The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli

Akiko Rasmussen, Tim Rasmussen, Michelle Diane Edwards, Daniela Sonja Schauer, Ulrike Schumann, Samantha Miller, Ian Rylance Booth

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tryptophan (Trp) residues play important roles in many proteins. In particular they are enriched in protein surfaces involved in protein docking and are often found in membrane proteins close to the lipid head groups. However, they are usually absent from the membrane domains of mechanosensitive channels. Three Trp residues occur naturally in the Escherichia coli MscS (MscS-Ec) protein: W16 lies in the periplasm, immediately before the first transmembrane span (TM1), whereas W240 and W251 lie at the subunit interfaces that create the cytoplasmic vestibule portals. The role of these residues in MscS function and stability were investigated using site-directed mutagenesis. Functional channels with altered properties were created when any of the Trp residues were replaced by another amino acid, with the greatest retention of function associated with phenylalanine (Phe) substitutions. Analysis of the fluorescence properties of purified mutant MscS proteins containing single Trp residues revealed that W16 and W251 are relatively inaccessible, whereas W240 is accessible to quenching agents. The data point to a significant role for W16 in the gating of MscS, and an essential role for W240 in MscS oligomer stability.

Original languageEnglish
Pages (from-to)10899-10908
Number of pages10
JournalBiochemistry
Volume46
Issue number38
Early online date24 Aug 2007
DOIs
Publication statusPublished - 25 Sep 2007

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Tryptophan
Escherichia coli
Membrane Proteins
Proteins
Periplasm
Mutagenesis
Mutant Proteins
Site-Directed Mutagenesis
Phenylalanine
Oligomers
Quenching
Substitution reactions
Fluorescence
Membranes
Lipids
Amino Acids

Keywords

  • cation-pi interactions
  • hot-spots
  • protein
  • fluorescence
  • interface
  • bacteria
  • binding
  • stretch
  • energy
  • cells

Cite this

Rasmussen, A., Rasmussen, T., Edwards, M. D., Schauer, D. S., Schumann, U., Miller, S., & Booth, I. R. (2007). The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli. Biochemistry, 46(38), 10899-10908. https://doi.org/10.1021/BI701056K

The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli. / Rasmussen, Akiko; Rasmussen, Tim; Edwards, Michelle Diane; Schauer, Daniela Sonja; Schumann, Ulrike; Miller, Samantha; Booth, Ian Rylance.

In: Biochemistry, Vol. 46, No. 38, 25.09.2007, p. 10899-10908.

Research output: Contribution to journalArticle

Rasmussen, A, Rasmussen, T, Edwards, MD, Schauer, DS, Schumann, U, Miller, S & Booth, IR 2007, 'The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli' Biochemistry, vol. 46, no. 38, pp. 10899-10908. https://doi.org/10.1021/BI701056K
Rasmussen, Akiko ; Rasmussen, Tim ; Edwards, Michelle Diane ; Schauer, Daniela Sonja ; Schumann, Ulrike ; Miller, Samantha ; Booth, Ian Rylance. / The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli. In: Biochemistry. 2007 ; Vol. 46, No. 38. pp. 10899-10908.
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