Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

A. Kindness, C. N. Sekaran, Jorg Feldmann

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Background: Metals are not homogeneously distributed in organ tissues. Although most mapping techniques, such as histologic staining methods, have been developed for element imaging on a subcellular level, many suffer from either low precision or poor detection limits. Therefore, small variations in elemental distribution cannot be identified. We developed a method for two-dimensional mapping of trace elements to identify the influence of metabolic zonation by the liver on trace element distribution.

Methods: A prepared homogeneous Certified Reference Material (CRM; LGC 7112, pig liver) was used to characterize the laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-MS) in terms of precision. Different isotopes for copper and zinc were monitored, and the use of carbon as an internal standard was investigated to correct for differences in ablation efficiency to identify the most precise mapping technique for liver samples.

Results: For the homogeneous CRM, the reproducibility of the copper and zinc signals was similar to3-24% depending on spot size and number of pulses. When carbon was used as an internal standard, the reproducibility was improved significantly. Line scan signals over a length of 1.5 mm were more precise [relative SD (RSD), 1.6-6.1% for copper (Cu-63, Cu-65) and zinc (Zn-64, Zn-66) depending on the spot size, the scanning speed, and the element]. Thin section of sheep liver achieved precisions of 27-59% (raster scan) and 9-47% (line scan) RSD for copper, whereas the precision for zinc was significantly better: 8-18% (raster scan) and 4-21% (line scan) RSD. Long line scans and two-dimensional element maps of the thin sections revealed the zonation of copper in sheep liver containing extremely low copper concentrations.

Conclusion: Elemental mapping of trace elements generated by LA-ICP-MS can be very precise so that small changes in the elemental concentration in the tissue can be detected and nonuniform spatial distribution of the elements in tissues can be established. (C) 2003 American Association for Clinical Chemistry.

Original languageEnglish
Pages (from-to)1916-1923
Number of pages8
JournalClinical Chemistry
Volume49
Issue number11
DOIs
Publication statusPublished - Nov 2003

Fingerprint

Inductively coupled plasma mass spectrometry
Laser Therapy
Laser ablation
Liver
Zinc
Copper
Mass Spectrometry
Trace Elements
Inductively coupled plasma
Tissue
Zinc Isotopes
Mass spectrometry
Sheep
Carbon
Ablation
Isotopes
Spatial distribution
Limit of Detection
Swine
Metals

Keywords

  • X-Ray-microanalysis
  • tissue
  • sheep
  • cells
  • ion

Cite this

Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry. / Kindness, A.; Sekaran, C. N.; Feldmann, Jorg.

In: Clinical Chemistry, Vol. 49, No. 11, 11.2003, p. 1916-1923.

Research output: Contribution to journalArticle

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title = "Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry",
abstract = "Background: Metals are not homogeneously distributed in organ tissues. Although most mapping techniques, such as histologic staining methods, have been developed for element imaging on a subcellular level, many suffer from either low precision or poor detection limits. Therefore, small variations in elemental distribution cannot be identified. We developed a method for two-dimensional mapping of trace elements to identify the influence of metabolic zonation by the liver on trace element distribution.Methods: A prepared homogeneous Certified Reference Material (CRM; LGC 7112, pig liver) was used to characterize the laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-MS) in terms of precision. Different isotopes for copper and zinc were monitored, and the use of carbon as an internal standard was investigated to correct for differences in ablation efficiency to identify the most precise mapping technique for liver samples.Results: For the homogeneous CRM, the reproducibility of the copper and zinc signals was similar to3-24{\%} depending on spot size and number of pulses. When carbon was used as an internal standard, the reproducibility was improved significantly. Line scan signals over a length of 1.5 mm were more precise [relative SD (RSD), 1.6-6.1{\%} for copper (Cu-63, Cu-65) and zinc (Zn-64, Zn-66) depending on the spot size, the scanning speed, and the element]. Thin section of sheep liver achieved precisions of 27-59{\%} (raster scan) and 9-47{\%} (line scan) RSD for copper, whereas the precision for zinc was significantly better: 8-18{\%} (raster scan) and 4-21{\%} (line scan) RSD. Long line scans and two-dimensional element maps of the thin sections revealed the zonation of copper in sheep liver containing extremely low copper concentrations.Conclusion: Elemental mapping of trace elements generated by LA-ICP-MS can be very precise so that small changes in the elemental concentration in the tissue can be detected and nonuniform spatial distribution of the elements in tissues can be established. (C) 2003 American Association for Clinical Chemistry.",
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T1 - Two-Dimensional Mapping of Copper and Zinc in Liver Sections by Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

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AU - Sekaran, C. N.

