Ura-status-dependent adhesion of Candida albicans mutants

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Abstract

Gene disruptions in the diploid opportunistic human fungal pathogen Candida albicans are usually created using multiple rounds of targeted integration called the 'ura-blaster' method. Resulting heterozygous and homozygous null mutants can be auxotrophic (Ura-) or prototrophic (Ural) for uracil biosynthesis. Here we demonstrate that the Ura-status of otherwise isogenic mutants affected the adhesion of C albicans. Moreover the effect of Ura-status on adhesion was also dependent on the null mutant background, the nature of the underlying surface and the carbon source for growth. Therefore the Ura-status is not neutral in determining adhesive properties of C albicans mutants that are generated via the ura-blaster protocol. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)323-328
Number of pages5
JournalFEMS Microbiology Letters
Volume204
Issue number2
DOIs
Publication statusPublished - Nov 2001

Keywords

  • adhesion
  • gene disruption
  • medical mycology
  • mutagenesis
  • Candida albicans
  • DIFFERENT CARBON-SOURCES
  • BUCCAL EPITHELIAL-CELLS
  • AUXOTROPHIC MUTANTS
  • GENE DISRUPTION
  • EXPERIMENTAL INFECTIONS
  • PROTEINASE GENES
  • ADHERENCE
  • VIRULENCE
  • STRAINS
  • GROWTH

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