The use of digital/electronic holography for biological applications

Hongyue Sun, Michael Antony Player, John Watson, David C. Hendry, R. Perkins, G. Gust, D. M. Paterson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This paper presents the performance of electronic holography for imaging applications of biological and medical interest involving turbid media. It demonstrates that digital holography is useful for biological studies in the areas of estuary sediment erosion and imaging unstained living cells. In particular, we demonstrate the recording geometries of classical 'in-line' back illumination using a collimated beam for measuring micro-panicles eroded in a cylindrical observation chamber; we also demonstrate a phase contrast set-up using a magnifying lens for observing biomedical micro-objects of unstained living cells in physiological saline. Practical results are presented for the studies of sediment erosion and transport, which provide information about how polymeric substances produced by the organisms in sediment and erosion shear velocity affect the sediment particle size when erosive stresses are applied to sediment beds.

Original languageEnglish
Pages (from-to)S399-S407
Number of pages10
JournalJournal of Optics A: Pure and Applied Optics
Volume7
Issue number6
DOIs
Publication statusPublished - 2005

Keywords

  • digital/electronic holography
  • optical observation chamber
  • sediment erosion
  • image processing
  • data analysis
  • hydrodynamic shear velocity
  • phase contrast microscopy
  • in-line holography
  • digital holography
  • reconstruction
  • sediments

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