The symbiosis of Bacillus subtilis L-forms with Chinese cabbage seedlings inhibit conidial germination of Botrytis cinerea

R. Walker, C. M. J. Ferguson, Nuala Ann Booth, Eunice Jessie Allan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: To establish whether germination of Botrytis cinerea was affected by the symbiosis of Bacillus subtilis L-form bacteria with Chinese cabbage.

Methods and Results: Germinating seeds of Chinese cabbage were co-cultivated with either L-forms of Bacillus subtilis or 59,6 (w/v) mannitol by soaking for 3 h. Seeds were then washed in sterile water, sown on a minimal medium and incubated in controlled conditions. L-form symbiosis was detected over a time course by ELISA. Conidial germination of Botrytis cinerea was significantly reduced on cotyledonous leaves of L-form-treated plants compared with controls.

Conclusions: Symbiosis of B. subtilis L-form bacteria during seed germination of Chinese cabbage inhibits conidial germination in plants on subsequent exposure to Botrylis cinerea.

Significance and Impact of the Study: This is the first account of plant symbiosis with L-form, bacteria showing antagonism to a fungal plant pathogen. This has promising implications for the use of this L-form as a biocontrol agent.

Original languageEnglish
Pages (from-to)42-45
Number of pages3
JournalLetters in Applied Microbiology
Volume34
DOIs
Publication statusPublished - 2002

Keywords

  • PLANTS
  • DISEASE

Cite this

The symbiosis of Bacillus subtilis L-forms with Chinese cabbage seedlings inhibit conidial germination of Botrytis cinerea. / Walker, R.; Ferguson, C. M. J.; Booth, Nuala Ann; Allan, Eunice Jessie.

In: Letters in Applied Microbiology, Vol. 34, 2002, p. 42-45.

Research output: Contribution to journalArticle

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title = "The symbiosis of Bacillus subtilis L-forms with Chinese cabbage seedlings inhibit conidial germination of Botrytis cinerea",
abstract = "Aims: To establish whether germination of Botrytis cinerea was affected by the symbiosis of Bacillus subtilis L-form bacteria with Chinese cabbage.Methods and Results: Germinating seeds of Chinese cabbage were co-cultivated with either L-forms of Bacillus subtilis or 59,6 (w/v) mannitol by soaking for 3 h. Seeds were then washed in sterile water, sown on a minimal medium and incubated in controlled conditions. L-form symbiosis was detected over a time course by ELISA. Conidial germination of Botrytis cinerea was significantly reduced on cotyledonous leaves of L-form-treated plants compared with controls.Conclusions: Symbiosis of B. subtilis L-form bacteria during seed germination of Chinese cabbage inhibits conidial germination in plants on subsequent exposure to Botrylis cinerea.Significance and Impact of the Study: This is the first account of plant symbiosis with L-form, bacteria showing antagonism to a fungal plant pathogen. This has promising implications for the use of this L-form as a biocontrol agent.",
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author = "R. Walker and Ferguson, {C. M. J.} and Booth, {Nuala Ann} and Allan, {Eunice Jessie}",
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T1 - The symbiosis of Bacillus subtilis L-forms with Chinese cabbage seedlings inhibit conidial germination of Botrytis cinerea

AU - Walker, R.

AU - Ferguson, C. M. J.

AU - Booth, Nuala Ann

AU - Allan, Eunice Jessie

PY - 2002

Y1 - 2002

N2 - Aims: To establish whether germination of Botrytis cinerea was affected by the symbiosis of Bacillus subtilis L-form bacteria with Chinese cabbage.Methods and Results: Germinating seeds of Chinese cabbage were co-cultivated with either L-forms of Bacillus subtilis or 59,6 (w/v) mannitol by soaking for 3 h. Seeds were then washed in sterile water, sown on a minimal medium and incubated in controlled conditions. L-form symbiosis was detected over a time course by ELISA. Conidial germination of Botrytis cinerea was significantly reduced on cotyledonous leaves of L-form-treated plants compared with controls.Conclusions: Symbiosis of B. subtilis L-form bacteria during seed germination of Chinese cabbage inhibits conidial germination in plants on subsequent exposure to Botrylis cinerea.Significance and Impact of the Study: This is the first account of plant symbiosis with L-form, bacteria showing antagonism to a fungal plant pathogen. This has promising implications for the use of this L-form as a biocontrol agent.

AB - Aims: To establish whether germination of Botrytis cinerea was affected by the symbiosis of Bacillus subtilis L-form bacteria with Chinese cabbage.Methods and Results: Germinating seeds of Chinese cabbage were co-cultivated with either L-forms of Bacillus subtilis or 59,6 (w/v) mannitol by soaking for 3 h. Seeds were then washed in sterile water, sown on a minimal medium and incubated in controlled conditions. L-form symbiosis was detected over a time course by ELISA. Conidial germination of Botrytis cinerea was significantly reduced on cotyledonous leaves of L-form-treated plants compared with controls.Conclusions: Symbiosis of B. subtilis L-form bacteria during seed germination of Chinese cabbage inhibits conidial germination in plants on subsequent exposure to Botrylis cinerea.Significance and Impact of the Study: This is the first account of plant symbiosis with L-form, bacteria showing antagonism to a fungal plant pathogen. This has promising implications for the use of this L-form as a biocontrol agent.

KW - PLANTS

KW - DISEASE

U2 - 10.1046/j.1472-765x.2002.01037.x

DO - 10.1046/j.1472-765x.2002.01037.x

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EP - 45

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

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