The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair

Laura Manelyte, Colin P Guy, Rachel M Smith, Mark S Dillingham, Peter McGlynn, Nigel J Savery

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

During nucleotide excision repair (NER) in bacteria the UvrC nuclease and the short oligonucleotide that contains the DNA lesion are removed from the post-incision complex by UvrD, a superfamily 1A helicase. Helicases are frequently regulated by interactions with partner proteins, and immunoprecipitation experiments have previously indicated that UvrD interacts with UvrB, a component of the post-incision complex. We examined this interaction using 2-hybrid analysis and surface plasmon resonance spectroscopy, and found that the N-terminal domain and the unstructured region at the C-terminus of UvrD interact with UvrB. We analysed the properties of a truncated UvrD protein that lacked the unstructured C-terminal region and found that it showed a diminished affinity for single-stranded DNA, but retained the ability to displace both UvrC and the lesion-containing of oligonucleotide from a post-incision nucleotide excision repair complex. The interaction of the C-terminal region of UvrD with UvrB is therefore not an essential feature of the mechanism by which UvrD disassembles the post-incision complex during NER. In further experiments we showed that PcrA helicase from Bacillus stearothermophilus can also displace UvrC and the excised oligonucleotide from a post-incision NER complex, which supports the idea that PcrA performs a UvrD-like function during NER in Gram-positive organisms. (C) 2009 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)1300-1310
Number of pages11
JournalDNA Repair
Volume8
Issue number11
Early online date16 Sept 2009
DOIs
Publication statusPublished - 2 Nov 2009

Keywords

  • nucleotide excision repair
  • helicase recruitment
  • protein-protein interactions
  • DNA helicase-II
  • stearothermophillus PCRA helicase
  • polymerase alpha-subunit
  • coli RNA-polymer
  • escherichia-coli
  • mismatch repair
  • physical interaction
  • crystal-structure
  • protein
  • binding

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