Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function

Serena Zacchigna; Alessia Paldino; Inês Falcão-Pires; Evangelos P Daskalopoulos; Matteo Dal Ferro; Simone Vodret; Pierluigi Lesizza; Antonio Cannatà; Daniela Miranda-Silva; André P Lourenço; Bruno Pinamonti; Gianfranco Sinagra; Florian Weinberger; Thomas Eschenhagen; Lucie Carrier; Izhak Kehat; Carlo G Tocchetti; Michele Russo; Alessandra Ghigo; James Cimino; Emilio Hirsch; Dana Dawson; Michele Ciccarelli; Marco Oliveti; Wolfgang A Linke; Ilona Cuijpers; Stephane Heymans; Nazha Hamdani; Martine de Boer; Dirk Duncker; Diederik Kuster; Jolanda van der Velden; Christophe Beauloye; Luc Bertrand; Manuel Mayr; Mauro Giacca; Florian Leuschner; Johannes Backs; Thomas Thum; Working Group on Myocardial Function of the European Society of Cardiology.

doi:10.1093/cvr/cvaa110

Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function

Serena Zacchigna^* (Corresponding Author), Alessia Paldino, Inês Falcão-Pires, Evangelos P Daskalopoulos, Matteo Dal Ferro, Simone Vodret, Pierluigi Lesizza, Antonio Cannatà, Daniela Miranda-Silva, André P Lourenço, Bruno Pinamonti, Gianfranco Sinagra, Florian Weinberger, Thomas Eschenhagen, Lucie Carrier, Izhak Kehat, Carlo G Tocchetti, Michele Russo, Alessandra Ghigo, James CiminoEmilio Hirsch, Dana Dawson, Michele Ciccarelli, Marco Oliveti, Wolfgang A Linke, Ilona Cuijpers, Stephane Heymans, Nazha Hamdani, Martine de Boer, Dirk Duncker, Diederik Kuster, Jolanda van der Velden, Christophe Beauloye, Luc Bertrand, Manuel Mayr, Mauro Giacca, Florian Leuschner, Johannes Backs, Thomas Thum, Working Group on Myocardial Function of the European Society of Cardiology.

^*Corresponding author for this work

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Abstract

Echocardiography is a reliable and reproducible method to assess non-invasively cardiac function in clinical and experimental research. Significant progress in the development of echocardiographic equipment and transducers has led to the successful translation of this methodology from humans to rodents, allowing for the scoring of disease severity and progression, testing of new drugs, and monitoring cardiac function in genetically modified or pharmacologically treated animals. However, as yet, there is no standardization in the procedure to acquire echocardiographic measurements in small animals. This position paper focuses on the appropriate acquisition and analysis of echocardiographic parameters in adult mice and rats, and provides reference values, representative images and videos for the accurate and reproducible quantification of left ventricular function in healthy and pathological conditions.

Original language	English
Article number	cvaa110
Pages (from-to)	43–59
Number of pages	37
Journal	Cardiovascular Research
Volume	117
Issue number	1
Early online date	4 May 2020
DOIs	https://doi.org/10.1093/cvr/cvaa110
Publication status	Published - 1 Jan 2021

Bibliographical note

This work was supported by AIRC IG grant 2016 19032 to S.Z.; FEDER through Compete 2020 –Programa Operacional Competitividade E Internacionalização(POCI), the project DOCNET (norte-01-0145-feder-000003), supported by Norte Portugal regional operational programme (norte 2020), under the Portugal 2020 partnership agreement, through the European Regional Development Fund (ERDF), the project NETDIAMOND (POCI-01-0145-FEDER-016385), supported by European Structural And Investment Funds, Lisbon’s regional operational program 2020 to I.P.F.; grants from FSR-FNRS, FRC (Cliniques Universitaires Saint-Luc) and from Action de Recherche Concertée (UCLouvain) to C.B., E.P.D. and L.B; the ERA-Net-CVD project MacroERA,01KL1706, FP7-Homage N° 305507, and IMI2-CARDIATEAM (N° 821508)to S.H.,the DZHK (German Centre for Cardiovascular Research) and the German Ministry of Research and Education (BMBF)to F.W., T.E. and L.C., the Netherlands Cardiovascular Research Initiative, an initiative with support of the Dutch Heart Foundation, CVON2016-Early HFPEF, 2015-10, CVON She-PREDICTS, grant 2017-21, CVON Arena-PRIME, 2017-18, Flemish Research FoundationFWO G091018N and FWO G0B5930N to S.H.; Federico II University/Ricerca di Ateneo grant to C.G..T.; the European Research Area Networks on Cardiovascular Diseases (ERA-CVD) [LYMIT-DIS 2016, MacroERA], Fonds Wetenschappelijk Onderzoek [1160718N] to I.C; the Deutsche Forschungsgemeinschaft (DFG TH903/20-1, KFO311), the Transregio-SFB INST 95/15641 and the EU Horizon 2020 project Cardioregenix (GA 825670)to T.T

