Abstract
In recent years, we have used a variety of tau immunological markers combined with the dye thiazin red (TR), an accurate marker to differentiate the fibrillar from the nonfibrillar state of both amyloid-beta and tau in Alzheimer's disease (AD). In this study, we used TR as a potential diagnostic marker of AD in frozen-thawed (F-T) brain tissue and imprint cytology. Control experiments included the use of Thioflavin-S staining, fixed tissue, and some double-labeled material with TR and selected tau markers, including AT100, MC1, Alz-50, TG-3, Tau-C3, and S396. Our results indicate that TR retains its strong affinity for both tangles and plaques in unfixed F-T tissue and imprint cytology. This information provides a potential use of TR as an accurate diagnostic tool for the rapid postmortem diagnosis of AD neuropathology. This study shows the advantages of TR on cytology mainly because tools for the fast postmortem diagnosis of AD are practically nonexistent. In addition, we observed Tau-C3 immunoreactivity in extracellular tangles, suggesting that the Tau-C3 epitope is characteristically stable. Moreover, this study demonstrates that chemical fixation is not necessarily required for tau immunoreactivity on histological sections.
| Original language | English |
|---|---|
| Pages (from-to) | 507-515 |
| Number of pages | 9 |
| Journal | Acta Neuropathologica |
| Volume | 116 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2008 |
Keywords
- touch imprint
- neuropathology
- thiazin red
- neurofibrillary tangle
- immunohistochemistry
- Tau protein
- Alzheimer's disease
- paired helical filaments
- neurofibrillary tangles
- TAU-protein
- antibody
- phosphorylation
- pathology
- neurons
- gallyas
- glycosylation
- conformation
Cite this
Thiazin red as a neuropathological tool for the rapid diagnosis of Alzheimer's disease in tissue imprints. / Luna-Munoz, Jose; Peralta-Ramirez, Janneth; Chavez-Macias, Laura; Harrington, Charles R.; Wischik, Claude M.; Mena, Raul.
In: Acta Neuropathologica, Vol. 116, No. 5, 11.2008, p. 507-515.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thiazin red as a neuropathological tool for the rapid diagnosis of Alzheimer's disease in tissue imprints
AU - Luna-Munoz, Jose
AU - Peralta-Ramirez, Janneth
AU - Chavez-Macias, Laura
AU - Harrington, Charles R.
AU - Wischik, Claude M.
AU - Mena, Raul
PY - 2008/11
Y1 - 2008/11
N2 - In recent years, we have used a variety of tau immunological markers combined with the dye thiazin red (TR), an accurate marker to differentiate the fibrillar from the nonfibrillar state of both amyloid-beta and tau in Alzheimer's disease (AD). In this study, we used TR as a potential diagnostic marker of AD in frozen-thawed (F-T) brain tissue and imprint cytology. Control experiments included the use of Thioflavin-S staining, fixed tissue, and some double-labeled material with TR and selected tau markers, including AT100, MC1, Alz-50, TG-3, Tau-C3, and S396. Our results indicate that TR retains its strong affinity for both tangles and plaques in unfixed F-T tissue and imprint cytology. This information provides a potential use of TR as an accurate diagnostic tool for the rapid postmortem diagnosis of AD neuropathology. This study shows the advantages of TR on cytology mainly because tools for the fast postmortem diagnosis of AD are practically nonexistent. In addition, we observed Tau-C3 immunoreactivity in extracellular tangles, suggesting that the Tau-C3 epitope is characteristically stable. Moreover, this study demonstrates that chemical fixation is not necessarily required for tau immunoreactivity on histological sections.
AB - In recent years, we have used a variety of tau immunological markers combined with the dye thiazin red (TR), an accurate marker to differentiate the fibrillar from the nonfibrillar state of both amyloid-beta and tau in Alzheimer's disease (AD). In this study, we used TR as a potential diagnostic marker of AD in frozen-thawed (F-T) brain tissue and imprint cytology. Control experiments included the use of Thioflavin-S staining, fixed tissue, and some double-labeled material with TR and selected tau markers, including AT100, MC1, Alz-50, TG-3, Tau-C3, and S396. Our results indicate that TR retains its strong affinity for both tangles and plaques in unfixed F-T tissue and imprint cytology. This information provides a potential use of TR as an accurate diagnostic tool for the rapid postmortem diagnosis of AD neuropathology. This study shows the advantages of TR on cytology mainly because tools for the fast postmortem diagnosis of AD are practically nonexistent. In addition, we observed Tau-C3 immunoreactivity in extracellular tangles, suggesting that the Tau-C3 epitope is characteristically stable. Moreover, this study demonstrates that chemical fixation is not necessarily required for tau immunoreactivity on histological sections.
KW - touch imprint
KW - neuropathology
KW - thiazin red
KW - neurofibrillary tangle
KW - immunohistochemistry
KW - Tau protein
KW - Alzheimer's disease
KW - paired helical filaments
KW - neurofibrillary tangles
KW - TAU-protein
KW - antibody
KW - phosphorylation
KW - pathology
KW - neurons
KW - gallyas
KW - glycosylation
KW - conformation
U2 - 10.1007/s00401-008-0431-x
DO - 10.1007/s00401-008-0431-x
M3 - Article
VL - 116
SP - 507
EP - 515
JO - Acta Neuropathologica
JF - Acta Neuropathologica
SN - 0001-6322
IS - 5
ER -