Trypanosoma-Brucei is Protected from the Cytostaic Effects of Nitric Oxide Under In-Vivo Conditions

N A Mabbott, I A Sutherland, Jeremy M Sternberg

Research output: Contribution to journalArticle

Abstract

In mice infected with Trypanosoma brucei, splenic and peritoneal macrophages release substantial amounts of nitric oxide (NO). The production of NO by activated macrophages has been reported to be a nonspecific immune-effector mechansism against several parasites, and in this work we investigate the role of NO in killing T. brucei. Addition of bloodstream trypanosomes to peritoneal macrophages activated in vitro resulted in an NO-dependent inhibition of parasite growth. This effect was totally abrogated when dilutions of whole blood were included in the cultures, suggesting that bloodstream parasites such as T. brucei are not susceptible to NO-mediated killing in vivo.

Original languageEnglish
Pages (from-to)687-690
Number of pages4
JournalParasitology Research
Volume80
Issue number8
Publication statusPublished - Nov 1994

Keywords

  • ACTIVATED MACROPHAGES
  • L-ARGININE
  • MURINE MACROPHAGES
  • GAMMA-INTERFERON
  • RHODESIENSE
  • INHIBITION
  • METABOLISM
  • GAMBIENSE
  • RELEASE
  • PATHWAY

Cite this

Trypanosoma-Brucei is Protected from the Cytostaic Effects of Nitric Oxide Under In-Vivo Conditions. / Mabbott, N A ; Sutherland, I A ; Sternberg, Jeremy M.

In: Parasitology Research, Vol. 80, No. 8, 11.1994, p. 687-690.

Research output: Contribution to journalArticle

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abstract = "In mice infected with Trypanosoma brucei, splenic and peritoneal macrophages release substantial amounts of nitric oxide (NO). The production of NO by activated macrophages has been reported to be a nonspecific immune-effector mechansism against several parasites, and in this work we investigate the role of NO in killing T. brucei. Addition of bloodstream trypanosomes to peritoneal macrophages activated in vitro resulted in an NO-dependent inhibition of parasite growth. This effect was totally abrogated when dilutions of whole blood were included in the cultures, suggesting that bloodstream parasites such as T. brucei are not susceptible to NO-mediated killing in vivo.",
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KW - L-ARGININE

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KW - GAMMA-INTERFERON

KW - RHODESIENSE

KW - INHIBITION

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