The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt

Bruno Silvester Lopes; Mohamed H Al-Agamy; Muhammad A Ismail; Atef M Shibl; Ahmed A Al-Qahtani; Mohammed N Al-Ahdal; Ken J Forbes

doi:10.1016/j.ijmm.2015.07.007

The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt

Bruno Silvester Lopes, Mohamed H Al-Agamy, Muhammad A Ismail, Atef M Shibl, Ahmed A Al-Qahtani, Mohammed N Al-Ahdal, Ken J Forbes

Applied Medicine

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Carbapenem-resistant Acinetobacter spp. have been increasingly reported worldwide including Saudi Arabia and Egypt. We examined 64, non-repetitive, Acinetobacter baumannii isolates collected in 2013 and 2014 from four different medical centres (two from Saudi Arabia and two from Egypt). All the isolates were resistant to ceftazidime and ciprofloxacin. The intI1 harbouring blaGES-11 and aac-6'-1b was detected in 19% (n=12) of the isolates. ISAba1 over-expression of blaADC gene was observed in 65% (n=42) of isolates. Of all the isolates 19% (n=12) had ISAba1 upstream of the blaOXA-51-like gene, 69% (n=44) carried the blaOXA-23 gene within the Tn2006 structure, 8% (n=5) had blaOXA-24-like gene and 9% (n=6) harboured either blaVIM-2 or blaNDM-1 gene. Eighty nine percent (n=57) of isolates were resistant to imipenem and had an MIC of ≥8mg/L. Pulsed-field gel electrophoresis (PFGE) typing revealed the presence of 23 different PFGE. Three PFGE types were very widespread, ST236 (CC104) (PFGE type 1, n=15), ST208 (CC92) (PFGE type 2, n=10), ST884 (CC unassigned) (PFGE type 3, n=7) in and across all four medical centres. The blaOXA-23 gene was found to be present on a 60kb transferable plasmid in both PFGE type 1 and 2 but was absent in PFGE type 3. This is the first study to report on the emergence of ST236 in Saudi Arabia and Egypt, and spread of distinct carbapenem resistant A. baumannii clones belonging to ST884, ST945 and ST1096 in Saudi Arabia.

Original language	English
Pages (from-to)	581-588
Number of pages	8
Journal	International Journal of Medical Microbiology: IJMM
Volume	305
Issue number	6
Early online date	30 Jul 2015
DOIs	https://doi.org/10.1016/j.ijmm.2015.07.007
Publication status	Published - Sept 2015

Bibliographical note

Copyright © 2015 Elsevier GmbH. All rights reserved.

Acknowledgements
We are grateful for the BMedSc project grant to BSL and MAI by the University of Aberdeen that funded this work and to Murray Robb for helpful comments. MHAA and AMS would also like to extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-VPP-038.

Keywords

Acinetobacter baumannii
carbapenems
Middle-East
OXA-23
plasmid

Access to Document

10.1016/j.ijmm.2015.07.007Licence: Unspecified

Cite this

@article{b44e2bd7199944da97ea42adfce90694,

title = "The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt",

abstract = "Carbapenem-resistant Acinetobacter spp. have been increasingly reported worldwide including Saudi Arabia and Egypt. We examined 64, non-repetitive, Acinetobacter baumannii isolates collected in 2013 and 2014 from four different medical centres (two from Saudi Arabia and two from Egypt). All the isolates were resistant to ceftazidime and ciprofloxacin. The intI1 harbouring blaGES-11 and aac-6'-1b was detected in 19% (n=12) of the isolates. ISAba1 over-expression of blaADC gene was observed in 65% (n=42) of isolates. Of all the isolates 19% (n=12) had ISAba1 upstream of the blaOXA-51-like gene, 69% (n=44) carried the blaOXA-23 gene within the Tn2006 structure, 8% (n=5) had blaOXA-24-like gene and 9% (n=6) harboured either blaVIM-2 or blaNDM-1 gene. Eighty nine percent (n=57) of isolates were resistant to imipenem and had an MIC of ≥8mg/L. Pulsed-field gel electrophoresis (PFGE) typing revealed the presence of 23 different PFGE. Three PFGE types were very widespread, ST236 (CC104) (PFGE type 1, n=15), ST208 (CC92) (PFGE type 2, n=10), ST884 (CC unassigned) (PFGE type 3, n=7) in and across all four medical centres. The blaOXA-23 gene was found to be present on a 60kb transferable plasmid in both PFGE type 1 and 2 but was absent in PFGE type 3. This is the first study to report on the emergence of ST236 in Saudi Arabia and Egypt, and spread of distinct carbapenem resistant A. baumannii clones belonging to ST884, ST945 and ST1096 in Saudi Arabia.",

keywords = "Acinetobacter baumannii, carbapenems, Middle-East, OXA-23, plasmid",

author = "Lopes, {Bruno Silvester} and Al-Agamy, {Mohamed H} and Ismail, {Muhammad A} and Shibl, {Atef M} and Al-Qahtani, {Ahmed A} and Al-Ahdal, {Mohammed N} and Forbes, {Ken J}",

note = "Copyright {\textcopyright} 2015 Elsevier GmbH. All rights reserved. Acknowledgements We are grateful for the BMedSc project grant to BSL and MAI by the University of Aberdeen that funded this work and to Murray Robb for helpful comments. MHAA and AMS would also like to extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-VPP-038.",

year = "2015",

month = sep,

doi = "10.1016/j.ijmm.2015.07.007",

language = "English",

volume = "305",

pages = "581--588",

journal = "International Journal of Medical Microbiology: IJMM",

issn = "1438-4221",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "6",

}

TY - JOUR

T1 - The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt

AU - Lopes, Bruno Silvester

AU - Al-Agamy, Mohamed H

AU - Ismail, Muhammad A

AU - Shibl, Atef M

AU - Al-Qahtani, Ahmed A

AU - Al-Ahdal, Mohammed N

AU - Forbes, Ken J

N1 - Copyright © 2015 Elsevier GmbH. All rights reserved. Acknowledgements We are grateful for the BMedSc project grant to BSL and MAI by the University of Aberdeen that funded this work and to Murray Robb for helpful comments. MHAA and AMS would also like to extend their appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-VPP-038.

PY - 2015/9

Y1 - 2015/9

N2 - Carbapenem-resistant Acinetobacter spp. have been increasingly reported worldwide including Saudi Arabia and Egypt. We examined 64, non-repetitive, Acinetobacter baumannii isolates collected in 2013 and 2014 from four different medical centres (two from Saudi Arabia and two from Egypt). All the isolates were resistant to ceftazidime and ciprofloxacin. The intI1 harbouring blaGES-11 and aac-6'-1b was detected in 19% (n=12) of the isolates. ISAba1 over-expression of blaADC gene was observed in 65% (n=42) of isolates. Of all the isolates 19% (n=12) had ISAba1 upstream of the blaOXA-51-like gene, 69% (n=44) carried the blaOXA-23 gene within the Tn2006 structure, 8% (n=5) had blaOXA-24-like gene and 9% (n=6) harboured either blaVIM-2 or blaNDM-1 gene. Eighty nine percent (n=57) of isolates were resistant to imipenem and had an MIC of ≥8mg/L. Pulsed-field gel electrophoresis (PFGE) typing revealed the presence of 23 different PFGE. Three PFGE types were very widespread, ST236 (CC104) (PFGE type 1, n=15), ST208 (CC92) (PFGE type 2, n=10), ST884 (CC unassigned) (PFGE type 3, n=7) in and across all four medical centres. The blaOXA-23 gene was found to be present on a 60kb transferable plasmid in both PFGE type 1 and 2 but was absent in PFGE type 3. This is the first study to report on the emergence of ST236 in Saudi Arabia and Egypt, and spread of distinct carbapenem resistant A. baumannii clones belonging to ST884, ST945 and ST1096 in Saudi Arabia.

AB - Carbapenem-resistant Acinetobacter spp. have been increasingly reported worldwide including Saudi Arabia and Egypt. We examined 64, non-repetitive, Acinetobacter baumannii isolates collected in 2013 and 2014 from four different medical centres (two from Saudi Arabia and two from Egypt). All the isolates were resistant to ceftazidime and ciprofloxacin. The intI1 harbouring blaGES-11 and aac-6'-1b was detected in 19% (n=12) of the isolates. ISAba1 over-expression of blaADC gene was observed in 65% (n=42) of isolates. Of all the isolates 19% (n=12) had ISAba1 upstream of the blaOXA-51-like gene, 69% (n=44) carried the blaOXA-23 gene within the Tn2006 structure, 8% (n=5) had blaOXA-24-like gene and 9% (n=6) harboured either blaVIM-2 or blaNDM-1 gene. Eighty nine percent (n=57) of isolates were resistant to imipenem and had an MIC of ≥8mg/L. Pulsed-field gel electrophoresis (PFGE) typing revealed the presence of 23 different PFGE. Three PFGE types were very widespread, ST236 (CC104) (PFGE type 1, n=15), ST208 (CC92) (PFGE type 2, n=10), ST884 (CC unassigned) (PFGE type 3, n=7) in and across all four medical centres. The blaOXA-23 gene was found to be present on a 60kb transferable plasmid in both PFGE type 1 and 2 but was absent in PFGE type 3. This is the first study to report on the emergence of ST236 in Saudi Arabia and Egypt, and spread of distinct carbapenem resistant A. baumannii clones belonging to ST884, ST945 and ST1096 in Saudi Arabia.

KW - Acinetobacter baumannii

KW - carbapenems

KW - Middle-East

KW - OXA-23

KW - plasmid

U2 - 10.1016/j.ijmm.2015.07.007

DO - 10.1016/j.ijmm.2015.07.007

M3 - Article

C2 - 26253451

SN - 1438-4221

VL - 305

SP - 581

EP - 588

JO - International Journal of Medical Microbiology: IJMM

JF - International Journal of Medical Microbiology: IJMM

IS - 6

ER -

The transferability of blaOXA-23 gene in multidrug-resistant Acinetobacter baumannii isolates from Saudi Arabia and Egypt

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this