Two-step delignification of miscanthus to enhance enzymatic hydrolysis: aqueous ammonia followed by sodium hydroxide and oxidants

Zhongguo Liu, Sasisanker Padmanabhan, Kun Cheng, Hongxue Xie, Amit A Gokhale, Waheed Afzal, Hui Na, Markus Pauly, Alex T Bell, John M Prausnitz

Research output: Contribution to journalArticle

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Abstract

Pretreatment of miscanthus is essential for enzymatic production of sugars to yield bioethanol. A two-step process using 10 wt % aqueous ammonia in the first step is followed by 1 wt % sodium hydroxide (with or without oxygen or 1 wt % hydrogen peroxide) in the second step. The first step retains about 90% of the cellulose and about 67% of the hemicellulose in the solid while removing about 62% of the lignin. Both steps together achieve 83–90% delignification. Subsequent enzymatic conversion to fermentable sugars is close to 90% after 96 h. While an oxidant does not significantly increase delignification, it has a favorable effect on saccharification of the recovered solid. Infrared spectroscopy, X-ray diffraction, and two-dimensional nuclear magnetic resonance spectroscopy provide data concerning the chemical composition of the recovered solid. Inclusion of an oxidant to the pretreatment breaks β-O-4′-linked aryl ether bonds of the remaining lignin in the recovered solid.
Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalEnergy & Fuels
Volume28
Issue number1
DOIs
Publication statusPublished - 16 Jan 2014

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Sodium Hydroxide
Delignification
Enzymatic hydrolysis
Oxidants
Ammonia
Sodium
Lignin
Sugars
Saccharification
Bioethanol
Hydrogen peroxide
Cellulose
Ether
Hydrogen Peroxide
Nuclear magnetic resonance spectroscopy
Infrared spectroscopy
Ethers
Oxygen
X ray diffraction
Chemical analysis

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Two-step delignification of miscanthus to enhance enzymatic hydrolysis : aqueous ammonia followed by sodium hydroxide and oxidants. / Liu, Zhongguo; Padmanabhan, Sasisanker; Cheng, Kun; Xie, Hongxue; Gokhale, Amit A; Afzal, Waheed; Na, Hui; Pauly, Markus; Bell, Alex T; Prausnitz, John M.

In: Energy & Fuels, Vol. 28, No. 1, 16.01.2014, p. 542-548.

Research output: Contribution to journalArticle

Liu, Z, Padmanabhan, S, Cheng, K, Xie, H, Gokhale, AA, Afzal, W, Na, H, Pauly, M, Bell, AT & Prausnitz, JM 2014, 'Two-step delignification of miscanthus to enhance enzymatic hydrolysis: aqueous ammonia followed by sodium hydroxide and oxidants' Energy & Fuels, vol. 28, no. 1, pp. 542-548. https://doi.org/10.1021/ef401317z
Liu, Zhongguo ; Padmanabhan, Sasisanker ; Cheng, Kun ; Xie, Hongxue ; Gokhale, Amit A ; Afzal, Waheed ; Na, Hui ; Pauly, Markus ; Bell, Alex T ; Prausnitz, John M. / Two-step delignification of miscanthus to enhance enzymatic hydrolysis : aqueous ammonia followed by sodium hydroxide and oxidants. In: Energy & Fuels. 2014 ; Vol. 28, No. 1. pp. 542-548.
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AU - Gokhale, Amit A

AU - Afzal, Waheed

AU - Na, Hui

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AU - Prausnitz, John M

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AB - Pretreatment of miscanthus is essential for enzymatic production of sugars to yield bioethanol. A two-step process using 10 wt % aqueous ammonia in the first step is followed by 1 wt % sodium hydroxide (with or without oxygen or 1 wt % hydrogen peroxide) in the second step. The first step retains about 90% of the cellulose and about 67% of the hemicellulose in the solid while removing about 62% of the lignin. Both steps together achieve 83–90% delignification. Subsequent enzymatic conversion to fermentable sugars is close to 90% after 96 h. While an oxidant does not significantly increase delignification, it has a favorable effect on saccharification of the recovered solid. Infrared spectroscopy, X-ray diffraction, and two-dimensional nuclear magnetic resonance spectroscopy provide data concerning the chemical composition of the recovered solid. Inclusion of an oxidant to the pretreatment breaks β-O-4′-linked aryl ether bonds of the remaining lignin in the recovered solid.

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