The use of DNA fingerprinting to assess monozygotic twinning in Meishan and Landrace x Large White pigs

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6 Citations (Scopus)

Abstract

The extent of embryo mortality is usually estimated by the difference between the numbers of corpora lutea and embryos in the same animal, which assumes that each corpora lutea represents one potential embryo. Recent observations from the authors' laboratory reveal situations in which the number of embryos exceeds the number of corpora lutea, implicating the presence of identical twins. The objective of the present study was to establish DNA fingerprinting techniques to investigate the prevalence of monozygotic twinning in two breeds of pig. DNA fingerprints of every fetus carried by 6 Meishan (MS) and 6 Landrace x Large White (LxLW) gilts on Day 29 +/- 2 of pregnancy were obtained. Five LxLW and 3 MS litters carried no identical fetuses. The remaining gilts carried a pair of fetuses with indistinguishable DNA profiles. No pairs of fetuses were monochorionic. These results suggest that monozygotic twinning in the pig occurs during embryo cleavage or blastocyst development and may be more prevalent in MS compared with indigenous breeds.

Original languageEnglish
Pages (from-to)487-490
Number of pages4
JournalReproduction, Fertility and Development
Volume10
Issue number6
DOIs
Publication statusPublished - 1998

Keywords

  • embryo survival
  • conceptus growth
  • gilts

Cite this

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title = "The use of DNA fingerprinting to assess monozygotic twinning in Meishan and Landrace x Large White pigs",
abstract = "The extent of embryo mortality is usually estimated by the difference between the numbers of corpora lutea and embryos in the same animal, which assumes that each corpora lutea represents one potential embryo. Recent observations from the authors' laboratory reveal situations in which the number of embryos exceeds the number of corpora lutea, implicating the presence of identical twins. The objective of the present study was to establish DNA fingerprinting techniques to investigate the prevalence of monozygotic twinning in two breeds of pig. DNA fingerprints of every fetus carried by 6 Meishan (MS) and 6 Landrace x Large White (LxLW) gilts on Day 29 +/- 2 of pregnancy were obtained. Five LxLW and 3 MS litters carried no identical fetuses. The remaining gilts carried a pair of fetuses with indistinguishable DNA profiles. No pairs of fetuses were monochorionic. These results suggest that monozygotic twinning in the pig occurs during embryo cleavage or blastocyst development and may be more prevalent in MS compared with indigenous breeds.",
keywords = "embryo survival, conceptus growth, gilts",
author = "Ashworth, {C J} and Alexander Ross and Perry Barrett",
year = "1998",
doi = "10.1071/RD99010",
language = "English",
volume = "10",
pages = "487--490",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "6",

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TY - JOUR

T1 - The use of DNA fingerprinting to assess monozygotic twinning in Meishan and Landrace x Large White pigs

AU - Ashworth, C J

AU - Ross, Alexander

AU - Barrett, Perry

PY - 1998

Y1 - 1998

N2 - The extent of embryo mortality is usually estimated by the difference between the numbers of corpora lutea and embryos in the same animal, which assumes that each corpora lutea represents one potential embryo. Recent observations from the authors' laboratory reveal situations in which the number of embryos exceeds the number of corpora lutea, implicating the presence of identical twins. The objective of the present study was to establish DNA fingerprinting techniques to investigate the prevalence of monozygotic twinning in two breeds of pig. DNA fingerprints of every fetus carried by 6 Meishan (MS) and 6 Landrace x Large White (LxLW) gilts on Day 29 +/- 2 of pregnancy were obtained. Five LxLW and 3 MS litters carried no identical fetuses. The remaining gilts carried a pair of fetuses with indistinguishable DNA profiles. No pairs of fetuses were monochorionic. These results suggest that monozygotic twinning in the pig occurs during embryo cleavage or blastocyst development and may be more prevalent in MS compared with indigenous breeds.

AB - The extent of embryo mortality is usually estimated by the difference between the numbers of corpora lutea and embryos in the same animal, which assumes that each corpora lutea represents one potential embryo. Recent observations from the authors' laboratory reveal situations in which the number of embryos exceeds the number of corpora lutea, implicating the presence of identical twins. The objective of the present study was to establish DNA fingerprinting techniques to investigate the prevalence of monozygotic twinning in two breeds of pig. DNA fingerprints of every fetus carried by 6 Meishan (MS) and 6 Landrace x Large White (LxLW) gilts on Day 29 +/- 2 of pregnancy were obtained. Five LxLW and 3 MS litters carried no identical fetuses. The remaining gilts carried a pair of fetuses with indistinguishable DNA profiles. No pairs of fetuses were monochorionic. These results suggest that monozygotic twinning in the pig occurs during embryo cleavage or blastocyst development and may be more prevalent in MS compared with indigenous breeds.

KW - embryo survival

KW - conceptus growth

KW - gilts

U2 - 10.1071/RD99010

DO - 10.1071/RD99010

M3 - Article

VL - 10

SP - 487

EP - 490

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 6

ER -