Uptake of acetylated peptides from the small intestine in sheep and their nutritive value in rats

R. J. Wallace, W. K. Min, M. W. Witt, G. Grant, J. C. MacRae, C. J. Newbold, W. J. Maeng, L. M. Rode

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Abstract

Acetylation is a potential method for protecting dietary peptides from degradation by rumen micro-organisms. As a first step in determining the nutritive value of acetylated peptides, their disappearance in the small intestine of sheep and their ability to support growth in a rat bioassay were measured. N-labelled peptides were prepared from lucerne which had been grown with N-labelled (NH)SO in the absence of Rhizobium. Peptides were prepared by enzymic hydrolysis of the extracted protein. Two peptide preparations were made using different proteinase mixtures. These mixtures contained peptides with an average molecular weight of 559 and 522Da. They were treated with acetic anhydride, which resulted in 85 and 88% modification respectively, and their uptake from the small intestine was determined by injecting 1 g of untreated or acetylated peptides in a Cr-EDTA solution into the jejunum of two sheep fitted with jejunal catheters and ileal cannulas. Ileal digesta were collected and analysed for Cr and N. The uptake of dialanine (Ala) and N-acetyl-Ala were compared in a similar way. The disappearance of N from lucerne peptides was high (88 and 93% respectively) and this was not affected significantly by acetylation (86 and 87%). Corresponding values for Ala and N-acetyl-Ala were both 96%, as measured by HPLC. It was therefore concluded that acetylation did not affect the uptake of peptides from the small intestine in sheep. Two feeding trials were carried out with rats. The first trial was carried out with a protein-free diet to which was added 10% lactalbumin or 5% lactalbumin and then a mixture of methionine-free amino acids, either alone or supplemented with Met, Gly-Met or acetylated Gly-Met. The rats grew equally well on all sources of Met, but failed to grow significantly on the mixture of Met-free amino acids. In the second trial the diet contained casein as 5.9% of the basal diet. Additional casein, pancreatic casein hydrolysate (peptides) and acetylated pancreatic casein hydrolysate (acetylated peptides) were compared as sources of amino acids, at inclusion rates of 100 g/kg final diet. Feed intake was similar with casein and peptides treatments, but was depressed by 23% with acetylated peptides. Live weight gain was 15 and 75% lower with the peptides and acetylated peptides diets respectively. Addition of lysine, arginine or histidine did not restore feed intake or weight gain of rats receiving acetylated peptides, but feed intake was restored immediately when peptides replaced acetylated peptides. When intake was restricted to 9 g/d and acetylated casein hydrolysate replaced half of the protein in the diet, rats gained weight less rapidly (1.44 v. 1.09 g/d) and retained less N, such that only 0.36 of the acetylated peptide-N was calculated to remain available to the animal. This N retention compared with 0.70 for unmodified casein. Thus, the rat bioassay indicated that certain specific peptides may well be of high nutritive value following acetylation, but that there may be problems of inappetance and inefficient utilization with acetylated peptide mixtures.
Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalBritish Journal of Nutrition
Volume80
Issue number1
DOIs
Publication statusPublished - Jul 1998

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Nutritive Value
Small Intestine
Sheep
Peptides
Acetylation
Caseins
Diet
Lactalbumin
Medicago sativa
Amino Acids
Biological Assay
Weight Gain
Protein-Restricted Diet
Rhizobium

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Uptake of acetylated peptides from the small intestine in sheep and their nutritive value in rats. / Wallace, R. J.; Min, W. K.; Witt, M. W.; Grant, G.; MacRae, J. C.; Newbold, C. J.; Maeng, W. J.; Rode, L. M.

In: British Journal of Nutrition, Vol. 80, No. 1, 07.1998, p. 101-108.

