Traversing the ovine cervix: a challenge for cryopreserved semen and creative science

J. J. Robinson, W. A. C. McKelvey, M. E. King, S. E. Mitchell, M. J. A. Mylne, T. G. McEvoy, W. S. Dingwall, Lynda Williams

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This review brings together research findings on cervical relaxation in the ewe and its pharmacological stimulation for enhancement of the penetration needed for transcervical insemination and embryo transfer. On the basis that the success of artificial insemination is the percentage of ewes lambing, a review is made of recent research aimed at understanding and minimising the sub-lethal effects of freezing and thawing on the viability of spermatozoa, their membrane integrity and their ability to migrate through cervical mucus, as these characteristics have a major influence on fertility, particularly when semen is deposited, artificially, in the os cervix. Milestones of achievement are given for transcervical intrauterine insemination, embryo recovery and transfer and the birth of lambs of pre-determined sex, firstly following intracytoplasmic sperm injection, then laparoscopic intrauterine insemination using highly diluted flow-cytometrically sorted fresh semen and subsequently by os cervix insemination using sexed semen that had been frozen and thawed. Diversity of research endeavour (applied, cellular, molecular), research discipline (anatomy, histology, immunology, endocrinology) and research focus (cell, tissue, organ, whole animal) is embraced within the review as each has significant contributions to make in advancing recent scientific findings from the laboratory into robust on-farm transcervical insemination and embryo transfer techniques.

Original languageEnglish
Pages (from-to)1791-1804
Number of pages14
JournalAnimal
Volume5
Issue number11
Early online date22 Jun 2011
DOIs
Publication statusPublished - 26 Sep 2011

Keywords

  • ovine cervix
  • semen cryopreservation
  • artificial insemination
  • embryo recovery and transfer
  • frozen-thawed semen
  • transcervical artificial-insemination
  • proteinase-activated receptors
  • seminal plasma-proteins
  • in-vitro fertilization
  • sorted ram spermatozoa
  • early luteal-phase
  • intrauterine insemination
  • embryo-transfer
  • motility characteristics

Cite this

Robinson, J. J., McKelvey, W. A. C., King, M. E., Mitchell, S. E., Mylne, M. J. A., McEvoy, T. G., Dingwall, W. S., & Williams, L. (2011). Traversing the ovine cervix: a challenge for cryopreserved semen and creative science. Animal , 5(11), 1791-1804. https://doi.org/10.1017/S1751731111000978