Abstract
Tumour necrosis factor-alpha (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+-chelators EGTA and BAPTA-AM as well as microsomal Ca2+-ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+](i) levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.
Original language | English |
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Pages (from-to) | 19-26 |
Number of pages | 8 |
Journal | Molecular & Cellular Biochemistry |
Volume | 211 |
Issue number | 1-2 |
Publication status | Published - Aug 2000 |
Keywords
- tumour necrosis factor
- receptors
- subtypes
- calcium
- apoptosis
- cancer
- factor cytotoxicity
- L929 cells
- TNF-Alpha
- involvement
- CA2+
- intranuclear
- accumulation
- enhancement
- mechanisms
- increase