Uptake kinetics of arsenic species in rice (Oryza sativa L.) plants

M. J. Abedin, Jorg Feldmann, Andrew Alexander Meharg

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Abstract

Arsenic (As) finds its way into soils used for rice (Oryza sativa) cultivation through polluted irrigation water, and through historic contamination with As-based pesticides. As is known to be present as a number of chemical species in such soils, so we wished to investigate how these species were accumulated by rice. As species found in soil solution from a greenhouse experiment where rice was irrigated with arsenate contaminated water were arsenite, arsonate, dimethylarsinic acid, and monomethylarsonic acid. The short-term uptake kinetics for these four As species were determined in 7-d-old excised rice roots. High-affinity uptake (0-0.0532 mM) for arsenite and arsenate with eight rice varieties, covering two growing seasons, rice var, Boro (dry season) and rice var. Aman (wet season), showed that uptake of both arsenite and arsenate by Boro varieties was less than that of Aman varieties. Arsenite uptake was active, and was taken up at approximately the same rate as arsenate. Greater uptake of arsenite, compared with arsenate, was found at higher substrate concentration (low-affinity uptake system), Competitive inhibition of uptake with phosphate showed that arsenite and arsenate were taken up by different uptake systems because arsenate uptake was strongly suppressed in the presence of phosphate, whereas arsenite transport was not affected by phosphate. At a slow rate, there was a hyperbolic uptake of monomethylarsonic acid, and limited uptake of dimethylarsinic acid.

Original languageEnglish
Pages (from-to)1120-1128
Number of pages8
JournalPlant Physiology
Volume128
DOIs
Publication statusPublished - 2002

Keywords

  • HOLCUS-LANATUS L
  • PHOSPHATE-UPTAKE SYSTEM
  • LEAD-ARSENATE
  • FLOODED CONDITIONS
  • DRINKING-WATER
  • CHEMICAL FORM
  • SOIL SOLUTION
  • WEST-BENGAL
  • BANGLADESH
  • GROUNDWATER

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