Upland rice grown in soil-filled chambers and exposed to contrasting water-deficit regimes II. Mapping quantitative trait loci for root morphology and distribution

Adam Huw Price, K. A. Steele, B. J. Moore, R. G. W. Jones

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Root morphological characteristics are known to be important in the drought resistance of some rice (Oryza sativa L.) varieties. The identification of quantitative trait loci (QTLs) associated with root morphology and other drought resistance-related traits should help breeders produce more drought resistant varieties. Stability in the expression of root growth QTL across rooting environments is critical for their use in breeding programs. A greenhouse experiment in which a mapping population of 140 recombinant inbred lines and the parental varieties Bala and Azucena were grown in glass-sided soil chambers and evaluated for root growth and water uptake was conducted. In each of 2 years, two treatments were used; an early water-deficit (WD0) in which seeds were sown into wet soil but received no more water, and a late water-deficit (WD49) in which the plants were watered for 49 days and then received no water for a week. The major differences between treatments and years in dry matter partitioning and root growth traits are reported elsewhere. Here, the identification of QTLs for root growth traits by composite interval mapping is described. At LOD > 3.2, there were six QTLs for the weight of roots below 90 cm and maximum root length, 11 for root to shoot ratio, 12 for the number of roots past 100 cm, and 14 for root thickness. A total of 24 regions were identified as containing QTLs (these regions often contained several QTLs identified for different root traits). Some were revealed only in individual experiments and/or for individual traits, while others were common to different traits or experiments. Seven QTLs. on chromosomes 1, 2, 4, 7, 9 (two QTLs) and 11, where considered particularly noteworthy. The complex results are discussed in the context of previously reported QTLs for root growth in other populations, the interaction between QTL with the environment and the value of QTLs for breeding. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)25-43
Number of pages18
JournalField Crops Research
Volume76
DOIs
Publication statusPublished - 2002

Keywords

  • root growth
  • drought resistance
  • genotype x environment interactions
  • soil moisture
  • ORYZA-SATIVA L.
  • PENETRATION ABILITY
  • DROUGHT RESISTANCE
  • GENETIC DISSECTION
  • MOLECULAR MARKERS
  • MOISTURE REGIMES
  • QTLS
  • MAP
  • POPULATION
  • LINKAGE

Cite this

@article{5604158805524767b3b7f230315c2dad,
title = "Upland rice grown in soil-filled chambers and exposed to contrasting water-deficit regimes II. Mapping quantitative trait loci for root morphology and distribution",
abstract = "Root morphological characteristics are known to be important in the drought resistance of some rice (Oryza sativa L.) varieties. The identification of quantitative trait loci (QTLs) associated with root morphology and other drought resistance-related traits should help breeders produce more drought resistant varieties. Stability in the expression of root growth QTL across rooting environments is critical for their use in breeding programs. A greenhouse experiment in which a mapping population of 140 recombinant inbred lines and the parental varieties Bala and Azucena were grown in glass-sided soil chambers and evaluated for root growth and water uptake was conducted. In each of 2 years, two treatments were used; an early water-deficit (WD0) in which seeds were sown into wet soil but received no more water, and a late water-deficit (WD49) in which the plants were watered for 49 days and then received no water for a week. The major differences between treatments and years in dry matter partitioning and root growth traits are reported elsewhere. Here, the identification of QTLs for root growth traits by composite interval mapping is described. At LOD > 3.2, there were six QTLs for the weight of roots below 90 cm and maximum root length, 11 for root to shoot ratio, 12 for the number of roots past 100 cm, and 14 for root thickness. A total of 24 regions were identified as containing QTLs (these regions often contained several QTLs identified for different root traits). Some were revealed only in individual experiments and/or for individual traits, while others were common to different traits or experiments. Seven QTLs. on chromosomes 1, 2, 4, 7, 9 (two QTLs) and 11, where considered particularly noteworthy. The complex results are discussed in the context of previously reported QTLs for root growth in other populations, the interaction between QTL with the environment and the value of QTLs for breeding. (C) 2002 Elsevier Science B.V. All rights reserved.",
keywords = "root growth, drought resistance, genotype x environment interactions, soil moisture, ORYZA-SATIVA L., PENETRATION ABILITY, DROUGHT RESISTANCE, GENETIC DISSECTION, MOLECULAR MARKERS, MOISTURE REGIMES, QTLS, MAP, POPULATION, LINKAGE",
author = "Price, {Adam Huw} and Steele, {K. A.} and Moore, {B. J.} and Jones, {R. G. W.}",
year = "2002",
doi = "10.1016/S0378-4290(02)00010-2",
language = "English",
volume = "76",
pages = "25--43",
journal = "Field Crops Research",
issn = "0378-4290",
publisher = "Elsevier",

}

TY - JOUR

T1 - Upland rice grown in soil-filled chambers and exposed to contrasting water-deficit regimes II. Mapping quantitative trait loci for root morphology and distribution

AU - Price, Adam Huw

AU - Steele, K. A.

