Abstract
This study determined that the bacterial luciferase fusion gene (luxAB) was not a suitable in vivo gene reporter in the model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans. LuxAB expressing S. cerevisiae strains displayed distinctive rapid decays in luminescence upon addition of the bacterial luciferase substrate, n-decyl aldehyde, suggesting a toxic response. Growth studies and toxicity bioassays have subsequently confirmed, that the aldehyde substrate was toxic to both organisms at concentrations well tolerated by Escherichia coh. As the addition of aldehyde is an integral part of the bacterial luciferase activity assay, our results do not support the use of lux reporter genes for in vivo analyses in these model eukaryotic organisms. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 140-142 |
| Number of pages | 2 |
| Journal | FEBS Letters |
| Volume | 506 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 5 Oct 2001 |
Keywords
- biosensor
- luciferase
- bioluminescence
- toxicity
- vibrio-harveyi
- Escherichia-Coli
- fused gene
- expression
- fusion
- yeast
- eukaryotes
- LUXA
- DNA
Cite this
Toxicity of the bacterial luciferase substrate, n-decyl aldehyde, to Saccharomyces cerevisiae and Caenorhabditis elegans. / Hollis, R. P.; Lagido, Cristina; Pettitt, Jonathan; Porter, Andrew Justin Radcliffe; Killham, Kenneth Stuart; Paton, Graeme Iain; Glover, Lesley Anne.
In: FEBS Letters, Vol. 506, No. 2, 05.10.2001, p. 140-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxicity of the bacterial luciferase substrate, n-decyl aldehyde, to Saccharomyces cerevisiae and Caenorhabditis elegans
AU - Hollis, R. P.
AU - Lagido, Cristina
AU - Pettitt, Jonathan
AU - Porter, Andrew Justin Radcliffe
AU - Killham, Kenneth Stuart
AU - Paton, Graeme Iain
AU - Glover, Lesley Anne
N1 - Holis and Lagido are joint first authors
PY - 2001/10/5
Y1 - 2001/10/5
N2 - This study determined that the bacterial luciferase fusion gene (luxAB) was not a suitable in vivo gene reporter in the model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans. LuxAB expressing S. cerevisiae strains displayed distinctive rapid decays in luminescence upon addition of the bacterial luciferase substrate, n-decyl aldehyde, suggesting a toxic response. Growth studies and toxicity bioassays have subsequently confirmed, that the aldehyde substrate was toxic to both organisms at concentrations well tolerated by Escherichia coh. As the addition of aldehyde is an integral part of the bacterial luciferase activity assay, our results do not support the use of lux reporter genes for in vivo analyses in these model eukaryotic organisms. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
AB - This study determined that the bacterial luciferase fusion gene (luxAB) was not a suitable in vivo gene reporter in the model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans. LuxAB expressing S. cerevisiae strains displayed distinctive rapid decays in luminescence upon addition of the bacterial luciferase substrate, n-decyl aldehyde, suggesting a toxic response. Growth studies and toxicity bioassays have subsequently confirmed, that the aldehyde substrate was toxic to both organisms at concentrations well tolerated by Escherichia coh. As the addition of aldehyde is an integral part of the bacterial luciferase activity assay, our results do not support the use of lux reporter genes for in vivo analyses in these model eukaryotic organisms. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
KW - biosensor
KW - luciferase
KW - bioluminescence
KW - toxicity
KW - vibrio-harveyi
KW - Escherichia-Coli
KW - fused gene
KW - expression
KW - fusion
KW - yeast
KW - eukaryotes
KW - LUXA
KW - DNA
U2 - 10.1016/S0014-5793(01)02905-2
DO - 10.1016/S0014-5793(01)02905-2
M3 - Article
VL - 506
SP - 140
EP - 142
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -