Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva

D K Mercer, K P Scott, C M Melville, L A Glover, Harry James Flint

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Transformation of Streptococcus gordonii DL1 by free DNA was studied in human saliva. Competent S. gordonii could be transformed in vitro with plasmid DNA that had been taken into the human mouth. Transformation also occurred with a plasmid that cannot replicate in S. gordonii, but that has a region or chromosomal homology, by integration into the bacterial chromosome, although linearised plasmid DNA gave no transformants. Linear chromosomal DNA fragments did however transform S. gordonii/Tn916 efficiently in saliva when regions of homology with the recipient chromosome flanked the marker gene. These findings are discussed in relation to the potential for acquisition of DNA sequences, including genetically modified DNA, by gut and oral bacteria. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalFEMS Microbiology Letters
Volume200
Publication statusPublished - 2001

Keywords

  • transformation
  • homologous recombination
  • DNA survival
  • genetically modified DNA
  • chromosomal integration
  • Streptococcus
  • NATURAL GENETIC-TRANSFORMATION
  • STREPTOCOCCUS-SANGUIS CHALLIS
  • PSEUDOMONAS-FLUORESCENS
  • GASTROINTESTINAL-TRACT
  • FOODSTUFFS
  • PLASMIDS
  • ECOLOGY
  • MUTANS
  • MARKER
  • FOOD

Cite this

Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva. / Mercer, D K ; Scott, K P ; Melville, C M ; Glover, L A ; Flint, Harry James.

In: FEMS Microbiology Letters, Vol. 200, 2001, p. 163-167.

Research output: Contribution to journalArticle

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AU - Glover, L A

AU - Flint, Harry James

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AB - Transformation of Streptococcus gordonii DL1 by free DNA was studied in human saliva. Competent S. gordonii could be transformed in vitro with plasmid DNA that had been taken into the human mouth. Transformation also occurred with a plasmid that cannot replicate in S. gordonii, but that has a region or chromosomal homology, by integration into the bacterial chromosome, although linearised plasmid DNA gave no transformants. Linear chromosomal DNA fragments did however transform S. gordonii/Tn916 efficiently in saliva when regions of homology with the recipient chromosome flanked the marker gene. These findings are discussed in relation to the potential for acquisition of DNA sequences, including genetically modified DNA, by gut and oral bacteria. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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KW - PSEUDOMONAS-FLUORESCENS

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KW - FOOD

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JO - FEMS Microbiology Letters

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