Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea

Timothy Andrew Davies Smith; Jenny Titley

doi:10.1016/S0304-3835(99)00075-0

Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea

Timothy Andrew Davies Smith, Jenny Titley

Applied Medicine

Institute of Cancer Research

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-d-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.

Original language	English
Pages (from-to)	85-91
Number of pages	7
Journal	Cancer Letters
Volume	141
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(99)00075-0
Publication status	Published - 1 Jan 1999

Keywords

tumour
proliferation
hydroxyurea
deoxyglucose

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abstract = "SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-d-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.",

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T1 - Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea

AU - Smith, Timothy Andrew Davies

AU - Titley, Jenny

PY - 1999/1/1

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N2 - SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-d-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.

AB - SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-d-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.

KW - tumour

KW - proliferation

KW - hydroxyurea

KW - deoxyglucose

U2 - 10.1016/S0304-3835(99)00075-0

DO - 10.1016/S0304-3835(99)00075-0

M3 - Article

SN - 0304-3835

VL - 141

SP - 85

EP - 91

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea

Abstract

Keywords

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