Ultrastructure of bronchial biopsies from patients with allergic and non-allergic asthma

S Shahana, E. Björnsson, D. Lúdvíksdóttir, C. Janson, O. Nettelbladt, P. Venge, G. M. Roomans, the BHR-group

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Abstract

Epithelial damage is commonly found in airways of asthma patients. The aim of this study was to investigate epithelial damage in allergic and non-allergic asthma at the ultrastructural level. Bronchial biopsies obtained from patients with allergic asthma (n=11), non-allergic asthma (n=7), and healthy controls (n=5) were studied by transmission electron microscopy. Epithelial damage was found to be extensive in both asthma groups. Both in basal and in columnar cells, relative desmosome length was reduced by 30-40%. In columnar cells, half-desmosomes (i.e., desmosomes of which only one side was present) were frequently noticed. Eosinophils showing piece-meal degranulation were commonly observed in allergic asthma. Degranulating mast cells were more often observed in allergic asthma. Goblet cell hyperplasia was only found in allergic asthma. Lymphocytes were increased in both groups. In both groups, the lamina densa of the basal lamina was thicker than the control by about 40-50%. In allergic asthma the lamina densa was irregular with focal thickening. While there was always a tendency for changes (epithelial damage, desmosomes, degranulating mast cells, basal lamina) to be more extensive in allergic asthma compared to non-allergic asthma, there was no significant difference between the two groups in this respect. Reduced desmosomal contact may be an important factor in the epithelial shedding observed in patients with asthma.

Original languageEnglish
Pages (from-to)429-443
Number of pages15
JournalRespiratory Medicine
Volume99
Issue number4
Early online date26 Oct 2004
DOIs
Publication statusPublished - Apr 2005
EventAmerican Thoracic Society (ATS) 99th International Conference - Seattle, United States
Duration: 16 May 200321 May 2003

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Asthma
Biopsy
Desmosomes
Basement Membrane
Mast Cells
Goblet Cells
Transmission Electron Microscopy
Eosinophils
Hyperplasia
Lymphocytes

Keywords

  • Asthma
  • Atopy
  • Desmosome
  • Epithelial damage
  • Electron microscopy

Cite this

Shahana, S., Björnsson, E., Lúdvíksdóttir, D., Janson, C., Nettelbladt, O., Venge, P., ... the BHR-group (2005). Ultrastructure of bronchial biopsies from patients with allergic and non-allergic asthma. Respiratory Medicine, 99(4), 429-443. https://doi.org/10.1016/j.rmed.2004.08.013

Ultrastructure of bronchial biopsies from patients with allergic and non-allergic asthma. / Shahana, S; Björnsson, E.; Lúdvíksdóttir, D.; Janson, C.; Nettelbladt, O.; Venge, P.; Roomans, G. M.; the BHR-group.

In: Respiratory Medicine, Vol. 99, No. 4, 04.2005, p. 429-443.

Research output: Contribution to journalArticle

Shahana, S, Björnsson, E, Lúdvíksdóttir, D, Janson, C, Nettelbladt, O, Venge, P, Roomans, GM & the BHR-group 2005, 'Ultrastructure of bronchial biopsies from patients with allergic and non-allergic asthma', Respiratory Medicine, vol. 99, no. 4, pp. 429-443. https://doi.org/10.1016/j.rmed.2004.08.013
Shahana, S ; Björnsson, E. ; Lúdvíksdóttir, D. ; Janson, C. ; Nettelbladt, O. ; Venge, P. ; Roomans, G. M. ; the BHR-group. / Ultrastructure of bronchial biopsies from patients with allergic and non-allergic asthma. In: Respiratory Medicine. 2005 ; Vol. 99, No. 4. pp. 429-443.
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abstract = "Epithelial damage is commonly found in airways of asthma patients. The aim of this study was to investigate epithelial damage in allergic and non-allergic asthma at the ultrastructural level. Bronchial biopsies obtained from patients with allergic asthma (n=11), non-allergic asthma (n=7), and healthy controls (n=5) were studied by transmission electron microscopy. Epithelial damage was found to be extensive in both asthma groups. Both in basal and in columnar cells, relative desmosome length was reduced by 30-40{\%}. In columnar cells, half-desmosomes (i.e., desmosomes of which only one side was present) were frequently noticed. Eosinophils showing piece-meal degranulation were commonly observed in allergic asthma. Degranulating mast cells were more often observed in allergic asthma. Goblet cell hyperplasia was only found in allergic asthma. Lymphocytes were increased in both groups. In both groups, the lamina densa of the basal lamina was thicker than the control by about 40-50{\%}. In allergic asthma the lamina densa was irregular with focal thickening. While there was always a tendency for changes (epithelial damage, desmosomes, degranulating mast cells, basal lamina) to be more extensive in allergic asthma compared to non-allergic asthma, there was no significant difference between the two groups in this respect. Reduced desmosomal contact may be an important factor in the epithelial shedding observed in patients with asthma.",
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AU - Nettelbladt, O.

