Uptake of long chain fatty acids by human placental choriocarcinoma (BeWo) cells

Fiona Margaret Campbell, A M Clohessy, Margaret Jane Gordon, Kenneth R Page, Asim K Dutta-Roy

Research output: Contribution to journalArticle

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Abstract

In order to understand the mechanisms by which fatty acids are taken up by the placenta, the uptake of oleic, linoleic, arachidonic, and docosahexaenoic acids by cultured human placental choriocarcinoma (BeWo) cells was examined. Fatty acid uptake by BeWo cells was temperature-dependent and exhibited saturable kinetics. Oleic acid was taken up least and docosahexaenoic acid most by these cells. Moreover, competitive studies of fatty acid uptake by BeWo cells also indicated preferential uptake compared with oleic acid in the order of docosahexaenoic acid, arachidonic acid, and linoleic acid. Western blot analysis demonstrated that BeWo cells express a protein immunoreactive with antibodies to the human placental plasma membrane fatty acid-binding protein (p-FABP(pm)). Furthermore, pre-treatment of BeWo cells with these antibodies inhibited most of the uptake of docosahexaenoic (64%) and arachidonic acids (68%) whereas oleic acid uptake was inhibited only 32% compared with the controls treated with preimmune serum. These results clearly demonstrate that the p-FABP(pm) may be involved in the preferential uptake of essential fatty acids (EFA) and their long chain polyunsaturated fatty acids (LCPUFA) by these cells. Studies on the distribution of radiolabeled fatty acids in the cellular lipids of BeWo cells showed that docosahexaenoic acid was incorporated mainly in the triacylglycerol fraction, followed by the phospholipid fraction, whereas for arachidonic acid the reverse was true. The preferential incorporation of docosahexaenoic acid into triacylglycerol suggests that triacylglycerol may play an important role in the placental transport of docosahexaenoic acid to the fetal circulation. Together these results demonstrate the preferential uptake of EFA/LCPUFA by BeWo cells that is most probably mediated via the p-FABP(pm). We thus propose that the p-FABP(pm) may be involved in the sequestration of maternal plasma LCPUFA by the placenta.

Original languageEnglish
Pages (from-to)2558-2568
Number of pages11
JournalJournal of Lipid Research
Volume38
Issue number12
Publication statusPublished - Dec 1997

Keywords

  • fatty acid uptake
  • p-FABP(pm)
  • placenta
  • BeWo cells
  • long chain polyunsaturated fatty acids
  • HUMAN HEPATOMA-CELL
  • DOCOSAHEXAENOIC ACID
  • LINOLEIC-ACID
  • RAT-BRAIN
  • METABOLISM
  • TRANSPORT
  • DIFFERENTIATION
  • LIVER
  • IDENTIFICATION
  • REQUIREMENTS
  • fatty acid uptake
  • placenta
  • long chain polyunsaturated fatty acids
  • human hepatoma-cell
  • docosahexaenoic acid
  • linoleic acid
  • rat-brain
  • metabolism
  • transport
  • differentiation
  • liver
  • identification
  • requirements

Cite this

Uptake of long chain fatty acids by human placental choriocarcinoma (BeWo) cells. / Campbell, Fiona Margaret; Clohessy, A M ; Gordon, Margaret Jane; Page, Kenneth R; Dutta-Roy, Asim K.

In: Journal of Lipid Research, Vol. 38, No. 12, 12.1997, p. 2558-2568.

Research output: Contribution to journalArticle

Campbell, FM, Clohessy, AM, Gordon, MJ, Page, KR & Dutta-Roy, AK 1997, 'Uptake of long chain fatty acids by human placental choriocarcinoma (BeWo) cells', Journal of Lipid Research, vol. 38, no. 12, pp. 2558-2568.
Campbell, Fiona Margaret ; Clohessy, A M ; Gordon, Margaret Jane ; Page, Kenneth R ; Dutta-Roy, Asim K. / Uptake of long chain fatty acids by human placental choriocarcinoma (BeWo) cells. In: Journal of Lipid Research. 1997 ; Vol. 38, No. 12. pp. 2558-2568.
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T1 - Uptake of long chain fatty acids by human placental choriocarcinoma (BeWo) cells

