The use of BeWo cells as an in vitro model for placental iron transport

Sarah J Heaton, John J Eady, Mary L Parker, Kathryn L Gotts, Jack R Dainty, Susan J Fairweather-Tait, Harry J McArdle, Kaila S Srai, Ruan M Elliott

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

BeWo cells are a placental cell line that has been widely used as an in vitro model for the placenta. The b30 subclone of these cells can be grown on permeable membranes in bicameral chambers to form confluent cell layers, enabling rates of both nutrient uptake into the cells from the apical surface and efflux from the basolateral membrane to be determined. The aim of this study was to evaluate structural and functional properties of confluent b30 BeWo cell layers grown in bicameral chambers, focusing on the potential application for studying receptor-mediated uptake and transport of transferrin (Tf)-bound iron (Fe-Tf). While it proved extremely difficult to establish and maintain an intact BeWo cell monolayer, it was possible to grow the cells to a confluent multilayer. Iron, applied as Fe-Tf, was rapidly transported across this cell layer; 9.3 +/- 0.5% of the total dose was transported after 8 h, equivalent to 38.8 +/- 2.1 pmol.cm(-2).h(-1). Transfer of Tf across the cell layer was much more limited; 2.4 +/- 0.2% of the total dose was transported after 8 h, equivalent to 5.0 +/- 0.4 pmol.cm(-2).h(-1). Compartmental modeling of these data suggested that iron was transported across the cell layer predominantly, if not exclusively, via a transcellular route, whereas Tf taken up into the cells was predominantly recycled back to the apical compartment. The results suggest that these cells are very efficient at transporting iron and, under carefully controlled conditions, can be a valuable tool for the study of iron transport in the placenta.
Original languageEnglish
Pages (from-to)C1445-C1453
Number of pages9
JournalAmerican Journal of Physiology: Cell Physiology
Volume295
Issue number5
DOIs
Publication statusPublished - 1 Nov 2008

Fingerprint

Iron
Transferrin
Placenta
In Vitro Techniques
Membranes
Cell Line

Keywords

  • cell culture techniques
  • cell line, tumor
  • cell proliferation
  • diffusion
  • female
  • humans
  • iron
  • kinetics
  • models, biological
  • permeability
  • placenta
  • pregnancy
  • receptors, transferrin
  • transferrin

Cite this

Heaton, S. J., Eady, J. J., Parker, M. L., Gotts, K. L., Dainty, J. R., Fairweather-Tait, S. J., ... Elliott, R. M. (2008). The use of BeWo cells as an in vitro model for placental iron transport. American Journal of Physiology: Cell Physiology, 295(5), C1445-C1453. https://doi.org/10.1152/ajpcell.00286.2008

The use of BeWo cells as an in vitro model for placental iron transport. / Heaton, Sarah J; Eady, John J; Parker, Mary L; Gotts, Kathryn L; Dainty, Jack R; Fairweather-Tait, Susan J; McArdle, Harry J; Srai, Kaila S; Elliott, Ruan M.

In: American Journal of Physiology: Cell Physiology, Vol. 295, No. 5, 01.11.2008, p. C1445-C1453.

Research output: Contribution to journalArticle

Heaton, SJ, Eady, JJ, Parker, ML, Gotts, KL, Dainty, JR, Fairweather-Tait, SJ, McArdle, HJ, Srai, KS & Elliott, RM 2008, 'The use of BeWo cells as an in vitro model for placental iron transport', American Journal of Physiology: Cell Physiology, vol. 295, no. 5, pp. C1445-C1453. https://doi.org/10.1152/ajpcell.00286.2008
Heaton SJ, Eady JJ, Parker ML, Gotts KL, Dainty JR, Fairweather-Tait SJ et al. The use of BeWo cells as an in vitro model for placental iron transport. American Journal of Physiology: Cell Physiology. 2008 Nov 1;295(5):C1445-C1453. https://doi.org/10.1152/ajpcell.00286.2008
Heaton, Sarah J ; Eady, John J ; Parker, Mary L ; Gotts, Kathryn L ; Dainty, Jack R ; Fairweather-Tait, Susan J ; McArdle, Harry J ; Srai, Kaila S ; Elliott, Ruan M. / The use of BeWo cells as an in vitro model for placental iron transport. In: American Journal of Physiology: Cell Physiology. 2008 ; Vol. 295, No. 5. pp. C1445-C1453.
@article{f80b0244fd844e7d8b0e823226b697e5,
title = "The use of BeWo cells as an in vitro model for placental iron transport",
abstract = "BeWo cells are a placental cell line that has been widely used as an in vitro model for the placenta. The b30 subclone of these cells can be grown on permeable membranes in bicameral chambers to form confluent cell layers, enabling rates of both nutrient uptake into the cells from the apical surface and efflux from the basolateral membrane to be determined. The aim of this study was to evaluate structural and functional properties of confluent b30 BeWo cell layers grown in bicameral chambers, focusing on the potential application for studying receptor-mediated uptake and transport of transferrin (Tf)-bound iron (Fe-Tf). While it proved extremely difficult to establish and maintain an intact BeWo cell monolayer, it was possible to grow the cells to a confluent multilayer. Iron, applied as Fe-Tf, was rapidly transported across this cell layer; 9.3 +/- 0.5{\%} of the total dose was transported after 8 h, equivalent to 38.8 +/- 2.1 pmol.cm(-2).h(-1). Transfer of Tf across the cell layer was much more limited; 2.4 +/- 0.2{\%} of the total dose was transported after 8 h, equivalent to 5.0 +/- 0.4 pmol.cm(-2).h(-1). Compartmental modeling of these data suggested that iron was transported across the cell layer predominantly, if not exclusively, via a transcellular route, whereas Tf taken up into the cells was predominantly recycled back to the apical compartment. The results suggest that these cells are very efficient at transporting iron and, under carefully controlled conditions, can be a valuable tool for the study of iron transport in the placenta.",
keywords = "cell culture techniques, cell line, tumor, cell proliferation, diffusion, female, humans, iron, kinetics, models, biological, permeability, placenta, pregnancy, receptors, transferrin, transferrin",
author = "Heaton, {Sarah J} and Eady, {John J} and Parker, {Mary L} and Gotts, {Kathryn L} and Dainty, {Jack R} and Fairweather-Tait, {Susan J} and McArdle, {Harry J} and Srai, {Kaila S} and Elliott, {Ruan M}",
year = "2008",
month = "11",
day = "1",
doi = "10.1152/ajpcell.00286.2008",
language = "English",
volume = "295",
pages = "C1445--C1453",
journal = "American Journal of Physiology: Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - The use of BeWo cells as an in vitro model for placental iron transport

