Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish

J J Campos-Perez, A E Ellis, C J Secombes

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O-2(-) donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalDiseases of Aquatic Organisms
Volume43
Publication statusPublished - 2000

Keywords

  • SNAP
  • SIN-1
  • nitric oxide
  • peroxynitrite
  • Renibacterium salmoninarum
  • Aeromonas salmonicida
  • Yersinia ruckeri
  • MACROPHAGE ACTIVATING FACTOR(S)
  • CATFISH ICTALURUS-PUNCTATUS
  • MYCOBACTERIUM-TUBERCULOSIS
  • RENIBACTERIUM-SALMONINARUM
  • MURINE MACROPHAGES
  • ESCHERICHIA-COLI
  • RAINBOW-TROUT
  • AEROMONAS-SALMONICIDA
  • CANDIDACIDAL ACTIVITY
  • ONCORHYNCHUS-MYKISS

Cite this

Campos-Perez, J. J., Ellis, A. E., & Secombes, C. J. (2000). Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish. Diseases of Aquatic Organisms, 43, 109-115.

Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish. / Campos-Perez, J J ; Ellis, A E ; Secombes, C J .

In: Diseases of Aquatic Organisms, Vol. 43, 2000, p. 109-115.

Research output: Contribution to journalArticle

Campos-Perez, JJ, Ellis, AE & Secombes, CJ 2000, 'Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish', Diseases of Aquatic Organisms, vol. 43, pp. 109-115.
Campos-Perez, J J ; Ellis, A E ; Secombes, C J . / Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish. In: Diseases of Aquatic Organisms. 2000 ; Vol. 43. pp. 109-115.
@article{2beec6b2ecb441a393f6f21af04f1f72,
title = "Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish",
abstract = "The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O-2(-) donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed.",
keywords = "SNAP, SIN-1, nitric oxide, peroxynitrite, Renibacterium salmoninarum, Aeromonas salmonicida, Yersinia ruckeri, MACROPHAGE ACTIVATING FACTOR(S), CATFISH ICTALURUS-PUNCTATUS, MYCOBACTERIUM-TUBERCULOSIS, RENIBACTERIUM-SALMONINARUM, MURINE MACROPHAGES, ESCHERICHIA-COLI, RAINBOW-TROUT, AEROMONAS-SALMONICIDA, CANDIDACIDAL ACTIVITY, ONCORHYNCHUS-MYKISS",
author = "Campos-Perez, {J J} and Ellis, {A E} and Secombes, {C J}",
year = "2000",
language = "English",
volume = "43",
pages = "109--115",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",

}

TY - JOUR

T1 - Toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish

AU - Campos-Perez, J J

AU - Ellis, A E

AU - Secombes, C J

PY - 2000

Y1 - 2000

N2 - The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O-2(-) donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed.

AB - The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O-2(-) donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed.

KW - SNAP

KW - SIN-1

KW - nitric oxide

KW - peroxynitrite

KW - Renibacterium salmoninarum

KW - Aeromonas salmonicida

KW - Yersinia ruckeri

KW - MACROPHAGE ACTIVATING FACTOR(S)

KW - CATFISH ICTALURUS-PUNCTATUS

KW - MYCOBACTERIUM-TUBERCULOSIS

KW - RENIBACTERIUM-SALMONINARUM

KW - MURINE MACROPHAGES

KW - ESCHERICHIA-COLI

KW - RAINBOW-TROUT

KW - AEROMONAS-SALMONICIDA

KW - CANDIDACIDAL ACTIVITY

KW - ONCORHYNCHUS-MYKISS

M3 - Article

VL - 43

SP - 109

EP - 115

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

ER -