Trained immunity or tolerance

opposing functional programs induced in human monocytes after engagement of various pattern recognition receptors

Daniela C Ifrim, Jessica Quintin, Leo A B Joosten, Cor Jacobs, Trees Jansen, Liesbeth Jacobs, Neil A R Gow, David L Williams, Jos W M van der Meer, Mihai G Netea

Research output: Contribution to journalArticle

78 Citations (Scopus)
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Abstract

Upon priming with Candida albicans or with the fungal cell wall component β-glucan, monocytes respond with an increased cytokine production upon restimulation, a phenomenon termed "trained immunity." In contrast, the prestimulation of monocytes with lipopolysaccharide has long been known to induce tolerance. Because the vast majority of commensal microorganisms belong to bacterial or viral phyla, we sought to systematically investigate the functional reprogramming of monocytes induced by the stimulation of pattern recognition receptors (PRRs) with various bacterial or viral ligands. Monocytes were functionally programmed for either enhanced (training) or decreased (tolerance) cytokine production, depending on the type and concentration of ligand they encountered. The functional reprogramming of monocytes was also associated with cell shape, granulocity, and cell surface marker modifications. The training effect required p38- and Jun N-terminal protein kinase (JNK)-mediated mitogen-activated protein kinase (MAPK) signaling, with specific signaling patterns directing the functional fate of the cell. The long-term effects on the function of monocytes were mediated by epigenetic events, with both histone methylation and acetylation inhibitors blocking the training effects. In conclusion, our experiments identify the ability of monocytes to acquire adaptive characteristics after prior activation with a wide variety of ligands. Trained immunity and tolerance are two distinct and opposing functional programs induced by the specific microbial ligands engaging the monocytes.

Original languageEnglish
Pages (from-to)534-545
Number of pages12
JournalClinical and Vaccine Immunology
Volume21
Issue number4
Early online date12 Feb 2014
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Pattern Recognition Receptors
Monocytes
Immunity
Ligands
Cytokines
Acetylation
Methylation
Glucans
Candida
Mitogen-Activated Protein Kinases
Microorganisms
Histones
Protein Kinases
Lipopolysaccharides
Chemical activation
Cells
Cell Shape
Cellular Structures
Candida albicans
Epigenomics

Keywords

  • Antigens, Bacterial
  • Antigens, Viral
  • Bacteria
  • Candida albicans
  • Cytokines
  • Humans
  • Immune Tolerance
  • Monocytes
  • Receptors, Pattern Recognition
  • Viruses

Cite this

Trained immunity or tolerance : opposing functional programs induced in human monocytes after engagement of various pattern recognition receptors. / Ifrim, Daniela C; Quintin, Jessica; Joosten, Leo A B; Jacobs, Cor; Jansen, Trees; Jacobs, Liesbeth; Gow, Neil A R; Williams, David L; van der Meer, Jos W M; Netea, Mihai G.

In: Clinical and Vaccine Immunology, Vol. 21, No. 4, 04.2014, p. 534-545.

Research output: Contribution to journalArticle

Ifrim, Daniela C ; Quintin, Jessica ; Joosten, Leo A B ; Jacobs, Cor ; Jansen, Trees ; Jacobs, Liesbeth ; Gow, Neil A R ; Williams, David L ; van der Meer, Jos W M ; Netea, Mihai G. / Trained immunity or tolerance : opposing functional programs induced in human monocytes after engagement of various pattern recognition receptors. In: Clinical and Vaccine Immunology. 2014 ; Vol. 21, No. 4. pp. 534-545.
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title = "Trained immunity or tolerance: opposing functional programs induced in human monocytes after engagement of various pattern recognition receptors",
abstract = "Upon priming with Candida albicans or with the fungal cell wall component β-glucan, monocytes respond with an increased cytokine production upon restimulation, a phenomenon termed {"}trained immunity.{"} In contrast, the prestimulation of monocytes with lipopolysaccharide has long been known to induce tolerance. Because the vast majority of commensal microorganisms belong to bacterial or viral phyla, we sought to systematically investigate the functional reprogramming of monocytes induced by the stimulation of pattern recognition receptors (PRRs) with various bacterial or viral ligands. Monocytes were functionally programmed for either enhanced (training) or decreased (tolerance) cytokine production, depending on the type and concentration of ligand they encountered. The functional reprogramming of monocytes was also associated with cell shape, granulocity, and cell surface marker modifications. The training effect required p38- and Jun N-terminal protein kinase (JNK)-mediated mitogen-activated protein kinase (MAPK) signaling, with specific signaling patterns directing the functional fate of the cell. The long-term effects on the function of monocytes were mediated by epigenetic events, with both histone methylation and acetylation inhibitors blocking the training effects. In conclusion, our experiments identify the ability of monocytes to acquire adaptive characteristics after prior activation with a wide variety of ligands. Trained immunity and tolerance are two distinct and opposing functional programs induced by the specific microbial ligands engaging the monocytes.",
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note = "Article Accepted Date: 29 January 2014. ACKNOWLEDGMENTS D.C.I. received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement HEALTH-2010-260338 (“Fungi in the setting of inflammation, allergy and autoimmune diseases: translating basic science into clinical practices” [ALLFUN]) (awarded to M.G.N.). M.G.N. and J.Q. were supported by a Vici grant of the Netherlands Organization of Scientific Research (awarded to M.G.N.). This work was supported, in part, by National Institutes of Health grant GM53522 to D.L.W. N.A.R.G. was supported by the Wellcome Trust.",
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AU - Ifrim, Daniela C