Keywords

myochardium
ventricular function
echocardiography
rodentia
european society of cardiology
Diastolic function
Animal models
Echocardiography
Standardization
Systolic function

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This is a pre-copyedited, author-produced version of an article accepted for publication in Cardiovascular Research following peer review. The version of record will be available online at: https://academic.oup.com/cardiovascres/advance-article/doi/10.1093/cvr/cvaa110/5828935 This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
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Zacchigna, S., Paldino, A., Falcão-Pires, I., Daskalopoulos, E. P., Dal Ferro, M., Vodret, S., Lesizza, P., Cannatà, A., Miranda-Silva, D., Lourenço, A. P., Pinamonti, B., Sinagra, G., Weinberger, F., Eschenhagen, T., Carrier, L., Kehat, I., Tocchetti, C. G., Russo, M., Ghigo, A., ... Working Group on Myocardial Function of the European Society of Cardiology. (2021). Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function. Cardiovascular Research, 117(1), 43–59. Article cvaa110. https://doi.org/10.1093/cvr/cvaa110

Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function. / Zacchigna, Serena (Corresponding Author); Paldino, Alessia; Falcão-Pires, Inês et al.
In: Cardiovascular Research, Vol. 117, No. 1, cvaa110, 01.01.2021, p. 43–59.

Research output: Contribution to journal › Article › peer-review

Zacchigna, S, Paldino, A, Falcão-Pires, I, Daskalopoulos, EP, Dal Ferro, M, Vodret, S, Lesizza, P, Cannatà, A, Miranda-Silva, D, Lourenço, AP, Pinamonti, B, Sinagra, G, Weinberger, F, Eschenhagen, T, Carrier, L, Kehat, I, Tocchetti, CG, Russo, M, Ghigo, A, Cimino, J, Hirsch, E, Dawson, D, Ciccarelli, M, Oliveti, M, Linke, WA, Cuijpers, I, Heymans, S, Hamdani, N, de Boer, M, Duncker, D, Kuster, D, van der Velden, J, Beauloye, C, Bertrand, L, Mayr, M, Giacca, M, Leuschner, F, Backs, J, Thum, T & Working Group on Myocardial Function of the European Society of Cardiology. 2021, 'Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function', Cardiovascular Research, vol. 117, no. 1, cvaa110, pp. 43–59. https://doi.org/10.1093/cvr/cvaa110

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abstract = "Echocardiography is a reliable and reproducible method to assess non-invasively cardiac function in clinical and experimental research. Significant progress in the development of echocardiographic equipment and transducers has led to the successful translation of this methodology from humans to rodents, allowing for the scoring of disease severity and progression, testing of new drugs, and monitoring cardiac function in genetically modified or pharmacologically treated animals. However, as yet, there is no standardization in the procedure to acquire echocardiographic measurements in small animals. This position paper focuses on the appropriate acquisition and analysis of echocardiographic parameters in adult mice and rats, and provides reference values, representative images and videos for the accurate and reproducible quantification of left ventricular function in healthy and pathological conditions.",

keywords = "myochardium, ventricular function, echocardiography, rodentia, european society of cardiology, Diastolic function, Animal models, Echocardiography, Standardization, Systolic function",

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AU - Lesizza, Pierluigi

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AU - Ciccarelli, Michele

AU - Oliveti, Marco

AU - Linke, Wolfgang A

AU - Cuijpers, Ilona

AU - Heymans, Stephane

AU - Hamdani, Nazha

AU - de Boer, Martine

AU - Duncker, Dirk

AU - Kuster, Diederik

AU - van der Velden, Jolanda

AU - Beauloye, Christophe

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AU - Mayr, Manuel

AU - Giacca, Mauro

AU - Leuschner, Florian

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Towards standardization of echocardiography for the evaluation of left ventricular function in adult rodents: a position paper of the ESC Working Group on Myocardial Function

Abstract

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Keywords

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