TY - JOUR
T1 - Trans-Dichloridopalladium(II) and platinum(II) complexes with 2-(hydroxymethyl)pyridine and 2-(2-hydroxyethyl)pyridine
T2 - Synthesis, structural characterization, DNA binding and in vitro cytotoxicity studies
AU - Icsel, Ceyda
AU - Yilmaz, Veysel T.
AU - Ari, Ferda
AU - Ulukaya, Engin
AU - Harrison, William T. A.
N1 - Acknowledgments
This work is a part of a research project 111T099. We thank TUBITAK for the financial support given to the project.
PY - 2013/2
Y1 - 2013/2
N2 - Four trans-palladium(II)- and trans-platinum(II)-chlorido complexes, trans-[PdCl2(2-hmpy)2] (1), trans-[PtCl 2(2-hmpy)2] (2), trans-[PdCl2(2-hepy) 2] (3) and trans-[PtCl2(2-hepy)2] (4) (2-hmpy = 2-(hydroxymethyl)pyridine and 2-hepy = 2-(2-hydroxyethyl)pyridine), have been synthesized and characterized by elemental analysis, IR, NMR, and X-ray diffraction. The binding properties of these complexes with fish sperm DNA (FS-DNA) were investigated by UV titration, viscosity, thermal denaturation and electrophoresis measurements. The complexes can bind to FS-DNA and complex 4 exhibits the highest binding constant. Gel electrophoresis assay demonstrates that all the complexes can cleave the pCMV-βgal plasmid DNA to a different degree. The cytotoxic activities of the complexes were tested against four different cancer cell lines. In general, the platinum(II) complexes are more effective than the isostructural palladium(II) complexes. Complex 4 shows high anticancer activity, compared to transplatin, cisplatin, carboplatin and oxaliplatin.
AB - Four trans-palladium(II)- and trans-platinum(II)-chlorido complexes, trans-[PdCl2(2-hmpy)2] (1), trans-[PtCl 2(2-hmpy)2] (2), trans-[PdCl2(2-hepy) 2] (3) and trans-[PtCl2(2-hepy)2] (4) (2-hmpy = 2-(hydroxymethyl)pyridine and 2-hepy = 2-(2-hydroxyethyl)pyridine), have been synthesized and characterized by elemental analysis, IR, NMR, and X-ray diffraction. The binding properties of these complexes with fish sperm DNA (FS-DNA) were investigated by UV titration, viscosity, thermal denaturation and electrophoresis measurements. The complexes can bind to FS-DNA and complex 4 exhibits the highest binding constant. Gel electrophoresis assay demonstrates that all the complexes can cleave the pCMV-βgal plasmid DNA to a different degree. The cytotoxic activities of the complexes were tested against four different cancer cell lines. In general, the platinum(II) complexes are more effective than the isostructural palladium(II) complexes. Complex 4 shows high anticancer activity, compared to transplatin, cisplatin, carboplatin and oxaliplatin.
KW - 2-(2-Hydroxyethyl) pyridine
KW - 2-(Hydroxymethyl)pyridine
KW - Cytotoxic activity
KW - DNA binding
KW - Transplatinum complexes
UR - http://www.scopus.com/inward/record.url?scp=84872160407&partnerID=8YFLogxK
U2 - 10.1016/j.ejmech.2012.12.002
DO - 10.1016/j.ejmech.2012.12.002
M3 - Article
C2 - 23314052
AN - SCOPUS:84872160407
VL - 60
SP - 386
EP - 394
JO - European Journal of Medicinal Chemistry
JF - European Journal of Medicinal Chemistry
SN - 0223-5234
ER -