Research output: Contribution to journalArticle

Wallace, RJ, Min, WK, Witt, MW, Grant, G, MacRae, JC, Newbold, CJ, Maeng, WJ & Rode, LM 1998, 'Uptake of acetylated peptides from the small intestine in sheep and their nutritive value in rats', British Journal of Nutrition, vol. 80, no. 1, pp. 101-108. https://doi.org/10.1017/S0007114598001822
Wallace, R. J. ; Min, W. K. ; Witt, M. W. ; Grant, G. ; MacRae, J. C. ; Newbold, C. J. ; Maeng, W. J. ; Rode, L. M. / Uptake of acetylated peptides from the small intestine in sheep and their nutritive value in rats. In: British Journal of Nutrition. 1998 ; Vol. 80, No. 1. pp. 101-108.
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abstract = "Acetylation is a potential method for protecting dietary peptides from degradation by rumen micro-organisms. As a first step in determining the nutritive value of acetylated peptides, their disappearance in the small intestine of sheep and their ability to support growth in a rat bioassay were measured. N-labelled peptides were prepared from lucerne which had been grown with N-labelled (NH)SO in the absence of Rhizobium. Peptides were prepared by enzymic hydrolysis of the extracted protein. Two peptide preparations were made using different proteinase mixtures. These mixtures contained peptides with an average molecular weight of 559 and 522Da. They were treated with acetic anhydride, which resulted in 85 and 88{\%} modification respectively, and their uptake from the small intestine was determined by injecting 1 g of untreated or acetylated peptides in a Cr-EDTA solution into the jejunum of two sheep fitted with jejunal catheters and ileal cannulas. Ileal digesta were collected and analysed for Cr and N. The uptake of dialanine (Ala) and N-acetyl-Ala were compared in a similar way. The disappearance of N from lucerne peptides was high (88 and 93{\%} respectively) and this was not affected significantly by acetylation (86 and 87{\%}). Corresponding values for Ala and N-acetyl-Ala were both 96{\%}, as measured by HPLC. It was therefore concluded that acetylation did not affect the uptake of peptides from the small intestine in sheep. Two feeding trials were carried out with rats. The first trial was carried out with a protein-free diet to which was added 10{\%} lactalbumin or 5{\%} lactalbumin and then a mixture of methionine-free amino acids, either alone or supplemented with Met, Gly-Met or acetylated Gly-Met. The rats grew equally well on all sources of Met, but failed to grow significantly on the mixture of Met-free amino acids. In the second trial the diet contained casein as 5.9{\%} of the basal diet. Additional casein, pancreatic casein hydrolysate (peptides) and acetylated pancreatic casein hydrolysate (acetylated peptides) were compared as sources of amino acids, at inclusion rates of 100 g/kg final diet. Feed intake was similar with casein and peptides treatments, but was depressed by 23{\%} with acetylated peptides. Live weight gain was 15 and 75{\%} lower with the peptides and acetylated peptides diets respectively. Addition of lysine, arginine or histidine did not restore feed intake or weight gain of rats receiving acetylated peptides, but feed intake was restored immediately when peptides replaced acetylated peptides. When intake was restricted to 9 g/d and acetylated casein hydrolysate replaced half of the protein in the diet, rats gained weight less rapidly (1.44 v. 1.09 g/d) and retained less N, such that only 0.36 of the acetylated peptide-N was calculated to remain available to the animal. This N retention compared with 0.70 for unmodified casein. Thus, the rat bioassay indicated that certain specific peptides may well be of high nutritive value following acetylation, but that there may be problems of inappetance and inefficient utilization with acetylated peptide mixtures.",
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T1 - Uptake of acetylated peptides from the small intestine in sheep and their nutritive value in rats

AU - Wallace, R. J.

AU - Min, W. K.

AU - Witt, M. W.

AU - Grant, G.

AU - MacRae, J. C.

AU - Newbold, C. J.

AU - Maeng, W. J.

AU - Rode, L. M.

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N2 - Acetylation is a potential method for protecting dietary peptides from degradation by rumen micro-organisms. As a first step in determining the nutritive value of acetylated peptides, their disappearance in the small intestine of sheep and their ability to support growth in a rat bioassay were measured. N-labelled peptides were prepared from lucerne which had been grown with N-labelled (NH)SO in the absence of Rhizobium. Peptides were prepared by enzymic hydrolysis of the extracted protein. Two peptide preparations were made using different proteinase mixtures. These mixtures contained peptides with an average molecular weight of 559 and 522Da. They were treated with acetic anhydride, which resulted in 85 and 88% modification respectively, and their uptake from the small intestine was determined by injecting 1 g of untreated or acetylated peptides in a Cr-EDTA solution into the jejunum of two sheep fitted with jejunal catheters and ileal cannulas. Ileal digesta were collected and analysed for Cr and N. The uptake of dialanine (Ala) and N-acetyl-Ala were compared in a similar way. The disappearance of N from lucerne peptides was high (88 and 93% respectively) and this was not affected significantly by acetylation (86 and 87%). Corresponding values for Ala and N-acetyl-Ala were both 96%, as measured by HPLC. It was therefore concluded that acetylation did not affect the uptake of peptides from the small intestine in sheep. Two feeding trials were carried out with rats. The first trial was carried out with a protein-free diet to which was added 10% lactalbumin or 5% lactalbumin and then a mixture of methionine-free amino acids, either alone or supplemented with Met, Gly-Met or acetylated Gly-Met. The rats grew equally well on all sources of Met, but failed to grow significantly on the mixture of Met-free amino acids. In the second trial the diet contained casein as 5.9% of the basal diet. Additional casein, pancreatic casein hydrolysate (peptides) and acetylated pancreatic casein hydrolysate (acetylated peptides) were compared as sources of amino acids, at inclusion rates of 100 g/kg final diet. Feed intake was similar with casein and peptides treatments, but was depressed by 23% with acetylated peptides. Live weight gain was 15 and 75% lower with the peptides and acetylated peptides diets respectively. Addition of lysine, arginine or histidine did not restore feed intake or weight gain of rats receiving acetylated peptides, but feed intake was restored immediately when peptides replaced acetylated peptides. When intake was restricted to 9 g/d and acetylated casein hydrolysate replaced half of the protein in the diet, rats gained weight less rapidly (1.44 v. 1.09 g/d) and retained less N, such that only 0.36 of the acetylated peptide-N was calculated to remain available to the animal. This N retention compared with 0.70 for unmodified casein. Thus, the rat bioassay indicated that certain specific peptides may well be of high nutritive value following acetylation, but that there may be problems of inappetance and inefficient utilization with acetylated peptide mixtures.

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