AU - Heaton, Sarah J

AU - Eady, John J

AU - Parker, Mary L

AU - Gotts, Kathryn L

AU - Dainty, Jack R

AU - Fairweather-Tait, Susan J

AU - McArdle, Harry J

AU - Srai, Kaila S

AU - Elliott, Ruan M

PY - 2008/11/1

Y1 - 2008/11/1

N2 - BeWo cells are a placental cell line that has been widely used as an in vitro model for the placenta. The b30 subclone of these cells can be grown on permeable membranes in bicameral chambers to form confluent cell layers, enabling rates of both nutrient uptake into the cells from the apical surface and efflux from the basolateral membrane to be determined. The aim of this study was to evaluate structural and functional properties of confluent b30 BeWo cell layers grown in bicameral chambers, focusing on the potential application for studying receptor-mediated uptake and transport of transferrin (Tf)-bound iron (Fe-Tf). While it proved extremely difficult to establish and maintain an intact BeWo cell monolayer, it was possible to grow the cells to a confluent multilayer. Iron, applied as Fe-Tf, was rapidly transported across this cell layer; 9.3 +/- 0.5% of the total dose was transported after 8 h, equivalent to 38.8 +/- 2.1 pmol.cm(-2).h(-1). Transfer of Tf across the cell layer was much more limited; 2.4 +/- 0.2% of the total dose was transported after 8 h, equivalent to 5.0 +/- 0.4 pmol.cm(-2).h(-1). Compartmental modeling of these data suggested that iron was transported across the cell layer predominantly, if not exclusively, via a transcellular route, whereas Tf taken up into the cells was predominantly recycled back to the apical compartment. The results suggest that these cells are very efficient at transporting iron and, under carefully controlled conditions, can be a valuable tool for the study of iron transport in the placenta.

AB - BeWo cells are a placental cell line that has been widely used as an in vitro model for the placenta. The b30 subclone of these cells can be grown on permeable membranes in bicameral chambers to form confluent cell layers, enabling rates of both nutrient uptake into the cells from the apical surface and efflux from the basolateral membrane to be determined. The aim of this study was to evaluate structural and functional properties of confluent b30 BeWo cell layers grown in bicameral chambers, focusing on the potential application for studying receptor-mediated uptake and transport of transferrin (Tf)-bound iron (Fe-Tf). While it proved extremely difficult to establish and maintain an intact BeWo cell monolayer, it was possible to grow the cells to a confluent multilayer. Iron, applied as Fe-Tf, was rapidly transported across this cell layer; 9.3 +/- 0.5% of the total dose was transported after 8 h, equivalent to 38.8 +/- 2.1 pmol.cm(-2).h(-1). Transfer of Tf across the cell layer was much more limited; 2.4 +/- 0.2% of the total dose was transported after 8 h, equivalent to 5.0 +/- 0.4 pmol.cm(-2).h(-1). Compartmental modeling of these data suggested that iron was transported across the cell layer predominantly, if not exclusively, via a transcellular route, whereas Tf taken up into the cells was predominantly recycled back to the apical compartment. The results suggest that these cells are very efficient at transporting iron and, under carefully controlled conditions, can be a valuable tool for the study of iron transport in the placenta.

KW - cell culture techniques

KW - cell line, tumor

KW - cell proliferation

KW - diffusion

KW - female

KW - humans

KW - iron

KW - kinetics

KW - models, biological

KW - permeability

KW - placenta

KW - pregnancy

KW - receptors, transferrin

KW - transferrin

U2 - 10.1152/ajpcell.00286.2008

DO - 10.1152/ajpcell.00286.2008

M3 - Article

VL - 295

SP - C1445-C1453

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

SN - 0363-6143

IS - 5

ER -