TracMouse

A computer aided movement analysis script for the mouse inverted horizontal grid test

W Niewiadomski, E Palasz, M Skupinska, M Zylinski, M Steczkowska, A Gasiorowska, G Niewiadomska, G Riedel

Research output: Contribution to journalArticle

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Abstract

In rodents, detection and quantification of motor impairments is difficult. The traction test (inverted grid with mice clinging to the underside) currently has no objective rating system. We here developed and validated the semi-automatic MATLAB script TracMouse for unbiased detection of video-recorded movement patterns. High precision videos were analyzed by: (i) principal identification of anatomical paw details frame-by-frame by an experimentally blinded rater; (ii) automatic retrieval of proxies by TracMouse for individual paws. The basic states of Hold and Step were discriminated as duration and frequency, and these principle parameters were converted into static and dynamic endpoints and their discriminating power assessed in a dopaminergic lesion model. Relative to hind paws, forepaws performed ~4 times more steps, they were ~20% longer, and Hold duration was ~5 times shorter in normal C57Bl/6 mice. Thus, forepaw steps were classified as exploratory, hind paw movement as locomotive. Multiple novel features pertaining to paw sequence, step lengths and exploratory touches were accessible through TracMouse and revealed subtle Parkinsonian phenotypes. Novel proxies using TracMouse revealed previously unidentified features of movement and may aid the understanding of (i) brain circuits related to motor planning and execution, and (ii) phenotype detection in experimental models of movement disorders.

Original languageEnglish
Article number39331
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 16 Dec 2016

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Proxy
Phenotype
Movement Disorders
Touch
Traction
Rodentia
Theoretical Models
Brain
Power (Psychology)

Keywords

  • neurological disorders
  • neuroscience

Cite this

Niewiadomski, W., Palasz, E., Skupinska, M., Zylinski, M., Steczkowska, M., Gasiorowska, A., ... Riedel, G. (2016). TracMouse: A computer aided movement analysis script for the mouse inverted horizontal grid test. Scientific Reports, 6, [39331]. https://doi.org/10.1038/srep39331

TracMouse : A computer aided movement analysis script for the mouse inverted horizontal grid test. / Niewiadomski, W; Palasz, E; Skupinska, M; Zylinski, M; Steczkowska, M; Gasiorowska, A; Niewiadomska, G; Riedel, G.

In: Scientific Reports, Vol. 6, 39331, 16.12.2016.

Research output: Contribution to journalArticle

Niewiadomski, W, Palasz, E, Skupinska, M, Zylinski, M, Steczkowska, M, Gasiorowska, A, Niewiadomska, G & Riedel, G 2016, 'TracMouse: A computer aided movement analysis script for the mouse inverted horizontal grid test', Scientific Reports, vol. 6, 39331. https://doi.org/10.1038/srep39331
Niewiadomski W, Palasz E, Skupinska M, Zylinski M, Steczkowska M, Gasiorowska A et al. TracMouse: A computer aided movement analysis script for the mouse inverted horizontal grid test. Scientific Reports. 2016 Dec 16;6. 39331. https://doi.org/10.1038/srep39331
Niewiadomski, W ; Palasz, E ; Skupinska, M ; Zylinski, M ; Steczkowska, M ; Gasiorowska, A ; Niewiadomska, G ; Riedel, G. / TracMouse : A computer aided movement analysis script for the mouse inverted horizontal grid test. In: Scientific Reports. 2016 ; Vol. 6.
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