The roles of Pax6 in the cornea, retina and olfactory epithelium of the developing mouse embryo

Jon Martin Collinson, J. Quinn, R. E. Hill, J. D. West

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The roles of Pax6 were investigated in the murine eye and the olfactory epithelium by analysing gene expression and distribution of Pax6(-/-) cells in Pax6(+/+) <-> Pax6(-/-) chimeras. It was found that between embryonic days E10.5 and E16.5 Pax6 is autonomously required for cells to contribute fully not only to the corneal epithelium, where Pax6 is expressed at high levels, but also to the to the corneal stroma and endothelium, where the protein is detected at very low levels. Pax6(-/-) cells contributed only poorly to the neural retina, forming small clumps of cells that were normally restricted to the ganglion cell layer at E16.5. Pax6(-/-) cells in the retinal pigment epithelium could express Trp2, a component of the pigmentation pathway, at E14.5 and a small number went on to differentiate and produce pigment at E16.5. The segregation and near-exclusion of mutant cells from the nasal epithelium mirrored the behaviour of mutant cells in other developmental contexts, particularly the lens, suggesting that common primary defects may be responsible for diverse Pax6-related phenotypes. (C) 2003 Elsevier Science (USA). All rights reserved.

Original languageEnglish
Pages (from-to)303-312
Number of pages9
JournalDevelopmental Biology
Volume255
Issue number2
Early online date8 Feb 2003
DOIs
Publication statusPublished - 15 Mar 2003

Keywords

  • chimera
  • chimaera
  • Pax6
  • eye development
  • olfactory epithelium
  • cornea
  • retina
  • lens
  • midbrain crest cells
  • segment abnormalities
  • lens differentiation
  • insitu hybridization
  • nasal development
  • optic vesicle
  • DNA-sequences
  • small-eye
  • gene
  • expression

Cite this

The roles of Pax6 in the cornea, retina and olfactory epithelium of the developing mouse embryo. / Collinson, Jon Martin; Quinn, J.; Hill, R. E.; West, J. D.

In: Developmental Biology, Vol. 255, No. 2, 15.03.2003, p. 303-312.

Research output: Contribution to journalArticle

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