The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men

S R Gray, M Clifford, R Lancaster, M Leggate, M Davies, M A Nimmo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The formation of an interleukin-6/IL-6 receptor (IL-6/IL-6R) complex increases the biological activity and half-life of IL-6, with its response to exercise currently unknown. The aim of the present study was to determine the response of circulating levels of the IL-6/IL-6R complex to exercise. Nine healthy male participants performed 1 h of cycling exercise at a workload required to elicit 90% lactate threshold. Venous blood samples were taken at rest, immediately post-exercise and after 1.5 h rest. Hemoglobin concentration and hematocrit were measured to estimate changes in plasma volume during exercise. The concentrations of IL-6, sIL-6R and the IL-6/IL-6R complex were measured via ELISA and corrected for changes in plasma volume. A single bout of acute exercise resulted in a 5-fold increase (P < 0.05) in IL-6 and a 1.2-fold increase (P < 0.05) in sIL-6R immediately after exercise. These returned to baseline at 1.5 h after the cessation of exercise. There was a 2.1-fold increase (P < 0.05) in the levels of the IL-6/IL-6R complex post-exercise with levels remaining 1.8 times elevated (P < 0.05) after 1.5 h rest. The present study has demonstrated, for the first time, that circulating levels of the IL-6/IL-6R complex are increased in response to acute exercise in young males.
Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalCytokine
Volume47
Issue number2
Early online date13 Jun 2009
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Interleukin-6 Receptors
Interleukin-6
Exercise
Plasma Volume
Plasmas
Bioactivity
Workload
Hematocrit
Lactic Acid
Hemoglobins
Blood
Half-Life
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay

Keywords

  • adult
  • exercise
  • humans
  • interleukin-6
  • male
  • receptors, interleukin-6
  • trans-signalling
  • cytokines
  • cycling

Cite this

Gray, S. R., Clifford, M., Lancaster, R., Leggate, M., Davies, M., & Nimmo, M. A. (2009). The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men. Cytokine, 47(2), 98-102. https://doi.org/10.1016/j.cyto.2009.05.011

The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men. / Gray, S R; Clifford, M; Lancaster, R; Leggate, M; Davies, M; Nimmo, M A.

In: Cytokine, Vol. 47, No. 2, 08.2009, p. 98-102.

Research output: Contribution to journalArticle

Gray, SR, Clifford, M, Lancaster, R, Leggate, M, Davies, M & Nimmo, MA 2009, 'The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men', Cytokine, vol. 47, no. 2, pp. 98-102. https://doi.org/10.1016/j.cyto.2009.05.011
Gray, S R ; Clifford, M ; Lancaster, R ; Leggate, M ; Davies, M ; Nimmo, M A. / The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men. In: Cytokine. 2009 ; Vol. 47, No. 2. pp. 98-102.
@article{d030cd3119324bce95b5913181e6cb71,
title = "The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men",
abstract = "The formation of an interleukin-6/IL-6 receptor (IL-6/IL-6R) complex increases the biological activity and half-life of IL-6, with its response to exercise currently unknown. The aim of the present study was to determine the response of circulating levels of the IL-6/IL-6R complex to exercise. Nine healthy male participants performed 1 h of cycling exercise at a workload required to elicit 90{\%} lactate threshold. Venous blood samples were taken at rest, immediately post-exercise and after 1.5 h rest. Hemoglobin concentration and hematocrit were measured to estimate changes in plasma volume during exercise. The concentrations of IL-6, sIL-6R and the IL-6/IL-6R complex were measured via ELISA and corrected for changes in plasma volume. A single bout of acute exercise resulted in a 5-fold increase (P < 0.05) in IL-6 and a 1.2-fold increase (P < 0.05) in sIL-6R immediately after exercise. These returned to baseline at 1.5 h after the cessation of exercise. There was a 2.1-fold increase (P < 0.05) in the levels of the IL-6/IL-6R complex post-exercise with levels remaining 1.8 times elevated (P < 0.05) after 1.5 h rest. The present study has demonstrated, for the first time, that circulating levels of the IL-6/IL-6R complex are increased in response to acute exercise in young males.",
keywords = "adult, exercise, humans, interleukin-6, male, receptors, interleukin-6, trans-signalling, cytokines, cycling",
author = "Gray, {S R} and M Clifford and R Lancaster and M Leggate and M Davies and Nimmo, {M A}",
year = "2009",
month = "8",
doi = "10.1016/j.cyto.2009.05.011",
language = "English",
volume = "47",
pages = "98--102",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The response of circulating levels of the interleukin-6/interleukin-6 receptor complex to exercise in young men

AU - Gray, S R

AU - Clifford, M

AU - Lancaster, R

AU - Leggate, M

AU - Davies, M

AU - Nimmo, M A

PY - 2009/8

Y1 - 2009/8

N2 - The formation of an interleukin-6/IL-6 receptor (IL-6/IL-6R) complex increases the biological activity and half-life of IL-6, with its response to exercise currently unknown. The aim of the present study was to determine the response of circulating levels of the IL-6/IL-6R complex to exercise. Nine healthy male participants performed 1 h of cycling exercise at a workload required to elicit 90% lactate threshold. Venous blood samples were taken at rest, immediately post-exercise and after 1.5 h rest. Hemoglobin concentration and hematocrit were measured to estimate changes in plasma volume during exercise. The concentrations of IL-6, sIL-6R and the IL-6/IL-6R complex were measured via ELISA and corrected for changes in plasma volume. A single bout of acute exercise resulted in a 5-fold increase (P < 0.05) in IL-6 and a 1.2-fold increase (P < 0.05) in sIL-6R immediately after exercise. These returned to baseline at 1.5 h after the cessation of exercise. There was a 2.1-fold increase (P < 0.05) in the levels of the IL-6/IL-6R complex post-exercise with levels remaining 1.8 times elevated (P < 0.05) after 1.5 h rest. The present study has demonstrated, for the first time, that circulating levels of the IL-6/IL-6R complex are increased in response to acute exercise in young males.

AB - The formation of an interleukin-6/IL-6 receptor (IL-6/IL-6R) complex increases the biological activity and half-life of IL-6, with its response to exercise currently unknown. The aim of the present study was to determine the response of circulating levels of the IL-6/IL-6R complex to exercise. Nine healthy male participants performed 1 h of cycling exercise at a workload required to elicit 90% lactate threshold. Venous blood samples were taken at rest, immediately post-exercise and after 1.5 h rest. Hemoglobin concentration and hematocrit were measured to estimate changes in plasma volume during exercise. The concentrations of IL-6, sIL-6R and the IL-6/IL-6R complex were measured via ELISA and corrected for changes in plasma volume. A single bout of acute exercise resulted in a 5-fold increase (P < 0.05) in IL-6 and a 1.2-fold increase (P < 0.05) in sIL-6R immediately after exercise. These returned to baseline at 1.5 h after the cessation of exercise. There was a 2.1-fold increase (P < 0.05) in the levels of the IL-6/IL-6R complex post-exercise with levels remaining 1.8 times elevated (P < 0.05) after 1.5 h rest. The present study has demonstrated, for the first time, that circulating levels of the IL-6/IL-6R complex are increased in response to acute exercise in young males.

KW - adult

KW - exercise

KW - humans

KW - interleukin-6

KW - male

KW - receptors, interleukin-6

KW - trans-signalling

KW - cytokines

KW - cycling

U2 - 10.1016/j.cyto.2009.05.011

DO - 10.1016/j.cyto.2009.05.011

M3 - Article

VL - 47

SP - 98

EP - 102

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 2

ER -