanisms underlying the infection process of fish pathogenic oomycetes. In order to investigate the interaction in detail, an in vitro infection assay using an Oncorhynchus mykiss (rainbow trout) cell line (RTG-2) was developed. In a zoospore/cyst cDNA library, we identified the ORF SpHtp1, which encodes a secreted protein containing an RxLR motif. Detailed expression analysis indicated that SpHtp1 is highly expressed in zoospores/cysts from S. parasitica and in the very early stages of infection on RTG-2 cells, when compared with in vitro-grown mycelium. Moreover, the protein, SpHtp1, was found to translocate into the RTG-2 trout cells, during the interaction with S. parasitica, and also when the RTG-2 cells were treated with recombinant SpHtp1 fused to a C-terminal His-tag. These findings suggest that protein translocation could play an important role in Saprolegniosis.
AB - The fish pathogenic oomycete Saprolegnia parasitica causes the disease Saprolegniosis in salmonids and other freshwater fish, resulting in considerable economic losses in aquaculture. Very little is known about the molecular and cellular mechanisms underlying the infection process of fish pathogenic oomycetes. In order to investigate the interaction in detail, an in vitro infection assay using an Oncorhynchus mykiss (rainbow trout) cell line (RTG-2) was developed. In a zoospore/cyst cDNA library, we identified the ORF SpHtp1, which encodes a secreted protein containing an RxLR motif. Detailed expression analysis indicated that SpHtp1 is highly expressed in zoospores/cysts from S. parasitica and in the very early stages of infection on RTG-2 cells, when compared with in vitro-grown mycelium. Moreover, the protein, SpHtp1, was found to translocate into the RTG-2 trout cells, during the interaction with S. parasitica, and also when the RTG-2 cells were treated with recombinant SpHtp1 fused to a C-terminal His-tag. These findings suggest that protein translocation could play an important role in Saprolegniosis.
KW - amino acid sequence
KW - animals
KW - cell line
KW - fish diseases
KW - gene expression profiling
KW - gene expression regulation
KW - host-parasite interactions
KW - infection
KW - molecular sequence data
KW - Oncorhynchus mykiss
KW - protein transport
KW - protozoan proteins
KW - saprolegnia
U2 - 10.1111/j.1574-6968.2010.02055.x
DO - 10.1111/j.1574-6968.2010.02055.x
M3 - Article
C2 - 20659163
VL - 310
SP - 127
EP - 137
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -