Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status

Aileen M Halliday, F Alex Lainson, Raja Yaga, Neil F Inglis, Stephen Bridgett, Mintu Nath, Dave P Knox

Research output: Contribution to journalArticle

Abstract

The aim of this study was to elucidate transcriptional changes in the parasitic nematode Teladorsagia circumcincta upon encountering either naïve or immune ovine hosts. Pools of 100 000 exsheathed 3rd- stage T. circumcincta larvae were exposed in vitro to either an immune or naïve ovine abomasal environment, RNA was extracted from the larvae and sequenced using the Roche 454 platform. Each sample produced approximately 82 000 reads that assembled to give approximately 5500 Isotigs (contigs). The two sequence datasets were clustered together to give a total of 6969 clusters of which 18 were differentially expressed (P<0·001) between the two groups. Clusters with a predominance of reads in larvae exposed to the immune abomasal environment encoded homologues of peptidyl-glycine alpha-amidating monooxygenase, heat shock-protein 16-2 and IDA-1, a tyrosine phosphatase-like receptor protein. Clusters with a predominance of reads in the naïve environment encoded homologues of cytochrome b, EGg Laying defective family member 21 and NADH dehydrogenase subunit 5. Gene ontology analyses indicated that larvae exposed to the immune environment showed an increase in expression of genes involved in 'carbon utilization', 'response to stimulus' and 'developmental process'. These data suggest that T. circumcincta modulates gene expression in response to the immune status of the host.

Original languageEnglish
Pages (from-to)387-405
Number of pages19
JournalParasitology
Volume139
Issue number3
Early online date5 Jan 2012
DOIs
Publication statusPublished - Mar 2012

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Teladorsagia circumcincta
Larva
larvae
Receptor-Like Protein Tyrosine Phosphatases
Sheep
animal parasitic nematodes
Gene Expression
NADH Dehydrogenase
sheep
gene expression
Gene Ontology
Cytochromes b
NADH dehydrogenase
Heat-Shock Proteins
heat shock proteins
glycine (amino acid)
cytochrome b
Ovum
tyrosine
Carbon

Keywords

  • Abomasum/immunology
  • Adaptive Immunity
  • Animals
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Helminth Proteins/genetics
  • Host-Parasite Interactions
  • Larva/genetics
  • Proteomics
  • RNA, Messenger/metabolism
  • Sequence Analysis, DNA
  • Sheep
  • Sheep Diseases/immunology
  • Sheep, Domestic
  • Tissue Extracts
  • Trichostrongyloidea/genetics
  • Trichostrongyloidiasis/immunology

Cite this

Halliday, A. M., Lainson, F. A., Yaga, R., Inglis, N. F., Bridgett, S., Nath, M., & Knox, D. P. (2012). Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status. Parasitology, 139(3), 387-405. https://doi.org/10.1017/S0031182011002010

Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status. / Halliday, Aileen M; Lainson, F Alex; Yaga, Raja; Inglis, Neil F; Bridgett, Stephen; Nath, Mintu; Knox, Dave P.

In: Parasitology, Vol. 139, No. 3, 03.2012, p. 387-405.

Research output: Contribution to journalArticle

Halliday, Aileen M ; Lainson, F Alex ; Yaga, Raja ; Inglis, Neil F ; Bridgett, Stephen ; Nath, Mintu ; Knox, Dave P. / Transcriptional changes in Teladorsagia circumcincta upon encountering host tissue of differing immune status. In: Parasitology. 2012 ; Vol. 139, No. 3. pp. 387-405.
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