Abstract
New shuttle vectors based on a Prevotella ruminicola 9.5 kb cryptic plasmid (pRRI7) inserted within the Escherichia coli vector pKC71, carrying the Cc(r)/Em(r) Bacteroides marker, were constructed. These constructs (pKBR23-1 and pKBR23-2) were transferred into Bacteroides distasonis, Bacteroides thetaiotaomicron, Bacteroides uniforms and into P. ruminicola NCFB 2202 either by conjugal mobilization or by electroporation. Another pRRI7 derivative based on pKC72, PKBR23-3, was smaller (13.1 kb) and non-mobilizable. By electroporation, it was transferred to Bact. distasonis and P. ruminicola. Being derived from pRRI7 which is compatible with the shuttle plasmid pRRI207, the host/vector combination involving P. ruminicola NCFB 2202 and pKBR23-3 offers new possibilities for genetic investigations in rumen anaerobic bacteria after further introduction of a second readily selectable marker within pRRI207 or pKBR23-3.
Original language | English |
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Pages (from-to) | 542-548 |
Number of pages | 7 |
Journal | Journal of Applied Bacteriology |
Volume | 74 |
Issue number | 5 |
Publication status | Published - May 1993 |
Keywords
- AGROBACTERIUM-TUMEFACIENS
- RUMINAL STRAINS
- RESISTANCE GENE
- XYLANASE GENE
- TI-PLASMIDS
- RUMINICOLA
- CLONING
- EXPRESSION
- UNIFORMIS
- FRAGILIS