Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans

Paul R. J. Birch, Miles Armstrong, Jorunn Bos, Petra Boevink, Eleanor M. Gilroy, Rosalind M. Taylor, Stephan Wawra, Leighton Pritchard, Lucio Conti, Richard Ewan, Stephen C. Whisson, Pieter van West, Ari Sadanandom, Sophien Kamoun

Research output: Contribution to journalLiterature review

61 Citations (Scopus)

Abstract

Plant pathogens establish infection by secretion of effector proteins that may be delivered inside host cells to manipulate innate immunity. It is increasingly apparent that the ubiquitin proteasome system (UPS) contributes significantly to the regulation of plant defences and, as such, is a target for pathogen effectors. Bacterial effectors delivered by the type III and IV secretion systems have been shown to interact with components of the host UPS. Some of these effectors possess functional domains that are conserved in UPS enzymes, whilst others contain novel domains with ubiquitination activities. Relatively little is known about effector activities in eukaryotic microbial plant pathogens. Nevertheless, effectors from oomycetes that contain an RXLR motif for translocation to the inside of plant cells have been shown to suppress host defences. Annotation of the genome of one such oomycete, the potato late blight pathogen Phytophthora infestans, and protein-protein interaction assays to discover host proteins targeted by the RXLR effector AVR3a, have revealed that this eukaryotic plant pathogen also has the potential to manipulate host plant UPS functions.

Original languageEnglish
Pages (from-to)1133-1140
Number of pages8
JournalJournal of Experimental Botany
Volume60
Issue number4
Early online date9 Feb 2009
DOIs
Publication statusPublished - Mar 2009

Keywords

  • E3 ubiquitin ligase
  • cell-death
  • downy mildew
  • microbe interactions
  • bacterial virulence
  • defence responses
  • resistance genes
  • innate immunity
  • promote disease
  • proteins

Cite this

Birch, P. R. J., Armstrong, M., Bos, J., Boevink, P., Gilroy, E. M., Taylor, R. M., ... Kamoun, S. (2009). Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans. Journal of Experimental Botany, 60(4), 1133-1140. https://doi.org/10.1093/jxb/ern353

Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans. / Birch, Paul R. J.; Armstrong, Miles; Bos, Jorunn; Boevink, Petra; Gilroy, Eleanor M.; Taylor, Rosalind M.; Wawra, Stephan; Pritchard, Leighton; Conti, Lucio; Ewan, Richard; Whisson, Stephen C.; van West, Pieter; Sadanandom, Ari; Kamoun, Sophien.

In: Journal of Experimental Botany, Vol. 60, No. 4, 03.2009, p. 1133-1140.

Research output: Contribution to journalLiterature review

Birch, PRJ, Armstrong, M, Bos, J, Boevink, P, Gilroy, EM, Taylor, RM, Wawra, S, Pritchard, L, Conti, L, Ewan, R, Whisson, SC, van West, P, Sadanandom, A & Kamoun, S 2009, 'Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans', Journal of Experimental Botany, vol. 60, no. 4, pp. 1133-1140. https://doi.org/10.1093/jxb/ern353
Birch, Paul R. J. ; Armstrong, Miles ; Bos, Jorunn ; Boevink, Petra ; Gilroy, Eleanor M. ; Taylor, Rosalind M. ; Wawra, Stephan ; Pritchard, Leighton ; Conti, Lucio ; Ewan, Richard ; Whisson, Stephen C. ; van West, Pieter ; Sadanandom, Ari ; Kamoun, Sophien. / Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans. In: Journal of Experimental Botany. 2009 ; Vol. 60, No. 4. pp. 1133-1140.
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abstract = "Plant pathogens establish infection by secretion of effector proteins that may be delivered inside host cells to manipulate innate immunity. It is increasingly apparent that the ubiquitin proteasome system (UPS) contributes significantly to the regulation of plant defences and, as such, is a target for pathogen effectors. Bacterial effectors delivered by the type III and IV secretion systems have been shown to interact with components of the host UPS. Some of these effectors possess functional domains that are conserved in UPS enzymes, whilst others contain novel domains with ubiquitination activities. Relatively little is known about effector activities in eukaryotic microbial plant pathogens. Nevertheless, effectors from oomycetes that contain an RXLR motif for translocation to the inside of plant cells have been shown to suppress host defences. Annotation of the genome of one such oomycete, the potato late blight pathogen Phytophthora infestans, and protein-protein interaction assays to discover host proteins targeted by the RXLR effector AVR3a, have revealed that this eukaryotic plant pathogen also has the potential to manipulate host plant UPS functions.",
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