The response of human colonocytes to folate deficiency in vitro

functional and proteomic analyses

Susan J. Duthie, Yiannis Mavrommatis, Garry Rucklidge, Martin Reid, Gary Duncan, Mary P. Moyer, Lynn P. Pirie, Charles S. Bestwick

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].

Original languageEnglish
Pages (from-to)3254-3266
Number of pages13
JournalJournal of Proteome Research
Volume7
Issue number8
Early online date3 Jul 2008
DOIs
Publication statusPublished - Aug 2008

Keywords

  • folic acid deficiency
  • human colonocytes
  • NCM460
  • genomic stability
  • DNA repair
  • apoptosis
  • proteomics
  • gene-expression profiles
  • epithelial-cell line
  • DNA excision-repair
  • breast-cancer
  • uracil misincorporation
  • colon carcinogenesis
  • colorectal-cancer
  • mass-spectrometry
  • carcinoma-cells
  • protein

Cite this

Duthie, S. J., Mavrommatis, Y., Rucklidge, G., Reid, M., Duncan, G., Moyer, M. P., ... Bestwick, C. S. (2008). The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses. Journal of Proteome Research, 7(8), 3254-3266. https://doi.org/10.1021/pr700751y

The response of human colonocytes to folate deficiency in vitro : functional and proteomic analyses. / Duthie, Susan J.; Mavrommatis, Yiannis; Rucklidge, Garry; Reid, Martin; Duncan, Gary; Moyer, Mary P.; Pirie, Lynn P.; Bestwick, Charles S.

In: Journal of Proteome Research, Vol. 7, No. 8, 08.2008, p. 3254-3266.

Research output: Contribution to journalArticle

Duthie, SJ, Mavrommatis, Y, Rucklidge, G, Reid, M, Duncan, G, Moyer, MP, Pirie, LP & Bestwick, CS 2008, 'The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses', Journal of Proteome Research, vol. 7, no. 8, pp. 3254-3266. https://doi.org/10.1021/pr700751y
Duthie, Susan J. ; Mavrommatis, Yiannis ; Rucklidge, Garry ; Reid, Martin ; Duncan, Gary ; Moyer, Mary P. ; Pirie, Lynn P. ; Bestwick, Charles S. / The response of human colonocytes to folate deficiency in vitro : functional and proteomic analyses. In: Journal of Proteome Research. 2008 ; Vol. 7, No. 8. pp. 3254-3266.
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