AU - Campbell, Fiona Margaret

AU - Clohessy, A M

AU - Gordon, Margaret Jane

AU - Page, Kenneth R

AU - Dutta-Roy, Asim K

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N2 - In order to understand the mechanisms by which fatty acids are taken up by the placenta, the uptake of oleic, linoleic, arachidonic, and docosahexaenoic acids by cultured human placental choriocarcinoma (BeWo) cells was examined. Fatty acid uptake by BeWo cells was temperature-dependent and exhibited saturable kinetics. Oleic acid was taken up least and docosahexaenoic acid most by these cells. Moreover, competitive studies of fatty acid uptake by BeWo cells also indicated preferential uptake compared with oleic acid in the order of docosahexaenoic acid, arachidonic acid, and linoleic acid. Western blot analysis demonstrated that BeWo cells express a protein immunoreactive with antibodies to the human placental plasma membrane fatty acid-binding protein (p-FABP(pm)). Furthermore, pre-treatment of BeWo cells with these antibodies inhibited most of the uptake of docosahexaenoic (64%) and arachidonic acids (68%) whereas oleic acid uptake was inhibited only 32% compared with the controls treated with preimmune serum. These results clearly demonstrate that the p-FABP(pm) may be involved in the preferential uptake of essential fatty acids (EFA) and their long chain polyunsaturated fatty acids (LCPUFA) by these cells. Studies on the distribution of radiolabeled fatty acids in the cellular lipids of BeWo cells showed that docosahexaenoic acid was incorporated mainly in the triacylglycerol fraction, followed by the phospholipid fraction, whereas for arachidonic acid the reverse was true. The preferential incorporation of docosahexaenoic acid into triacylglycerol suggests that triacylglycerol may play an important role in the placental transport of docosahexaenoic acid to the fetal circulation. Together these results demonstrate the preferential uptake of EFA/LCPUFA by BeWo cells that is most probably mediated via the p-FABP(pm). We thus propose that the p-FABP(pm) may be involved in the sequestration of maternal plasma LCPUFA by the placenta.

AB - In order to understand the mechanisms by which fatty acids are taken up by the placenta, the uptake of oleic, linoleic, arachidonic, and docosahexaenoic acids by cultured human placental choriocarcinoma (BeWo) cells was examined. Fatty acid uptake by BeWo cells was temperature-dependent and exhibited saturable kinetics. Oleic acid was taken up least and docosahexaenoic acid most by these cells. Moreover, competitive studies of fatty acid uptake by BeWo cells also indicated preferential uptake compared with oleic acid in the order of docosahexaenoic acid, arachidonic acid, and linoleic acid. Western blot analysis demonstrated that BeWo cells express a protein immunoreactive with antibodies to the human placental plasma membrane fatty acid-binding protein (p-FABP(pm)). Furthermore, pre-treatment of BeWo cells with these antibodies inhibited most of the uptake of docosahexaenoic (64%) and arachidonic acids (68%) whereas oleic acid uptake was inhibited only 32% compared with the controls treated with preimmune serum. These results clearly demonstrate that the p-FABP(pm) may be involved in the preferential uptake of essential fatty acids (EFA) and their long chain polyunsaturated fatty acids (LCPUFA) by these cells. Studies on the distribution of radiolabeled fatty acids in the cellular lipids of BeWo cells showed that docosahexaenoic acid was incorporated mainly in the triacylglycerol fraction, followed by the phospholipid fraction, whereas for arachidonic acid the reverse was true. The preferential incorporation of docosahexaenoic acid into triacylglycerol suggests that triacylglycerol may play an important role in the placental transport of docosahexaenoic acid to the fetal circulation. Together these results demonstrate the preferential uptake of EFA/LCPUFA by BeWo cells that is most probably mediated via the p-FABP(pm). We thus propose that the p-FABP(pm) may be involved in the sequestration of maternal plasma LCPUFA by the placenta.

KW - fatty acid uptake

KW - p-FABP(pm)

KW - placenta

KW - BeWo cells

KW - long chain polyunsaturated fatty acids

KW - HUMAN HEPATOMA-CELL

KW - DOCOSAHEXAENOIC ACID

KW - LINOLEIC-ACID

KW - RAT-BRAIN

KW - METABOLISM

KW - TRANSPORT

KW - DIFFERENTIATION

KW - LIVER

KW - IDENTIFICATION

KW - REQUIREMENTS

KW - fatty acid uptake

KW - placenta

KW - long chain polyunsaturated fatty acids

KW - human hepatoma-cell

KW - docosahexaenoic acid

KW - linoleic acid

KW - rat-brain

KW - metabolism

KW - transport

KW - differentiation

KW - liver

KW - identification

KW - requirements

M3 - Article

VL - 38

SP - 2558

EP - 2568

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 12

ER -