Toxicity of mono, di and tri chlorophenols to lux marked terrestrial bacteria, Burkholderia species RASC C2 and Pseudomonas fluorescens

E. M. Boyd, Kenneth Stuart Killham, Andrew Alexander Meharg

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Burkholderia species RASC and Pseudomonas fluorescens were marked with lux genes, encoding for bioluminescence and used to assess the toxicity of mono-, di- and tri-chlorophenols by determining the decline in bioluminescence following exposure to the compounds in aqueous solution. Toxicity was expressed as a 50% effective concentration value (EC50), equating to the concentration of compound which caused a 50% decline in bioluminescence. Comparing the toxicity values of the compounds showed that, in general, increasing the degree of chlorination, increased toxicity. By carrying out forward multiple linear regressions with log(10) EC50 values and physio-chemical descriptors, it was shown that molecular parameters describing the hydrogen bonding nature of a chlorophenol provided a better fit than regressions between toxicity data and log(10) K-ow alone. Utilising these descriptor variables in equations, it was shown that the toxicity of chlorophenols to the lux marked bacteria could be predicted from the compounds physio-chemical characteristics. By correlating lux marked RASC c2 and P. fluorescens EC50 values with toxicity values using Pimephales promelas (fathead minnow), Tetrahymena pyriformis (ciliate) and marine bacterium Vibrio fischeri, it was apparent that lux marked RASC c2 correlated well with the freshwater aquatic species (P. promelas and T. pyriformis). This implied that for predictions of toxicity of organic xenobiotic compounds to higher organisms, lux marked RASC c2 could be utilised as a rapid surrogate. (C) 2001 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)157-166
Number of pages9
JournalChemosphere
Volume43
DOIs
Publication statusPublished - 2001

Keywords

  • Burkholderia
  • chlorophenol
  • lux
  • Pseudomonas fluorescens
  • toxicity
  • QUANTITATIVE STRUCTURE
  • BIOLUMINESCENT BACTERIA
  • CHEMICAL DESCRIPTORS
  • NONPOLAR NARCOTICS
  • VIBRIO-FISCHERI
  • SOIL
  • SEDIMENT
  • SYSTEMS
  • PHENOLS
  • ACID

Cite this

Toxicity of mono, di and tri chlorophenols to lux marked terrestrial bacteria, Burkholderia species RASC C2 and Pseudomonas fluorescens. / Boyd, E. M.; Killham, Kenneth Stuart; Meharg, Andrew Alexander.

In: Chemosphere, Vol. 43, 2001, p. 157-166.

Research output: Contribution to journalArticle

Boyd, E. M. ; Killham, Kenneth Stuart ; Meharg, Andrew Alexander. / Toxicity of mono, di and tri chlorophenols to lux marked terrestrial bacteria, Burkholderia species RASC C2 and Pseudomonas fluorescens. In: Chemosphere. 2001 ; Vol. 43. pp. 157-166.
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AB - Burkholderia species RASC and Pseudomonas fluorescens were marked with lux genes, encoding for bioluminescence and used to assess the toxicity of mono-, di- and tri-chlorophenols by determining the decline in bioluminescence following exposure to the compounds in aqueous solution. Toxicity was expressed as a 50% effective concentration value (EC50), equating to the concentration of compound which caused a 50% decline in bioluminescence. Comparing the toxicity values of the compounds showed that, in general, increasing the degree of chlorination, increased toxicity. By carrying out forward multiple linear regressions with log(10) EC50 values and physio-chemical descriptors, it was shown that molecular parameters describing the hydrogen bonding nature of a chlorophenol provided a better fit than regressions between toxicity data and log(10) K-ow alone. Utilising these descriptor variables in equations, it was shown that the toxicity of chlorophenols to the lux marked bacteria could be predicted from the compounds physio-chemical characteristics. By correlating lux marked RASC c2 and P. fluorescens EC50 values with toxicity values using Pimephales promelas (fathead minnow), Tetrahymena pyriformis (ciliate) and marine bacterium Vibrio fischeri, it was apparent that lux marked RASC c2 correlated well with the freshwater aquatic species (P. promelas and T. pyriformis). This implied that for predictions of toxicity of organic xenobiotic compounds to higher organisms, lux marked RASC c2 could be utilised as a rapid surrogate. (C) 2001 Elsevier Science Ltd. All rights reserved.

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