The Staphylococcus aureus lipoprotein SitC colocalizes with Toll-like receptor 2 (TLR2) in murine keratinocytes and elicits intracellular TLR2 accumulation

P Müller, M Müller-Anstett, J Wagener, Q Gao, S Kaesler, M Schaller, T Biedermann, F Götz

Research output: Contribution to journalArticle

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Abstract

SitC is one of the predominant lipoproteins in Staphylococcus aureus. Recently, SitC was shown to be capable of stimulating Toll-like receptor 2 (TLR2), but the mechanism of TLR2 activation by SitC has not been analyzed in detail so far. In this study, we purified C-terminally His-tagged SitC (SitC-His) from Staphylococcus aureus. SitC-His induced interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-a) release in human monocytes and also NF-¿B activation in TLR2-transfected HEK293 cells, indicating TLR2-specific activation. SitC not only induced a TLR2-dependent release of IL-6 in primary murine keratinocytes (MKs) but also induced intracellular accumulation of TLR2, which was time and concentration dependent. Cy2-labeled SitC-His colocalized specifically with TLR2 in MKs and was also internalized in TLR2 knockout MKs, suggesting a TLR2-independent uptake. Neither activation nor colocalization of SitC-His was observed with TLR4 or Nod2. The results show that the native lipoprotein SitC-His specifically colocalizes with TLR2, is internalized by host cells, induces proinflammatory cytokines, and triggers intracellular accumulation of TLR2.
Original languageEnglish
Pages (from-to)4243-4250
Number of pages8
JournalInfection and Immunity
Volume78
Issue number10
DOIs
Publication statusPublished - Oct 2010

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Toll-Like Receptor 2
Keratinocytes
Lipoproteins
Staphylococcus aureus
Interleukin-6
HEK293 Cells
Monocytes
Tumor Necrosis Factor-alpha

Keywords

  • animals
  • bacterial proteins
  • cell line
  • cytokines
  • gene expression regulation, bacterial
  • humans
  • keratinocytes
  • lipoproteins
  • mice
  • mice, inbred C57BL
  • mice, knockout
  • Nod2 signaling adaptor protein
  • protein transport
  • specific pathogen-free organisms
  • Staphylococcus aureus
  • toll-like receptor 2

Cite this

The Staphylococcus aureus lipoprotein SitC colocalizes with Toll-like receptor 2 (TLR2) in murine keratinocytes and elicits intracellular TLR2 accumulation. / Müller, P; Müller-Anstett, M; Wagener, J; Gao, Q; Kaesler, S; Schaller, M; Biedermann, T; Götz, F.

In: Infection and Immunity, Vol. 78, No. 10, 10.2010, p. 4243-4250.

Research output: Contribution to journalArticle

Müller, P ; Müller-Anstett, M ; Wagener, J ; Gao, Q ; Kaesler, S ; Schaller, M ; Biedermann, T ; Götz, F. / The Staphylococcus aureus lipoprotein SitC colocalizes with Toll-like receptor 2 (TLR2) in murine keratinocytes and elicits intracellular TLR2 accumulation. In: Infection and Immunity. 2010 ; Vol. 78, No. 10. pp. 4243-4250.
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abstract = "SitC is one of the predominant lipoproteins in Staphylococcus aureus. Recently, SitC was shown to be capable of stimulating Toll-like receptor 2 (TLR2), but the mechanism of TLR2 activation by SitC has not been analyzed in detail so far. In this study, we purified C-terminally His-tagged SitC (SitC-His) from Staphylococcus aureus. SitC-His induced interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-a) release in human monocytes and also NF-¿B activation in TLR2-transfected HEK293 cells, indicating TLR2-specific activation. SitC not only induced a TLR2-dependent release of IL-6 in primary murine keratinocytes (MKs) but also induced intracellular accumulation of TLR2, which was time and concentration dependent. Cy2-labeled SitC-His colocalized specifically with TLR2 in MKs and was also internalized in TLR2 knockout MKs, suggesting a TLR2-independent uptake. Neither activation nor colocalization of SitC-His was observed with TLR4 or Nod2. The results show that the native lipoprotein SitC-His specifically colocalizes with TLR2, is internalized by host cells, induces proinflammatory cytokines, and triggers intracellular accumulation of TLR2.",
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AU - Gao, Q

AU - Kaesler, S

AU - Schaller, M

AU - Biedermann, T

AU - Götz, F

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KW - bacterial proteins

KW - cell line

KW - cytokines

KW - gene expression regulation, bacterial

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KW - keratinocytes

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KW - mice, inbred C57BL

KW - mice, knockout

KW - Nod2 signaling adaptor protein

KW - protein transport

KW - specific pathogen-free organisms

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