The vascularly and luminally perfused small intestine in vitro: dissection technique and model system

J D Hutchison, V J Undrill, J W Porteous

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The quantitative study in vitro of a wide range of intestinal functions is best achieved by perfusing the intestine simultaneously by the luminal and vascular routes. A comprehensive description is given of the dissections involved in preparing a selected segment of jejunum for such simultaneous perfusions. Attention is drawn to a number of occasional aberrant vascular structures which require special attention if successful perfusions are to be established. It is essential to restore the ATP and diphosphoglycerate concentrations of erythrocytes, and to assess their deformability, before incorporating them in the vascular perfusion medium. The perfusion equipment, the perfusion media and the general conduct of a perfusion experiment, are described.
Original languageEnglish
Pages (from-to)168-183
Number of pages16
JournalLaboratory Animals
Volume25
Issue number2
DOIs
Publication statusPublished - Apr 1991

Fingerprint

blood vessels
Small Intestine
small intestine
Dissection
Perfusion
jejunum
in vitro studies
Blood Vessels
intestines
erythrocytes
methodology
Tunica Media
Jejunum
Intestines
In Vitro Techniques
Adenosine Triphosphate
Erythrocytes
Equipment and Supplies

Keywords

  • Anesthesia
  • Animals
  • Dissection
  • Erythrocyte Deformability
  • Glycolysis
  • Intestine, Small
  • Muscle, Smooth
  • Perfusion
  • Rats

Cite this

The vascularly and luminally perfused small intestine in vitro : dissection technique and model system. / Hutchison, J D; Undrill, V J; Porteous, J W.

In: Laboratory Animals, Vol. 25, No. 2, 04.1991, p. 168-183.

Research output: Contribution to journalArticle

Hutchison, J D ; Undrill, V J ; Porteous, J W. / The vascularly and luminally perfused small intestine in vitro : dissection technique and model system. In: Laboratory Animals. 1991 ; Vol. 25, No. 2. pp. 168-183.
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