Two interleukin-17C-like genes exist in rainbow trout Oncorhynchus mykiss that are differentially expressed and modulated

Tiehui Wang, Samuel A M Martin, Christopher J Secombes

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Interleukin (IL)-17 family members (IL-17A-F) are key players in adaptive immune responses and have a central role in coordinating innate and adaptive immunity. Here, we report on two novel IL-17 homologues in rainbow trout Oncorhynchus mykiss, trout (t) IL-17C1 and tIL-17C2, that share 73.7% amino acid identity. The two tIL-17C-like molecules have relatively higher sequence identities to IL-17Cs from fish and mammals and the fish IL-17C-like molecules phylogenetically form a specific clade that groups with the mammalian IL-17C and IL-17E clades. However, the gene organisation of the fish IL-17C-like molecules is closer to mammalian IL-17Es than to IL-17Cs, and this taken together with other factors suggest the fish IL-17C-like genes may have arisen from an ancestral gene that gave rise to mammalian IL-17C and IL-17E. The expression of tIL-17Cs was detectable in all the eight tissues examined, with the expression of tIL-17 mainly contributed by tIL-17C1 in gills and skin, and by tIL-17C2 in spleen, head kidney and brain. The expression of tIL-17Cs was modulated by inflammatory stimulants, including IL-1beta, interferon-gamma, LPS and PolyIC, in a trout macrophage cell line (RTS-11). IL-1beta was the most potent inducer of tIL-17C2 but only had a minor effect on the expression of tIL-17C1. LPS and PolyIC were also potent inducers of tIL-17C2. The expression of tIL-17Cs was also up-regulated by bacterial infection, with the extent and increase more dramatic for tIL-17C2. The broad distribution of expression and differential modulation of tIL-17Cs by inflammatory stimulants and infection suggest important roles of the two tIL-17Cs in the salmonid immune system.
Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume34
Issue number5
Early online date10 Dec 2009
DOIs
Publication statusPublished - May 2010

Keywords

  • Adaptive Immunity
  • Amino Acid Sequence
  • Animals
  • Bacterial Infections
  • Cell Line
  • Evolution, Molecular
  • Gene Duplication
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gills
  • Inflammation Mediators
  • Interleukin-17
  • Macrophages
  • Mammals
  • Molecular Sequence Data
  • Oncorhynchus mykiss
  • Organ Specificity
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Skin
  • Rainbow trout
  • IL-17C-like
  • Gene organisation
  • Bacterial infection
  • LPS
  • IL-1beta
  • Expression
  • Modulation
  • IL-17 cytokine family
  • Macrophages in-vitro
  • Functional-characterization
  • IFN-gamma
  • T-cell
  • Evolution
  • Member
  • Fish
  • Phytohemmaglutinin
  • Identification

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