Transcriptome response following administration of a live bacterial vaccine in Atlantic salmon (Salmo salar)

Samuel Allen Moore Martin, Susan Corral Blaney, Dominic Francis Joseph Houlihan, Christopher John Secombes

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA-) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was collected from fish 6. 12, 24 and 48 h following infection or at the same time points from fish injected with PBS as non-infected control. PCR-select cDNA subtraction libraries were constructed from pooled 24 and 48 h post infection RNA to identify up-regulated mRNAs. One thousand four hundred and eighty six cDNA clones were sequenced from enriched cDNA libraries, of which 71% had significant homologies to known functional proteins. Many of these clones have previously been uncharacterised in Atlantic salmon. A salmonid cDNA microarray was used to further analyse the gene expression profile as the library construction in itself does not answer the dynamics of the response. The greatest increase in expression identified in the array analysis was a liver antibacterial peptide, hepcidin that was increased 11-fold following the challenge. A panel of clones were chosen for semiquantitative reverse transcriptase PCR from all time points sampled. These results indicated there were both temporal differences and tissue differences in the transcriptional response to bacterial exposure, potentially of relevance to the establishment of protection. (c) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1900-1911
Number of pages11
JournalMolecular Immunology
Volume43
Issue number11
Early online date28 Nov 2005
DOIs
Publication statusPublished - Apr 2006

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Bacterial Vaccines
Salmo salar
Transcriptome
Clone Cells
Gene Library
Fishes
Aeromonas salmonicida
Head Kidney
RNA
Hepcidins
Liver
Infection
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Libraries
Immunity
Complementary DNA
Polymerase Chain Reaction
Messenger RNA
Peptides

Keywords

  • microarray
  • Salmo salar
  • bacterial response
  • aeromonas salmonicida
  • subtractive cloning
  • trout oncorhynchus
  • suppression subtractive hybridization
  • rainbow-trout
  • aeromonas-salmonicida
  • antimicrobial peptides
  • innate immunity
  • cc chemokines
  • japanese flounder
  • cdna microarray
  • genes

Cite this

Transcriptome response following administration of a live bacterial vaccine in Atlantic salmon (Salmo salar). / Martin, Samuel Allen Moore; Blaney, Susan Corral; Houlihan, Dominic Francis Joseph; Secombes, Christopher John.

In: Molecular Immunology, Vol. 43, No. 11, 04.2006, p. 1900-1911.

Research output: Contribution to journalArticle

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