Transcription factor cycling on the insulin promoter

John Barrow, Colin Hay, Laura Ann Ferguson, Hilary Docherty, Kevin Docherty

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Using MIN6 beta-cells and chromatin immunoprecipitation (ChIP) assays, the chronological sequence of binding of MafA, E47/beta 2 and PDX-1 to the insulin promoter in living P-cells were investigated. All four factors were shown to bind to the mouse insulin 2 promoter in a cyclical manner with a periodicity of approximately 10-15 min. The cyclical binding of MafA, E47 and beta 2 was largely unaffected by the glucose or insulin concentration in the media. However, the binding and cycling of PDX-1 was markedly abolished in low glucose (1 mM), and this was reversed in the presence of low concentrations of insulin. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)711-715
Number of pages5
JournalFEBS Letters
Volume580
Issue number2
Early online date28 Dec 2005
DOIs
Publication statusPublished - 23 Jan 2006

Keywords

  • Animals
  • Base Sequence
  • Cell Line
  • Glucose
  • Homeodomain Proteins
  • Humans
  • Insulin
  • Insulin-Secreting Cells
  • Maf Transcription Factors, Large
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Protein Binding
  • Sequence Alignment
  • TCF Transcription Factors
  • Trans-Activators

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