The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

Susan J. Duthie, Yiannis Mavrommatis, Garry Rucklidge, Martin Reid, Gary Duncan, Mary P. Moyer, Lynn P. Pirie, Charles S. Bestwick

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

Original languageEnglish
Pages (from-to)3254-3266
Number of pages13
JournalJournal of Proteome Research
Volume7
Issue number8
Early online date3 Jul 2008
DOIs
Publication statusPublished - Aug 2008

Keywords

  • folic acid deficiency
  • human colonocytes
  • NCM460
  • genomic stability
  • DNA repair
  • apoptosis
  • proteomics
  • gene-expression profiles
  • epithelial-cell line
  • DNA excision-repair
  • breast-cancer
  • uracil misincorporation
  • colon carcinogenesis
  • colorectal-cancer
  • mass-spectrometry
  • carcinoma-cells
  • protein

Cite this

The response of human colonocytes to folate deficiency in vitro : functional and proteomic analyses. / Duthie, Susan J.; Mavrommatis, Yiannis; Rucklidge, Garry; Reid, Martin; Duncan, Gary; Moyer, Mary P.; Pirie, Lynn P.; Bestwick, Charles S.

In: Journal of Proteome Research, Vol. 7, No. 8, 08.2008, p. 3254-3266.

Research output: Contribution to journalArticle

Duthie, SJ, Mavrommatis, Y, Rucklidge, G, Reid, M, Duncan, G, Moyer, MP, Pirie, LP & Bestwick, CS 2008, 'The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses', Journal of Proteome Research, vol. 7, no. 8, pp. 3254-3266. https://doi.org/10.1021/pr700751y
Duthie, Susan J. ; Mavrommatis, Yiannis ; Rucklidge, Garry ; Reid, Martin ; Duncan, Gary ; Moyer, Mary P. ; Pirie, Lynn P. ; Bestwick, Charles S. / The response of human colonocytes to folate deficiency in vitro : functional and proteomic analyses. In: Journal of Proteome Research. 2008 ; Vol. 7, No. 8. pp. 3254-3266.
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