AU - Venge, P.

AU - Roomans, G. M.

AU - the BHR-group

N1 - The expert technical assistance of Anders Ahlander, Leif Ljung and Ulrike Spetz-Nyström is gratefully acknowledged. The study was supported by grants from the Swedish Care and Allergy Foundation (Vårdal), the Swedish Association for Asthma and Allergy, the Swedish Science Research Council, the Agnes and Mac Rudberg Foundation and the Lily and Ragnar Åkerham Foundation.

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N2 - Epithelial damage is commonly found in airways of asthma patients. The aim of this study was to investigate epithelial damage in allergic and non-allergic asthma at the ultrastructural level. Bronchial biopsies obtained from patients with allergic asthma (n=11), non-allergic asthma (n=7), and healthy controls (n=5) were studied by transmission electron microscopy. Epithelial damage was found to be extensive in both asthma groups. Both in basal and in columnar cells, relative desmosome length was reduced by 30-40%. In columnar cells, half-desmosomes (i.e., desmosomes of which only one side was present) were frequently noticed. Eosinophils showing piece-meal degranulation were commonly observed in allergic asthma. Degranulating mast cells were more often observed in allergic asthma. Goblet cell hyperplasia was only found in allergic asthma. Lymphocytes were increased in both groups. In both groups, the lamina densa of the basal lamina was thicker than the control by about 40-50%. In allergic asthma the lamina densa was irregular with focal thickening. While there was always a tendency for changes (epithelial damage, desmosomes, degranulating mast cells, basal lamina) to be more extensive in allergic asthma compared to non-allergic asthma, there was no significant difference between the two groups in this respect. Reduced desmosomal contact may be an important factor in the epithelial shedding observed in patients with asthma.

AB - Epithelial damage is commonly found in airways of asthma patients. The aim of this study was to investigate epithelial damage in allergic and non-allergic asthma at the ultrastructural level. Bronchial biopsies obtained from patients with allergic asthma (n=11), non-allergic asthma (n=7), and healthy controls (n=5) were studied by transmission electron microscopy. Epithelial damage was found to be extensive in both asthma groups. Both in basal and in columnar cells, relative desmosome length was reduced by 30-40%. In columnar cells, half-desmosomes (i.e., desmosomes of which only one side was present) were frequently noticed. Eosinophils showing piece-meal degranulation were commonly observed in allergic asthma. Degranulating mast cells were more often observed in allergic asthma. Goblet cell hyperplasia was only found in allergic asthma. Lymphocytes were increased in both groups. In both groups, the lamina densa of the basal lamina was thicker than the control by about 40-50%. In allergic asthma the lamina densa was irregular with focal thickening. While there was always a tendency for changes (epithelial damage, desmosomes, degranulating mast cells, basal lamina) to be more extensive in allergic asthma compared to non-allergic asthma, there was no significant difference between the two groups in this respect. Reduced desmosomal contact may be an important factor in the epithelial shedding observed in patients with asthma.

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