Tumour imaging by Positron Emission Tomography using fluorinase generated 5-[18F]fluoro-5-deoxyribose as a novel tracer

Sergio Dall'Angelo, Nouchali Bandaranayaka, Albert D. Windhorst, Danielle Vugts, Dion van der Born, Mayca Onega, Lutz F. Schweiger, Matteo Zanda, David O'Hagan

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


5-[18F]Fluoro-5-deoxyribose ([18F]FDR) 3 was prepared as a novel monosaccharide radiotracer in a two-step synthesis using the fluorinase, a C-F bond forming enzyme, and a nucleoside hydrolase. The resulting [18F]FDR 3 was then explored as a radiotracer for imaging tumours (A431 human epithelial carcinoma) by positron emission tomography in a mice model.

5-[18F]Fluoro-5-deoxyribose ([18F]FDR) 3, was prepared by incubating S-adenosyl-L-methionine (SAM) and [18F]fluoride with the fluorinase enzyme, and then incubating the product of this reaction, [18F]-5'-fluoro-5'-deoxadenosine ([18F]FDA) 2, with an adenosine hydrolase to generate [18F]FDR 3. The enzymes were freeze-dried and were used on demand by dissolution in buffer solution. The resulting [18F]FDR 3 was then administered to four mice that had tumours induced from the A431 human epithelial carcinoma cell line.

The tumour (A431 human epithelial carcinoma) bearing mice were successfully imaged with [18F]FDR 3. The radiotracer displayed good tumour imaging resolution. A direct comparison of the uptake and efflux of [18F]FDR 3 with 2-[18F]fluoro-2-deoxyglucose ([18F]FDG) was made, revealing comparative tumour uptake and imaging potential over the first 10–20 min. The study revealed however that [18F]FDR 3 does not accumulate in the tumour as efficiently as [18F]FDG over longer time periods.

[18F]FDR 3 can be rapidly synthesised in a two enzyme protocol and used to image tumours in small animal models.
Original languageEnglish
Pages (from-to)464-470
Number of pages7
JournalNuclear Medicine and Biology
Issue number4
Early online date27 Mar 2013
Publication statusPublished - May 2013


  • positron emission tomography
  • fluorine-18
  • 5-fluoro-5-deoxyribose
  • fluorinase
  • biotransformation
  • tumour mice models


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