Tissue-specific changes in RNA synthesis in vivo during anoxia in crucian carp

R W Smith, D F Houlihan, G E Nilsson, J Alexandre

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with RNA synthesis accounting for the former and protein synthesis the latter. During anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. The present study examines RNA synthesis during anoxia by labeling with [H-3]uridine. A novel synthesis rate calculation is presented, which allows for the tissue-specific salvage of uridine, with synthesis rates finally expressed relative to DNA. After 48 h anoxia, the decline (29%) in brain RNA synthesis and increases in the heart and liver (132 and 871%, respectively) support known RNA functions during hypoxic/anoxic survival. This study provides evidence that, in an anoxia-tolerant species, survival mechanisms involving RNA are able to operate because tissue-specific restructuring of the RNA synthesis process enables fixed synthesis costs to be maintained; this may be as vital to survival as exploiting the variable energetic demand of protein synthesis.

Original languageEnglish
Number of pages8
JournalAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiology
Volume277
Publication statusPublished - 1999

Keywords

  • precursor-dependent RNA synthesis
  • nucleotide salvage
  • fixed pretranslational energy cost
  • PROTEIN-SYNTHESIS
  • NUCLEOSIDE TRANSPORT
  • ONCORHYNCHUS-MYKISS
  • URIDINE UPTAKE
  • RAINBOW-TROUT
  • MESSENGER-RNA
  • ANIMAL-CELLS
  • LIVER
  • NUCLEOTIDES
  • ISCHEMIA

Cite this

Tissue-specific changes in RNA synthesis in vivo during anoxia in crucian carp. / Smith, R W ; Houlihan, D F ; Nilsson, G E ; Alexandre, J .

In: American Journal of Physiology-Regulatory Integrative and Comparative Physiology, Vol. 277, 1999.

Research output: Contribution to journalArticle

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T1 - Tissue-specific changes in RNA synthesis in vivo during anoxia in crucian carp

AU - Smith, R W

AU - Houlihan, D F

AU - Nilsson, G E

AU - Alexandre, J

PY - 1999

Y1 - 1999

N2 - The overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with RNA synthesis accounting for the former and protein synthesis the latter. During anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. The present study examines RNA synthesis during anoxia by labeling with [H-3]uridine. A novel synthesis rate calculation is presented, which allows for the tissue-specific salvage of uridine, with synthesis rates finally expressed relative to DNA. After 48 h anoxia, the decline (29%) in brain RNA synthesis and increases in the heart and liver (132 and 871%, respectively) support known RNA functions during hypoxic/anoxic survival. This study provides evidence that, in an anoxia-tolerant species, survival mechanisms involving RNA are able to operate because tissue-specific restructuring of the RNA synthesis process enables fixed synthesis costs to be maintained; this may be as vital to survival as exploiting the variable energetic demand of protein synthesis.

AB - The overall energy budget for protein synthesis (i.e., transcription plus translation) is thought to consist of fixed and variable components, with RNA synthesis accounting for the former and protein synthesis the latter. During anoxia, the downregulation of protein synthesis (i.e., the variable component), to reduce energetic demand, is an important aspect of survival in crucian carp. The present study examines RNA synthesis during anoxia by labeling with [H-3]uridine. A novel synthesis rate calculation is presented, which allows for the tissue-specific salvage of uridine, with synthesis rates finally expressed relative to DNA. After 48 h anoxia, the decline (29%) in brain RNA synthesis and increases in the heart and liver (132 and 871%, respectively) support known RNA functions during hypoxic/anoxic survival. This study provides evidence that, in an anoxia-tolerant species, survival mechanisms involving RNA are able to operate because tissue-specific restructuring of the RNA synthesis process enables fixed synthesis costs to be maintained; this may be as vital to survival as exploiting the variable energetic demand of protein synthesis.

KW - precursor-dependent RNA synthesis

KW - nucleotide salvage

KW - fixed pretranslational energy cost

KW - PROTEIN-SYNTHESIS

KW - NUCLEOSIDE TRANSPORT

KW - ONCORHYNCHUS-MYKISS

KW - URIDINE UPTAKE

KW - RAINBOW-TROUT

KW - MESSENGER-RNA

KW - ANIMAL-CELLS

KW - LIVER

KW - NUCLEOTIDES

KW - ISCHEMIA

M3 - Article

VL - 277

JO - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology-Regulatory Integrative and Comparative Physiology

SN - 0363-6119

ER -