Abstract
This paper presents the performance of electronic holography for imaging applications of biological and medical interest involving turbid media. It demonstrates that digital holography is useful for biological studies in the areas of estuary sediment erosion and imaging unstained living cells. In particular, we demonstrate the recording geometries of classical 'in-line' back illumination using a collimated beam for measuring micro-panicles eroded in a cylindrical observation chamber; we also demonstrate a phase contrast set-up using a magnifying lens for observing biomedical micro-objects of unstained living cells in physiological saline. Practical results are presented for the studies of sediment erosion and transport, which provide information about how polymeric substances produced by the organisms in sediment and erosion shear velocity affect the sediment particle size when erosive stresses are applied to sediment beds.
| Original language | English |
|---|---|
| Pages (from-to) | S399-S407 |
| Number of pages | 10 |
| Journal | Journal of Optics A: Pure and Applied Optics |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- digital/electronic holography
- optical observation chamber
- sediment erosion
- image processing
- data analysis
- hydrodynamic shear velocity
- phase contrast microscopy
- in-line holography
- digital holography
- reconstruction
- sediments
Cite this
The use of digital/electronic holography for biological applications. / Sun, Hongyue; Player, Michael Antony; Watson, John; Hendry, David C.; Perkins, R.; Gust, G.; Paterson, D. M.
In: Journal of Optics A: Pure and Applied Optics, Vol. 7, No. 6, 2005, p. S399-S407.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The use of digital/electronic holography for biological applications
AU - Sun, Hongyue
AU - Player, Michael Antony
AU - Watson, John
AU - Hendry, David C.
AU - Perkins, R.
AU - Gust, G.
AU - Paterson, D. M.
PY - 2005
Y1 - 2005
N2 - This paper presents the performance of electronic holography for imaging applications of biological and medical interest involving turbid media. It demonstrates that digital holography is useful for biological studies in the areas of estuary sediment erosion and imaging unstained living cells. In particular, we demonstrate the recording geometries of classical 'in-line' back illumination using a collimated beam for measuring micro-panicles eroded in a cylindrical observation chamber; we also demonstrate a phase contrast set-up using a magnifying lens for observing biomedical micro-objects of unstained living cells in physiological saline. Practical results are presented for the studies of sediment erosion and transport, which provide information about how polymeric substances produced by the organisms in sediment and erosion shear velocity affect the sediment particle size when erosive stresses are applied to sediment beds.
AB - This paper presents the performance of electronic holography for imaging applications of biological and medical interest involving turbid media. It demonstrates that digital holography is useful for biological studies in the areas of estuary sediment erosion and imaging unstained living cells. In particular, we demonstrate the recording geometries of classical 'in-line' back illumination using a collimated beam for measuring micro-panicles eroded in a cylindrical observation chamber; we also demonstrate a phase contrast set-up using a magnifying lens for observing biomedical micro-objects of unstained living cells in physiological saline. Practical results are presented for the studies of sediment erosion and transport, which provide information about how polymeric substances produced by the organisms in sediment and erosion shear velocity affect the sediment particle size when erosive stresses are applied to sediment beds.
KW - digital/electronic holography
KW - optical observation chamber
KW - sediment erosion
KW - image processing
KW - data analysis
KW - hydrodynamic shear velocity
KW - phase contrast microscopy
KW - in-line holography
KW - digital holography
KW - reconstruction
KW - sediments
U2 - 10.1088/1464-4258/7/6/022
DO - 10.1088/1464-4258/7/6/022
M3 - Article
VL - 7
SP - S399-S407
JO - Journal of Optics A: Pure and Applied Optics
JF - Journal of Optics A: Pure and Applied Optics
SN - 1464-4258
IS - 6
ER -