Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster

S Messager, M L Garabette, M H Hastings, D G Hazlerigg

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Melatonin secretion by the pineal gland transduces photoperiod into a neuroendocrine signal. In the pars tuberalis (PT), we have shown that photoperiod modifies the amplitude of the clock gene Perl. The aim of this study was to test whether the endogenous melatonin signal is required for rhythmic expression of Perl in the PT. Male Syrian hamsters housed in long days (LD, 16:8 h light:dark) were pinealectomized and Perl mRNA expression studied by in situ hybridization. Pinealectomy abolished the rhythm of Perl expression in the PT, but had no effect on Perl expression in the suprachiasmatic nucleus (SCN), or the ventromedial nucleus (VMH) of the hypothalamus. Interestingly, a single night-time injection of melatonin (25 mug) given to pinealectomized animals, failed to restore Perl expression in the PT. These data demonstrate that Perl expression in the PT is driven by melatonin, and that the features of the endogenous signal through which the Perl expression is achieved cannot be reproduced by a single melatonin injection. NeuroReport 12:579-582 (C) 2001 Lippincott Williams & Wilkins.

Original languageEnglish
Pages (from-to)579-582
Number of pages4
JournalNeuroreport
Volume12
Publication statusPublished - 2001

Keywords

  • circadian rhythms
  • clock gene
  • melatonin
  • pars tuberalis
  • Per1
  • photoperiodism
  • suprachiasmatic nucleus
  • Syrian hamster
  • IODOMELATONIN-BINDING-SITES
  • SUPRACHIASMATIC NUCLEUS
  • SIBERIAN HAMSTER
  • CIRCADIAN CLOCK
  • PHOTOPERIODIC REGULATION
  • MELATONIN
  • TIME
  • GENE
  • ICER

Cite this

Messager, S., Garabette, M. L., Hastings, M. H., & Hazlerigg, D. G. (2001). Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster. Neuroreport, 12, 579-582.

Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster. / Messager, S ; Garabette, M L ; Hastings, M H ; Hazlerigg, D G .

In: Neuroreport, Vol. 12, 2001, p. 579-582.

Research output: Contribution to journalArticle

Messager, S, Garabette, ML, Hastings, MH & Hazlerigg, DG 2001, 'Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster', Neuroreport, vol. 12, pp. 579-582.
Messager, S ; Garabette, M L ; Hastings, M H ; Hazlerigg, D G . / Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster. In: Neuroreport. 2001 ; Vol. 12. pp. 579-582.
@article{17b69ed121cf4c19900c4ae49e02a17e,
title = "Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster",
abstract = "Melatonin secretion by the pineal gland transduces photoperiod into a neuroendocrine signal. In the pars tuberalis (PT), we have shown that photoperiod modifies the amplitude of the clock gene Perl. The aim of this study was to test whether the endogenous melatonin signal is required for rhythmic expression of Perl in the PT. Male Syrian hamsters housed in long days (LD, 16:8 h light:dark) were pinealectomized and Perl mRNA expression studied by in situ hybridization. Pinealectomy abolished the rhythm of Perl expression in the PT, but had no effect on Perl expression in the suprachiasmatic nucleus (SCN), or the ventromedial nucleus (VMH) of the hypothalamus. Interestingly, a single night-time injection of melatonin (25 mug) given to pinealectomized animals, failed to restore Perl expression in the PT. These data demonstrate that Perl expression in the PT is driven by melatonin, and that the features of the endogenous signal through which the Perl expression is achieved cannot be reproduced by a single melatonin injection. NeuroReport 12:579-582 (C) 2001 Lippincott Williams & Wilkins.",
keywords = "circadian rhythms, clock gene, melatonin, pars tuberalis, Per1, photoperiodism, suprachiasmatic nucleus, Syrian hamster, IODOMELATONIN-BINDING-SITES, SUPRACHIASMATIC NUCLEUS, SIBERIAN HAMSTER, CIRCADIAN CLOCK, PHOTOPERIODIC REGULATION, MELATONIN, TIME, GENE, ICER",
author = "S Messager and Garabette, {M L} and Hastings, {M H} and Hazlerigg, {D G}",
year = "2001",
language = "English",
volume = "12",
pages = "579--582",
journal = "Neuroreport",
issn = "0959-4965",
publisher = "LWW",

}

TY - JOUR

T1 - Tissue-specific abolition of Per1 expression in the pars tuberalis by pinealectomy in the Syrian hamster

AU - Messager, S

AU - Garabette, M L

AU - Hastings, M H

AU - Hazlerigg, D G

PY - 2001

Y1 - 2001

N2 - Melatonin secretion by the pineal gland transduces photoperiod into a neuroendocrine signal. In the pars tuberalis (PT), we have shown that photoperiod modifies the amplitude of the clock gene Perl. The aim of this study was to test whether the endogenous melatonin signal is required for rhythmic expression of Perl in the PT. Male Syrian hamsters housed in long days (LD, 16:8 h light:dark) were pinealectomized and Perl mRNA expression studied by in situ hybridization. Pinealectomy abolished the rhythm of Perl expression in the PT, but had no effect on Perl expression in the suprachiasmatic nucleus (SCN), or the ventromedial nucleus (VMH) of the hypothalamus. Interestingly, a single night-time injection of melatonin (25 mug) given to pinealectomized animals, failed to restore Perl expression in the PT. These data demonstrate that Perl expression in the PT is driven by melatonin, and that the features of the endogenous signal through which the Perl expression is achieved cannot be reproduced by a single melatonin injection. NeuroReport 12:579-582 (C) 2001 Lippincott Williams & Wilkins.

AB - Melatonin secretion by the pineal gland transduces photoperiod into a neuroendocrine signal. In the pars tuberalis (PT), we have shown that photoperiod modifies the amplitude of the clock gene Perl. The aim of this study was to test whether the endogenous melatonin signal is required for rhythmic expression of Perl in the PT. Male Syrian hamsters housed in long days (LD, 16:8 h light:dark) were pinealectomized and Perl mRNA expression studied by in situ hybridization. Pinealectomy abolished the rhythm of Perl expression in the PT, but had no effect on Perl expression in the suprachiasmatic nucleus (SCN), or the ventromedial nucleus (VMH) of the hypothalamus. Interestingly, a single night-time injection of melatonin (25 mug) given to pinealectomized animals, failed to restore Perl expression in the PT. These data demonstrate that Perl expression in the PT is driven by melatonin, and that the features of the endogenous signal through which the Perl expression is achieved cannot be reproduced by a single melatonin injection. NeuroReport 12:579-582 (C) 2001 Lippincott Williams & Wilkins.

KW - circadian rhythms

KW - clock gene

KW - melatonin

KW - pars tuberalis

KW - Per1

KW - photoperiodism

KW - suprachiasmatic nucleus

KW - Syrian hamster

KW - IODOMELATONIN-BINDING-SITES

KW - SUPRACHIASMATIC NUCLEUS

KW - SIBERIAN HAMSTER

KW - CIRCADIAN CLOCK

KW - PHOTOPERIODIC REGULATION

KW - MELATONIN

KW - TIME

KW - GENE

KW - ICER

M3 - Article

VL - 12

SP - 579

EP - 582

JO - Neuroreport

JF - Neuroreport

SN - 0959-4965

ER -