Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1

A. M. Murad, C. d'Enfert, C. Gaillardin, Helene Tournu, F. Tekaia, D. Talibi, D. Marechal, V. Marchais, J. Cottin

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Abstract

The pathogenic fungus, Candida albicans contains homologues of the transcriptional repressors ScTup1, ScMig1 and ScNrg1 found in budding yeast. In Saccharomyces cerevisiae, ScMig1 targets the ScTup1/ScSsn6 complex to the promoters of glucose repressed genes to repress their transcription. ScNrg1 is thought to act in a similar manner at other promoters. We have examined the roles of their homologues in C. albicans by transcript profiling with an array containing 2002 genes, representing about one quarter of the predicted number of open reading frames (ORFs) in C. albicans. The data revealed that CaNrg1 and CaTup1 regulate a different set of C. albicans genes from CaMig1 and CaTup1. This is consistent with the idea that CaMig1 and CaNrg1 target the CaTup1 repressor to specific subsets of C. albicans genes. However, CaMig1 and CaNrg1 repress other C. albicans genes in a CaTup1-independent fashion. The targets of CaMig1 and CaNrg1 repression, and phenotypic analyses of nrg1/nrg1 and mig1/mig1 mutants, indicate that these factors play differential roles in the regulation of metabolism, cellular morphogenesis and stress responses. Hence, the data provide important information both about the modes of action of these transcriptional regulators and their cellular roles. The transcript profiling data are available at http://www.pasteur.fr/recherche/ unites/RIF/transcriptdata/.

Original languageEnglish
Pages (from-to)981-993
Number of pages12
JournalMolecular Microbiology
Volume42
Issue number4
DOIs
Publication statusPublished - Nov 2001

Keywords

  • saccharomyces-cerevisiae
  • gene-expression
  • catabolite repression
  • yeast genome
  • virulence
  • SSN6-TUP1
  • disruption
  • protein
  • TUP1
  • MIG1

Cite this

Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1. / Murad, A. M.; d'Enfert, C.; Gaillardin, C.; Tournu, Helene; Tekaia, F.; Talibi, D.; Marechal, D.; Marchais, V.; Cottin, J.

In: Molecular Microbiology, Vol. 42, No. 4, 11.2001, p. 981-993.

Research output: Contribution to journalArticle

Murad, AM, d'Enfert, C, Gaillardin, C, Tournu, H, Tekaia, F, Talibi, D, Marechal, D, Marchais, V & Cottin, J 2001, 'Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1' Molecular Microbiology, vol. 42, no. 4, pp. 981-993. https://doi.org/10.1046/j.1365-2958.2001.02713.x
Murad, A. M. ; d'Enfert, C. ; Gaillardin, C. ; Tournu, Helene ; Tekaia, F. ; Talibi, D. ; Marechal, D. ; Marchais, V. ; Cottin, J. / Transcript profiling in Candida albicans reveals new cellular functions for the transcriptional repressors CaTup1, CaMig1 and CaNrg1. In: Molecular Microbiology. 2001 ; Vol. 42, No. 4. pp. 981-993.
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AU - d'Enfert, C.

AU - Gaillardin, C.

AU - Tournu, Helene

AU - Tekaia, F.

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AU - Marechal, D.

AU - Marchais, V.

AU - Cottin, J.

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AB - The pathogenic fungus, Candida albicans contains homologues of the transcriptional repressors ScTup1, ScMig1 and ScNrg1 found in budding yeast. In Saccharomyces cerevisiae, ScMig1 targets the ScTup1/ScSsn6 complex to the promoters of glucose repressed genes to repress their transcription. ScNrg1 is thought to act in a similar manner at other promoters. We have examined the roles of their homologues in C. albicans by transcript profiling with an array containing 2002 genes, representing about one quarter of the predicted number of open reading frames (ORFs) in C. albicans. The data revealed that CaNrg1 and CaTup1 regulate a different set of C. albicans genes from CaMig1 and CaTup1. This is consistent with the idea that CaMig1 and CaNrg1 target the CaTup1 repressor to specific subsets of C. albicans genes. However, CaMig1 and CaNrg1 repress other C. albicans genes in a CaTup1-independent fashion. The targets of CaMig1 and CaNrg1 repression, and phenotypic analyses of nrg1/nrg1 and mig1/mig1 mutants, indicate that these factors play differential roles in the regulation of metabolism, cellular morphogenesis and stress responses. Hence, the data provide important information both about the modes of action of these transcriptional regulators and their cellular roles. The transcript profiling data are available at http://www.pasteur.fr/recherche/ unites/RIF/transcriptdata/.

KW - saccharomyces-cerevisiae

KW - gene-expression

KW - catabolite repression

KW - yeast genome

KW - virulence

KW - SSN6-TUP1

KW - disruption

KW - protein

KW - TUP1

KW - MIG1

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