Type I interferon regulates the survival and functionality of B cells in rainbow trout

Ottavia Benedicenti, Tiehui Wang, Esther Morel, Irene Soleto, Christopher J. Secombes, Patricia Díaz-Rosales, Carolina Tafalla* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Interferons (IFNs) orchestrate antiviral responses in jawed vertebrates and can be classified into three types based on different aspects of their genomic organization, structure and receptors through which they signal and function. Generally, type I and type III IFNs include cytokines that directly induce an antiviral response, whereas type II IFNs are well-known for their immunomodulatory role during viral infections. In mammals, type I IFNs have been shown to also regulate many aspects of B cell development and differentiation. Yet, these functions have been only faintly investigated for teleost IFNs. Thus, in the current study, we have examined the effects of a model type I rainbow trout IFN molecule (IFNa) on blood naïve (IgM+IgD+) B cells, comparing them to those exerted by type II IFN (IFNγ). Our results demonstrate that IFNa increases the survival of naïve rainbow trout B cells, in the absence of lymphoproliferative effects, by rescuing them from spontaneous apoptosis. Additionally, IFNa increased the phagocytic capacity of blood IgM+IgD+ B cells and augmented the number of IgM-secreting cells in blood leukocyte cultures. IFNγ, on the other hand, had only minor effects up-regulating IgM secretion, whereas it increased the phagocytic capacity of IgM− cells in the cultures. Finally, given the recent identification of 9 mx genes in rainbow trout, we have also established which of these genes were transcriptionally regulated in blood naïve B cells in response to IFNa. This study points to a previously undescribed role for teleost type I IFNs in the regulation of B cell responses.

Original languageEnglish
Article number1494
Number of pages15
JournalFrontiers in Immunology
Volume11
DOIs
Publication statusPublished - 9 Jul 2020

Keywords

  • teleost fish cells
  • B cells
  • interferon (IFN)
  • IgM
  • phagocytosis
  • CYTOKINES
  • IFN-GAMMA
  • ONCORHYNCHUS-MYKISS
  • MX PROTEINS
  • IGD
  • IDENTIFICATION
  • ANTIVIRAL PROTECTION
  • GENE
  • teleost fish
  • ANTIBODY-RESPONSES
  • EXPRESSION

Fingerprint Dive into the research topics of 'Type I interferon regulates the survival and functionality of B cells in rainbow trout'. Together they form a unique fingerprint.

  • Cite this

    Benedicenti, O., Wang, T., Morel, E., Soleto, I., Secombes, C. J., Díaz-Rosales, P., & Tafalla, C. (2020). Type I interferon regulates the survival and functionality of B cells in rainbow trout. Frontiers in Immunology, 11, [1494]. https://doi.org/10.3389/fimmu.2020.01494