Torsin A protects against oxidative stress in COS-1 and PC12 cells

R. Kuner, Peter Teismann, A. Trutzel, J. Naim, A. Richter, N. Schmidt, O. Von Ahsen, A. Bach, B. Ferger, A. Schneider

Research output: Contribution to journalArticle

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Abstract

Dystonia is a highly frequent movement disorder, the pathogenesis of which remains unclear. The cloning of TorsinA, the gene responsible for early-onset dystonia, was a major breakthrough. However, the function of this protein remains unclear. By sequence homology, TorsinA belongs to the ATPases associated with diverse cellular activities-family, many of whose members are chaperones and/or proteases. We report here that in an in vitro model for oxidative stress, H2O2 treatment, overexpression of TorsinA was protective against cell death. COS-1 cells overexpressing TorsinA demonstrated drastically reduced terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling-staining following exposure to H2O2. Furthermore, transfection with TorsinA significantly increased survival of PC12 after H2O2 treatment. To our knowledge, this is the first demonstration that TorsinA protects against oxidative stress. We speculate that a loss of this cellular function in mutant TorsinA may be linked to the pathogenesis of early-onset dystonia.

Original languageEnglish
Pages (from-to)153-156
Number of pages3
JournalNeuroscience Letters
Volume350
Issue number3
Early online date5 Sep 2003
DOIs
Publication statusPublished - 30 Oct 2003

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Dystonia
COS Cells
PC12 Cells
Oxidative Stress
DNA Nucleotidylexotransferase
Movement Disorders
Biotin
Sequence Homology
Transfection
Organism Cloning
Peptide Hydrolases
Cell Death
Staining and Labeling
Therapeutics
Genes
Proteins

Keywords

  • Dystonia
  • TorsinA
  • Oxidative stress

Cite this

Kuner, R., Teismann, P., Trutzel, A., Naim, J., Richter, A., Schmidt, N., ... Schneider, A. (2003). Torsin A protects against oxidative stress in COS-1 and PC12 cells. Neuroscience Letters, 350(3), 153-156. https://doi.org/10.1016/S0304-3940(03)00904-2

Torsin A protects against oxidative stress in COS-1 and PC12 cells. / Kuner, R.; Teismann, Peter; Trutzel, A.; Naim, J.; Richter, A.; Schmidt, N.; Von Ahsen, O.; Bach, A.; Ferger, B.; Schneider, A.

In: Neuroscience Letters, Vol. 350, No. 3, 30.10.2003, p. 153-156.

Research output: Contribution to journalArticle

Kuner, R, Teismann, P, Trutzel, A, Naim, J, Richter, A, Schmidt, N, Von Ahsen, O, Bach, A, Ferger, B & Schneider, A 2003, 'Torsin A protects against oxidative stress in COS-1 and PC12 cells', Neuroscience Letters, vol. 350, no. 3, pp. 153-156. https://doi.org/10.1016/S0304-3940(03)00904-2
Kuner R, Teismann P, Trutzel A, Naim J, Richter A, Schmidt N et al. Torsin A protects against oxidative stress in COS-1 and PC12 cells. Neuroscience Letters. 2003 Oct 30;350(3):153-156. https://doi.org/10.1016/S0304-3940(03)00904-2
Kuner, R. ; Teismann, Peter ; Trutzel, A. ; Naim, J. ; Richter, A. ; Schmidt, N. ; Von Ahsen, O. ; Bach, A. ; Ferger, B. ; Schneider, A. / Torsin A protects against oxidative stress in COS-1 and PC12 cells. In: Neuroscience Letters. 2003 ; Vol. 350, No. 3. pp. 153-156.
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