Use of ovary culture techniques in reproductive toxicology

Agnes Stefansdottir, Paul A Fowler, Nicola Powles-Glover, Richard A Anderson, Norah Spears

Research output: Contribution to journalLiterature review

20 Citations (Scopus)
4 Downloads (Pure)

Abstract

There is increasing evidence to indicate that a substantial number of both man-made and naturally occurring chemicals are disruptive to human and wildlife reproductive health. Currently, reproductive toxicology testing is primarily carried out in vivo, however, in the past 50 years, various culture methods have been developed with the aim of growing ovarian follicles in vitro. These culture systems have become a widely used tool in reproductive biology and toxicology. In this review we describe how reproductive toxicology of the ovary is greatly enhanced by in vitro studies. Experiments using in vitro ovarian cultures to understand or detect damage to the ovary itself and to its specialised structures of the follicles and oocytes, allows for faster screening of potential developmental and/or reproductive toxicants.

Original languageEnglish
Pages (from-to)117-135
Number of pages19
JournalReproductive Toxicology
Volume49
Early online date19 Aug 2014
DOIs
Publication statusPublished - Nov 2014

Fingerprint

Culture Techniques
Toxicology
Ovary
Screening
Health
Testing
Ovarian Follicle
Experiments
Reproductive Health
Oocytes
In Vitro Techniques

Keywords

  • ovary
  • follicle
  • tissue culture
  • in vitro
  • toxicology
  • pharmaceutical
  • EDC

Cite this

Use of ovary culture techniques in reproductive toxicology. / Stefansdottir, Agnes; Fowler, Paul A; Powles-Glover, Nicola; Anderson, Richard A; Spears, Norah.

In: Reproductive Toxicology, Vol. 49, 11.2014, p. 117-135.

Research output: Contribution to journalLiterature review

Stefansdottir, A, Fowler, PA, Powles-Glover, N, Anderson, RA & Spears, N 2014, 'Use of ovary culture techniques in reproductive toxicology', Reproductive Toxicology, vol. 49, pp. 117-135. https://doi.org/10.1016/j.reprotox.2014.08.001
Stefansdottir, Agnes ; Fowler, Paul A ; Powles-Glover, Nicola ; Anderson, Richard A ; Spears, Norah. / Use of ovary culture techniques in reproductive toxicology. In: Reproductive Toxicology. 2014 ; Vol. 49. pp. 117-135.
@article{3cf35631697d4ca59732b7bfce80bf0c,
title = "Use of ovary culture techniques in reproductive toxicology",
abstract = "There is increasing evidence to indicate that a substantial number of both man-made and naturally occurring chemicals are disruptive to human and wildlife reproductive health. Currently, reproductive toxicology testing is primarily carried out in vivo, however, in the past 50 years, various culture methods have been developed with the aim of growing ovarian follicles in vitro. These culture systems have become a widely used tool in reproductive biology and toxicology. In this review we describe how reproductive toxicology of the ovary is greatly enhanced by in vitro studies. Experiments using in vitro ovarian cultures to understand or detect damage to the ovary itself and to its specialised structures of the follicles and oocytes, allows for faster screening of potential developmental and/or reproductive toxicants.",
keywords = "ovary, follicle, tissue culture, in vitro, toxicology, pharmaceutical, EDC",
author = "Agnes Stefansdottir and Fowler, {Paul A} and Nicola Powles-Glover and Anderson, {Richard A} and Norah Spears",
note = "Copyright {\circledC} 2014 The Authors. Published by Elsevier Inc. All rights reserved. Acknowledgements The author's studies in this field are supported by MRC grants G1002118 (NS and RAA) and G110357 (RAA), MR/L010011/1 (PAF), the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 212885 (PAF) and the Wellcome Trust (080388 to PAF). AS was funded by a BBSRC CASE Studentship co-funded by AstraZeneca.",
year = "2014",
month = "11",
doi = "10.1016/j.reprotox.2014.08.001",
language = "English",
volume = "49",
pages = "117--135",
journal = "Reproductive Toxicology",
issn = "0890-6238",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Use of ovary culture techniques in reproductive toxicology

AU - Stefansdottir, Agnes

AU - Fowler, Paul A

AU - Powles-Glover, Nicola

AU - Anderson, Richard A

AU - Spears, Norah

N1 - Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved. Acknowledgements The author's studies in this field are supported by MRC grants G1002118 (NS and RAA) and G110357 (RAA), MR/L010011/1 (PAF), the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 212885 (PAF) and the Wellcome Trust (080388 to PAF). AS was funded by a BBSRC CASE Studentship co-funded by AstraZeneca.

PY - 2014/11

Y1 - 2014/11

N2 - There is increasing evidence to indicate that a substantial number of both man-made and naturally occurring chemicals are disruptive to human and wildlife reproductive health. Currently, reproductive toxicology testing is primarily carried out in vivo, however, in the past 50 years, various culture methods have been developed with the aim of growing ovarian follicles in vitro. These culture systems have become a widely used tool in reproductive biology and toxicology. In this review we describe how reproductive toxicology of the ovary is greatly enhanced by in vitro studies. Experiments using in vitro ovarian cultures to understand or detect damage to the ovary itself and to its specialised structures of the follicles and oocytes, allows for faster screening of potential developmental and/or reproductive toxicants.

AB - There is increasing evidence to indicate that a substantial number of both man-made and naturally occurring chemicals are disruptive to human and wildlife reproductive health. Currently, reproductive toxicology testing is primarily carried out in vivo, however, in the past 50 years, various culture methods have been developed with the aim of growing ovarian follicles in vitro. These culture systems have become a widely used tool in reproductive biology and toxicology. In this review we describe how reproductive toxicology of the ovary is greatly enhanced by in vitro studies. Experiments using in vitro ovarian cultures to understand or detect damage to the ovary itself and to its specialised structures of the follicles and oocytes, allows for faster screening of potential developmental and/or reproductive toxicants.

KW - ovary

KW - follicle

KW - tissue culture

KW - in vitro

KW - toxicology

KW - pharmaceutical

KW - EDC

U2 - 10.1016/j.reprotox.2014.08.001

DO - 10.1016/j.reprotox.2014.08.001

M3 - Literature review

VL - 49

SP - 117

EP - 135

JO - Reproductive Toxicology

JF - Reproductive Toxicology

SN - 0890-6238

ER -