Two-step delignification of miscanthus to enhance enzymatic hydrolysis: aqueous ammonia followed by sodium hydroxide and oxidants

Zhongguo Liu, Sasisanker Padmanabhan, Kun Cheng, Hongxue Xie, Amit A Gokhale, Waheed Afzal, Hui Na, Markus Pauly, Alex T Bell, John M Prausnitz

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6 Citations (Scopus)

Abstract

Pretreatment of miscanthus is essential for enzymatic production of sugars to yield bioethanol. A two-step process using 10 wt % aqueous ammonia in the first step is followed by 1 wt % sodium hydroxide (with or without oxygen or 1 wt % hydrogen peroxide) in the second step. The first step retains about 90% of the cellulose and about 67% of the hemicellulose in the solid while removing about 62% of the lignin. Both steps together achieve 83–90% delignification. Subsequent enzymatic conversion to fermentable sugars is close to 90% after 96 h. While an oxidant does not significantly increase delignification, it has a favorable effect on saccharification of the recovered solid. Infrared spectroscopy, X-ray diffraction, and two-dimensional nuclear magnetic resonance spectroscopy provide data concerning the chemical composition of the recovered solid. Inclusion of an oxidant to the pretreatment breaks β-O-4′-linked aryl ether bonds of the remaining lignin in the recovered solid.
Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalEnergy & Fuels
Volume28
Issue number1
DOIs
Publication statusPublished - 16 Jan 2014

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    Liu, Z., Padmanabhan, S., Cheng, K., Xie, H., Gokhale, A. A., Afzal, W., Na, H., Pauly, M., Bell, A. T., & Prausnitz, J. M. (2014). Two-step delignification of miscanthus to enhance enzymatic hydrolysis: aqueous ammonia followed by sodium hydroxide and oxidants. Energy & Fuels, 28(1), 542-548. https://doi.org/10.1021/ef401317z