AU - Feldmann, Jorg

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N2 - Background: Metals are not homogeneously distributed in organ tissues. Although most mapping techniques, such as histologic staining methods, have been developed for element imaging on a subcellular level, many suffer from either low precision or poor detection limits. Therefore, small variations in elemental distribution cannot be identified. We developed a method for two-dimensional mapping of trace elements to identify the influence of metabolic zonation by the liver on trace element distribution.Methods: A prepared homogeneous Certified Reference Material (CRM; LGC 7112, pig liver) was used to characterize the laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-MS) in terms of precision. Different isotopes for copper and zinc were monitored, and the use of carbon as an internal standard was investigated to correct for differences in ablation efficiency to identify the most precise mapping technique for liver samples.Results: For the homogeneous CRM, the reproducibility of the copper and zinc signals was similar to3-24% depending on spot size and number of pulses. When carbon was used as an internal standard, the reproducibility was improved significantly. Line scan signals over a length of 1.5 mm were more precise [relative SD (RSD), 1.6-6.1% for copper (Cu-63, Cu-65) and zinc (Zn-64, Zn-66) depending on the spot size, the scanning speed, and the element]. Thin section of sheep liver achieved precisions of 27-59% (raster scan) and 9-47% (line scan) RSD for copper, whereas the precision for zinc was significantly better: 8-18% (raster scan) and 4-21% (line scan) RSD. Long line scans and two-dimensional element maps of the thin sections revealed the zonation of copper in sheep liver containing extremely low copper concentrations.Conclusion: Elemental mapping of trace elements generated by LA-ICP-MS can be very precise so that small changes in the elemental concentration in the tissue can be detected and nonuniform spatial distribution of the elements in tissues can be established. (C) 2003 American Association for Clinical Chemistry.

AB - Background: Metals are not homogeneously distributed in organ tissues. Although most mapping techniques, such as histologic staining methods, have been developed for element imaging on a subcellular level, many suffer from either low precision or poor detection limits. Therefore, small variations in elemental distribution cannot be identified. We developed a method for two-dimensional mapping of trace elements to identify the influence of metabolic zonation by the liver on trace element distribution.Methods: A prepared homogeneous Certified Reference Material (CRM; LGC 7112, pig liver) was used to characterize the laser ablation-inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-MS) in terms of precision. Different isotopes for copper and zinc were monitored, and the use of carbon as an internal standard was investigated to correct for differences in ablation efficiency to identify the most precise mapping technique for liver samples.Results: For the homogeneous CRM, the reproducibility of the copper and zinc signals was similar to3-24% depending on spot size and number of pulses. When carbon was used as an internal standard, the reproducibility was improved significantly. Line scan signals over a length of 1.5 mm were more precise [relative SD (RSD), 1.6-6.1% for copper (Cu-63, Cu-65) and zinc (Zn-64, Zn-66) depending on the spot size, the scanning speed, and the element]. Thin section of sheep liver achieved precisions of 27-59% (raster scan) and 9-47% (line scan) RSD for copper, whereas the precision for zinc was significantly better: 8-18% (raster scan) and 4-21% (line scan) RSD. Long line scans and two-dimensional element maps of the thin sections revealed the zonation of copper in sheep liver containing extremely low copper concentrations.Conclusion: Elemental mapping of trace elements generated by LA-ICP-MS can be very precise so that small changes in the elemental concentration in the tissue can be detected and nonuniform spatial distribution of the elements in tissues can be established. (C) 2003 American Association for Clinical Chemistry.

KW - X-Ray-microanalysis

KW - tissue

KW - sheep

KW - cells

KW - ion

U2 - 10.1373/clinchem.2003.022046

DO - 10.1373/clinchem.2003.022046

M3 - Article

VL - 49

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JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

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ER -