The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

Susan J. Duthie, Yiannis Mavrommatis, Garry Rucklidge, Martin Reid, Gary Duncan, Mary P. Moyer, Lynn P. Pirie, Charles S. Bestwick

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

Original languageEnglish
Pages (from-to)3254-3266
Number of pages13
JournalJournal of Proteome Research
Volume7
Issue number8
Early online date3 Jul 2008
DOIs
Publication statusPublished - Aug 2008

Keywords

  • folic acid deficiency
  • human colonocytes
  • NCM460
  • genomic stability
  • DNA repair
  • apoptosis
  • proteomics
  • gene-expression profiles
  • epithelial-cell line
  • DNA excision-repair
  • breast-cancer
  • uracil misincorporation
  • colon carcinogenesis
  • colorectal-cancer
  • mass-spectrometry
  • carcinoma-cells
  • protein

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