AU - Quintin, Jessica

AU - Joosten, Leo A B

AU - Jacobs, Cor

AU - Jansen, Trees

AU - Jacobs, Liesbeth

AU - Gow, Neil A R

AU - Williams, David L

AU - van der Meer, Jos W M

AU - Netea, Mihai G

N1 - Article Accepted Date: 29 January 2014. ACKNOWLEDGMENTS D.C.I. received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement HEALTH-2010-260338 (“Fungi in the setting of inflammation, allergy and autoimmune diseases: translating basic science into clinical practices” [ALLFUN]) (awarded to M.G.N.). M.G.N. and J.Q. were supported by a Vici grant of the Netherlands Organization of Scientific Research (awarded to M.G.N.). This work was supported, in part, by National Institutes of Health grant GM53522 to D.L.W. N.A.R.G. was supported by the Wellcome Trust.

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N2 - Upon priming with Candida albicans or with the fungal cell wall component β-glucan, monocytes respond with an increased cytokine production upon restimulation, a phenomenon termed "trained immunity." In contrast, the prestimulation of monocytes with lipopolysaccharide has long been known to induce tolerance. Because the vast majority of commensal microorganisms belong to bacterial or viral phyla, we sought to systematically investigate the functional reprogramming of monocytes induced by the stimulation of pattern recognition receptors (PRRs) with various bacterial or viral ligands. Monocytes were functionally programmed for either enhanced (training) or decreased (tolerance) cytokine production, depending on the type and concentration of ligand they encountered. The functional reprogramming of monocytes was also associated with cell shape, granulocity, and cell surface marker modifications. The training effect required p38- and Jun N-terminal protein kinase (JNK)-mediated mitogen-activated protein kinase (MAPK) signaling, with specific signaling patterns directing the functional fate of the cell. The long-term effects on the function of monocytes were mediated by epigenetic events, with both histone methylation and acetylation inhibitors blocking the training effects. In conclusion, our experiments identify the ability of monocytes to acquire adaptive characteristics after prior activation with a wide variety of ligands. Trained immunity and tolerance are two distinct and opposing functional programs induced by the specific microbial ligands engaging the monocytes.

AB - Upon priming with Candida albicans or with the fungal cell wall component β-glucan, monocytes respond with an increased cytokine production upon restimulation, a phenomenon termed "trained immunity." In contrast, the prestimulation of monocytes with lipopolysaccharide has long been known to induce tolerance. Because the vast majority of commensal microorganisms belong to bacterial or viral phyla, we sought to systematically investigate the functional reprogramming of monocytes induced by the stimulation of pattern recognition receptors (PRRs) with various bacterial or viral ligands. Monocytes were functionally programmed for either enhanced (training) or decreased (tolerance) cytokine production, depending on the type and concentration of ligand they encountered. The functional reprogramming of monocytes was also associated with cell shape, granulocity, and cell surface marker modifications. The training effect required p38- and Jun N-terminal protein kinase (JNK)-mediated mitogen-activated protein kinase (MAPK) signaling, with specific signaling patterns directing the functional fate of the cell. The long-term effects on the function of monocytes were mediated by epigenetic events, with both histone methylation and acetylation inhibitors blocking the training effects. In conclusion, our experiments identify the ability of monocytes to acquire adaptive characteristics after prior activation with a wide variety of ligands. Trained immunity and tolerance are two distinct and opposing functional programs induced by the specific microbial ligands engaging the monocytes.

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KW - Antigens, Viral

KW - Bacteria

KW - Candida albicans

KW - Cytokines

KW - Humans

KW - Immune Tolerance

KW - Monocytes

KW - Receptors, Pattern Recognition

KW - Viruses

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EP - 545

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 4

ER -