The regulation of intracellular pH in bacteria

I R Booth, T A Krulwich, E Padan, J B Stock, G M Cook, V Skulachev, G N Bennett, W Epstein, J L Slonczewski, R J Rowbury, A Matin, J W Foster, R K Poole, W N Konings, G Schafer, P Dimroth

Research output: Chapter in Book/Report/Conference proceedingChapter

41 Citations (Scopus)

Abstract

The regulation of intracellular pH (pH(i)) in bacterial cells is achieved through control over cation (and anion) permeability. In addition to the active components of homeostasis there are contributions from essentially passive elements, such as the lipid composition of the membrane and the buffering capacity of the cytoplasm. Active homeostasis involves control over the movement of K+, Na+ and H+. Alterations in the membrane permeability for any of these cations may cause perturbation of homeostasis. In Escherichia coli this is exemplified by the controlled activation of K+ efflux systems by glutathione adducts leading to temporary acidification of the cytoplasm. This is achieved by sophisticated control over the KefB and KefC systems, and is tightly integrated with glutathione-dependent detoxification mechanisms. Such control over pH(i) facilitates survival of the cell following exposure to toxic electrophiles. The components of pH homeostasis will be reviewed and the molecular mechanisms, and role of, the KefB and KefC systems will be discussed.

Original languageEnglish
Title of host publicationNovartis Foundation Symposium 221
Subtitle of host publicationBacterial Responses to Ph
EditorsDerek J. Chawdick, Gail Cardew
Place of PublicationChichester, England
PublisherWiley-Blackwell
Pages19-37
Number of pages19
Volume221
ISBN (Electronic)9780470515631
ISBN (Print)9780471985990
Publication statusPublished - 1999

Publication series

NameNovartis Foundation Symposia
ISSN (Electronic)1935-4657

Keywords

  • ESCHERICHIA-COLI-CELLS
  • N-ETHYLMALEIMIDE
  • CYTOPLASMIC PH
  • SALMONELLA-TYPHIMURIUM
  • POTASSIUM-TRANSPORT
  • GROWTH TEMPERATURE
  • WEAK ACIDS
  • METHYLGLYOXAL
  • K+
  • SURVIVAL

Cite this

Booth, I. R., Krulwich, T. A., Padan, E., Stock, J. B., Cook, G. M., Skulachev, V., ... Dimroth, P. (1999). The regulation of intracellular pH in bacteria. In D. J. Chawdick, & G. Cardew (Eds.), Novartis Foundation Symposium 221: Bacterial Responses to Ph (Vol. 221, pp. 19-37). (Novartis Foundation Symposia). Chichester, England: Wiley-Blackwell .

The regulation of intracellular pH in bacteria. / Booth, I R ; Krulwich, T A ; Padan, E ; Stock, J B ; Cook, G M ; Skulachev, V ; Bennett, G N ; Epstein, W ; Slonczewski, J L ; Rowbury, R J ; Matin, A ; Foster, J W ; Poole, R K ; Konings, W N ; Schafer, G ; Dimroth, P .

Novartis Foundation Symposium 221: Bacterial Responses to Ph. ed. / Derek J. Chawdick; Gail Cardew. Vol. 221 Chichester, England : Wiley-Blackwell , 1999. p. 19-37 (Novartis Foundation Symposia).

Research output: Chapter in Book/Report/Conference proceedingChapter

Booth, IR, Krulwich, TA, Padan, E, Stock, JB, Cook, GM, Skulachev, V, Bennett, GN, Epstein, W, Slonczewski, JL, Rowbury, RJ, Matin, A, Foster, JW, Poole, RK, Konings, WN, Schafer, G & Dimroth, P 1999, The regulation of intracellular pH in bacteria. in DJ Chawdick & G Cardew (eds), Novartis Foundation Symposium 221: Bacterial Responses to Ph. vol. 221, Novartis Foundation Symposia, Wiley-Blackwell , Chichester, England, pp. 19-37.
Booth IR, Krulwich TA, Padan E, Stock JB, Cook GM, Skulachev V et al. The regulation of intracellular pH in bacteria. In Chawdick DJ, Cardew G, editors, Novartis Foundation Symposium 221: Bacterial Responses to Ph. Vol. 221. Chichester, England: Wiley-Blackwell . 1999. p. 19-37. (Novartis Foundation Symposia).
Booth, I R ; Krulwich, T A ; Padan, E ; Stock, J B ; Cook, G M ; Skulachev, V ; Bennett, G N ; Epstein, W ; Slonczewski, J L ; Rowbury, R J ; Matin, A ; Foster, J W ; Poole, R K ; Konings, W N ; Schafer, G ; Dimroth, P . / The regulation of intracellular pH in bacteria. Novartis Foundation Symposium 221: Bacterial Responses to Ph. editor / Derek J. Chawdick ; Gail Cardew. Vol. 221 Chichester, England : Wiley-Blackwell , 1999. pp. 19-37 (Novartis Foundation Symposia).
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AU - Bennett, G N

AU - Epstein, W

AU - Slonczewski, J L

AU - Rowbury, R J

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AB - The regulation of intracellular pH (pH(i)) in bacterial cells is achieved through control over cation (and anion) permeability. In addition to the active components of homeostasis there are contributions from essentially passive elements, such as the lipid composition of the membrane and the buffering capacity of the cytoplasm. Active homeostasis involves control over the movement of K+, Na+ and H+. Alterations in the membrane permeability for any of these cations may cause perturbation of homeostasis. In Escherichia coli this is exemplified by the controlled activation of K+ efflux systems by glutathione adducts leading to temporary acidification of the cytoplasm. This is achieved by sophisticated control over the KefB and KefC systems, and is tightly integrated with glutathione-dependent detoxification mechanisms. Such control over pH(i) facilitates survival of the cell following exposure to toxic electrophiles. The components of pH homeostasis will be reviewed and the molecular mechanisms, and role of, the KefB and KefC systems will be discussed.

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KW - GROWTH TEMPERATURE

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