Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic

D T Thwaites, G T McEwan, B H Hirst, N L Simmons

Research output: Contribution to journalArticle

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Abstract

Net transepithelial transport (and cellular accumulation) of the dipeptide glycylsarcosine (Gly-Sar), across the apical membrane of human intestinal Caco-2 epithelia, is driven by a proton gradient (Na(+)-free conditions) and displays saturation kinetics (Km 17.4 +/- 5.1 mM, Vmax of 92.8 +/- 15.6 nmol.cm-2.h-1). Net Gly-Sar transport is associated with the stimulation of an inward short-circuit current (Isc). This dipeptide-stimulated Isc is observed in both Na(+)-containing and Na(+)-free conditions, is stimulated by apical acidity, and displays saturation kinetics (in Na(+)-free media at apical pH 6.0, Km of 13.6 +/- 4.5 mM and a Vmax of 284.1 +/- 39.3 nmol.cm-2.h-1). The maximal capacities of Gly-Sar transport and Isc suggest a dipeptide/proton stoichiometry greater than unity (1:3).
Original languageEnglish
Pages (from-to)178-80
Number of pages3
JournalPflugers Archiv : European Journal of Physiology
Volume425
Issue number1-2
Publication statusPublished - Oct 1993

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Caco-2 Cells
Dipeptides
Short circuit currents
Monolayers
Protons
Kinetics
Acidity
Stoichiometry
Epithelium
Membranes
glycylsarcosine

Keywords

  • Biological Transport
  • Cell Line
  • Dipeptides
  • Electric Conductivity
  • Epithelium
  • Humans
  • Hydrogen-Ion Concentration
  • Intestines
  • Kinetics
  • Protons

Cite this

Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic. / Thwaites, D T; McEwan, G T; Hirst, B H; Simmons, N L.

In: Pflugers Archiv : European Journal of Physiology, Vol. 425, No. 1-2, 10.1993, p. 178-80.

Research output: Contribution to journalArticle

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T1 - Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic

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AU - McEwan, G T

AU - Hirst, B H

AU - Simmons, N L

PY - 1993/10

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N2 - Net transepithelial transport (and cellular accumulation) of the dipeptide glycylsarcosine (Gly-Sar), across the apical membrane of human intestinal Caco-2 epithelia, is driven by a proton gradient (Na(+)-free conditions) and displays saturation kinetics (Km 17.4 +/- 5.1 mM, Vmax of 92.8 +/- 15.6 nmol.cm-2.h-1). Net Gly-Sar transport is associated with the stimulation of an inward short-circuit current (Isc). This dipeptide-stimulated Isc is observed in both Na(+)-containing and Na(+)-free conditions, is stimulated by apical acidity, and displays saturation kinetics (in Na(+)-free media at apical pH 6.0, Km of 13.6 +/- 4.5 mM and a Vmax of 284.1 +/- 39.3 nmol.cm-2.h-1). The maximal capacities of Gly-Sar transport and Isc suggest a dipeptide/proton stoichiometry greater than unity (1:3).

AB - Net transepithelial transport (and cellular accumulation) of the dipeptide glycylsarcosine (Gly-Sar), across the apical membrane of human intestinal Caco-2 epithelia, is driven by a proton gradient (Na(+)-free conditions) and displays saturation kinetics (Km 17.4 +/- 5.1 mM, Vmax of 92.8 +/- 15.6 nmol.cm-2.h-1). Net Gly-Sar transport is associated with the stimulation of an inward short-circuit current (Isc). This dipeptide-stimulated Isc is observed in both Na(+)-containing and Na(+)-free conditions, is stimulated by apical acidity, and displays saturation kinetics (in Na(+)-free media at apical pH 6.0, Km of 13.6 +/- 4.5 mM and a Vmax of 284.1 +/- 39.3 nmol.cm-2.h-1). The maximal capacities of Gly-Sar transport and Isc suggest a dipeptide/proton stoichiometry greater than unity (1:3).

KW - Biological Transport

KW - Cell Line

KW - Dipeptides

KW - Electric Conductivity

KW - Epithelium

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Intestines

KW - Kinetics

KW - Protons

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VL - 425

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JO - Pflugers Archiv : European Journal of Physiology

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