SW620 cells were grown in tissue culture flasks to various cell densities producing populations of cells with a range of proliferative indices. The uptake of the two glucose analogues, deoxy-d-glucose (DG) and 3-O-methylglucose (OMG) was determined and found to be associated with S-phase fraction. The strong correlation between DG and OMG uptakes suggested that proliferation-related changes in transmembrane transport accounted for the association with S-phase fraction. Treatment of SW620 cells with the cell cycle inhibitor hydroxyurea was found to increase the uptake of DG and OMG in a time-dependent manner.
Smith, T. A. D., & Titley, J. (1999). Uptake of glucose analogues by colonic tumour cells during growth and after treatment with hydroxyurea. Cancer Letters, 141(1-2), 85-91. https://doi.org/10.1016/S0304-3835(99)00075-0