AU - Moore, B. J.

AU - Jones, R. G. W.

PY - 2002

Y1 - 2002

N2 - Root morphological characteristics are known to be important in the drought resistance of some rice (Oryza sativa L.) varieties. The identification of quantitative trait loci (QTLs) associated with root morphology and other drought resistance-related traits should help breeders produce more drought resistant varieties. Stability in the expression of root growth QTL across rooting environments is critical for their use in breeding programs. A greenhouse experiment in which a mapping population of 140 recombinant inbred lines and the parental varieties Bala and Azucena were grown in glass-sided soil chambers and evaluated for root growth and water uptake was conducted. In each of 2 years, two treatments were used; an early water-deficit (WD0) in which seeds were sown into wet soil but received no more water, and a late water-deficit (WD49) in which the plants were watered for 49 days and then received no water for a week. The major differences between treatments and years in dry matter partitioning and root growth traits are reported elsewhere. Here, the identification of QTLs for root growth traits by composite interval mapping is described. At LOD > 3.2, there were six QTLs for the weight of roots below 90 cm and maximum root length, 11 for root to shoot ratio, 12 for the number of roots past 100 cm, and 14 for root thickness. A total of 24 regions were identified as containing QTLs (these regions often contained several QTLs identified for different root traits). Some were revealed only in individual experiments and/or for individual traits, while others were common to different traits or experiments. Seven QTLs. on chromosomes 1, 2, 4, 7, 9 (two QTLs) and 11, where considered particularly noteworthy. The complex results are discussed in the context of previously reported QTLs for root growth in other populations, the interaction between QTL with the environment and the value of QTLs for breeding. (C) 2002 Elsevier Science B.V. All rights reserved.

AB - Root morphological characteristics are known to be important in the drought resistance of some rice (Oryza sativa L.) varieties. The identification of quantitative trait loci (QTLs) associated with root morphology and other drought resistance-related traits should help breeders produce more drought resistant varieties. Stability in the expression of root growth QTL across rooting environments is critical for their use in breeding programs. A greenhouse experiment in which a mapping population of 140 recombinant inbred lines and the parental varieties Bala and Azucena were grown in glass-sided soil chambers and evaluated for root growth and water uptake was conducted. In each of 2 years, two treatments were used; an early water-deficit (WD0) in which seeds were sown into wet soil but received no more water, and a late water-deficit (WD49) in which the plants were watered for 49 days and then received no water for a week. The major differences between treatments and years in dry matter partitioning and root growth traits are reported elsewhere. Here, the identification of QTLs for root growth traits by composite interval mapping is described. At LOD > 3.2, there were six QTLs for the weight of roots below 90 cm and maximum root length, 11 for root to shoot ratio, 12 for the number of roots past 100 cm, and 14 for root thickness. A total of 24 regions were identified as containing QTLs (these regions often contained several QTLs identified for different root traits). Some were revealed only in individual experiments and/or for individual traits, while others were common to different traits or experiments. Seven QTLs. on chromosomes 1, 2, 4, 7, 9 (two QTLs) and 11, where considered particularly noteworthy. The complex results are discussed in the context of previously reported QTLs for root growth in other populations, the interaction between QTL with the environment and the value of QTLs for breeding. (C) 2002 Elsevier Science B.V. All rights reserved.

KW - root growth

KW - drought resistance

KW - genotype x environment interactions

KW - soil moisture

KW - ORYZA-SATIVA L.

KW - PENETRATION ABILITY

KW - DROUGHT RESISTANCE

KW - GENETIC DISSECTION

KW - MOLECULAR MARKERS

KW - MOISTURE REGIMES

KW - QTLS

KW - MAP

KW - POPULATION

KW - LINKAGE

U2 - 10.1016/S0378-4290(02)00010-2

DO - 10.1016/S0378-4290(02)00010-2

M3 - Article

VL - 76

SP - 25

EP - 43

JO - Field Crops Research

JF - Field Crops Research

SN - 0378-4290

ER -