Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death

S M McFarlane, H M Anderson, S J Tucker, O J Jupp, D J McEwan

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Abstract

Tumour necrosis factor-alpha (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+-chelators EGTA and BAPTA-AM as well as microsomal Ca2+-ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+](i) levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalMolecular & Cellular Biochemistry
Volume211
Issue number1-2
Publication statusPublished - Aug 2000

Keywords

  • tumour necrosis factor
  • receptors
  • subtypes
  • calcium
  • apoptosis
  • cancer
  • factor cytotoxicity
  • L929 cells
  • TNF-Alpha
  • involvement
  • CA2+
  • intranuclear
  • accumulation
  • enhancement
  • mechanisms
  • increase

Cite this

McFarlane, S. M., Anderson, H. M., Tucker, S. J., Jupp, O. J., & McEwan, D. J. (2000). Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death. Molecular & Cellular Biochemistry, 211(1-2), 19-26.