The role of lipids in mechanosensation

Christos Pilotas, A Caroline E Dahl, Tim Rasmussen, Kozhinjampara R Mahendran, Terry K Smith, Phedra Marius, Joseph Gault, Thandiwe Banda, Akiko Rasmussen, Samantha Miller, Carol V Robinson, Hagan Bayley, Mark S P Sansom, Ian Rylance Booth, James H Naismith

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Abstract

The ability of proteins to sense membrane tension is pervasive in biology. A higher-resolution structure of the Escherichia coli small-conductance mechanosensitive channel MscS identifies alkyl chains inside pockets formed by the transmembrane helices (TMs). Purified MscS contains E. coli lipids, and fluorescence quenching demonstrates that phospholipid acyl chains exchange between bilayer and TM pockets. Molecular dynamics and biophysical analyses show that the volume of the pockets and thus the number of lipid acyl chains within them decreases upon channel opening. Phospholipids with one acyl chain per head group (lysolipids) displace normal phospholipids (with two acyl chains) from MscS pockets and trigger channel opening. We propose that the extent of acyl-chain interdigitation in these pockets determines the conformation of MscS. When interdigitation is perturbed by increased membrane tension or by lysolipids, the closed state becomes unstable, and the channel gates.
Original languageEnglish
Pages (from-to)991-998
Number of pages8
JournalNature Structural & Molecular Biology
Volume22
Issue number12
Early online date9 Nov 2015
DOIs
Publication statusPublished - Dec 2015

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    Pilotas, C., Dahl, A. C. E., Rasmussen, T., Mahendran, K. R., Smith, T. K., Marius, P., Gault, J., Banda, T., Rasmussen, A., Miller, S., Robinson, C. V., Bayley, H., Sansom, M. S. P., Booth, I. R., & Naismith, J. H. (2015). The role of lipids in mechanosensation. Nature Structural & Molecular Biology, 22(12), 991-998. https://doi.org/10.1038/nsmb.3120